BBa_M10024 1 BBa_M10024 {a~Int-native>} Intimin (native) 2009-05-08T11:00:00Z 2015-05-08T01:13:51Z Genomic sequence of E. coli strain 0157:H7 TBA false false _271_ 0 4911 271 Not in stock true Construction of {a~Inv-native>} basic part PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A) PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B) ---------------------------- PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI) Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10024 {a~Inv-native>} ---------------------------- Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAACTCATTTGCAAATGG Bhs002R Removing the EcoRI site from {a~Inv-native>} CCATTTGCAAATGAGTTCTGATTAAC Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC false Hank Shih BBa_M10024_sequence 1 taacatgattactcatggttgttatacccggacccggcacaagcataagctaaaaaaaacattgattatgcttagtgctggtttaggattgtttttttatgttaatcagaactcatttgcaaatggtgaaaattattttaaattgggttcggattcaaaactgttaactcatgatagctatcagaatcgccttttttatacgttgaaaactggtgaaactgttgccgatctttctaaatcgcaagatattaatttatcgacgatttggtcgttgaataagcatttatacagttctgaaagcgaaatgatgaaggccgcgcctggtcagcagatcattttgccactcaaaaaacttccctttgaatacagtgcactaccacttttaggttcggcacctcttgttgctgcaggtggtgttgctggtcacacgaataaactgactaaaatgtccccggacgtgaccaaaagcaacatgaccgatgacaaggcattaaattatgcggcacaacaggcggcgagtctcggtagccagcttcagtcgcgatctctgaacggcgattacgcgaaagataccgctcttggtatcgctggtaaccaggcttcgtcacagttgcaggcctggttacaacattatggaacggcagaggttaatctgcagagtggtaataactttgacggtagttcactggacttcttattaccgttctatgattccgaaaaaatgctggcatttggtcaggtcggagcgcgttacattgactcccgctttacggcaaatttaggtgcgggtcagcgttttttccttcctgcaaacatgttgggctataacgtcttcattgatcaggatttttctggtgataatacccgtttaggtattggtggcgaatactggcgagactatttcaaaagtagcgttaacggctatttccgcatgagcggctggcatgagtcatacaataagaaagactatgatgagcgcccagcaaatggcttcgatatccgttttaatggctatctaccgtcatatccggcattaggcgccaagctgatatatgagcagtattatggtgataatgttgctttgtttaattctgataagctgcagtcgaatcctggtgcggcgaccgttggtgtaaactatactccgattcctctggtgacgatggggatcgattaccgtcatggtacgggtaatgaaaatgatctcctttactcaatgcagttccgttatcagtttgataaatcgtggtctcagcaaattgaaccacagtatgttaacgagttaagaacattatcaggcagccgttacgatctggttcagcgtaataacaatattattctggagtacaagaagcaggatattctttctctgaatattccgcatgatattaatggtactgaacacagtacgcagaagattcagttgatcgttaagagcaaatacggtctggatcgtatcgtctgggatgatagtgcattacgcagtcagggcggtcagattcagcatagcggaagccaaagcgcacaagactaccaggctattttgcctgcttatgtgcaaggtggcagcaatatttataaagtgacggctcgcgcctatgaccgtaatggcaatagctctaacaatgtacagcttactattaccgttctgtcgaatggtcaagttgtcgaccaggttggggtaacggactttacggcggataagacttcggctaaagcggataacgccgataccattacttataccgcgacggtgaaaaagaatggggtagctcaggctaatgtccctgtttcatttaatattgtttcaggaactgcaactcttggggcaaatagtgccaaaacggatgctaacggtaaggcaaccgtaacgttgaagtcgagtacgccaggacaggtcgtcgtgtctgctaaaaccgcggagatgacttcagcacttaatgccagtgcggttatattttttgatcaaaccaaggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z