BBa_M10052 1 BBa_M10052 {˂ehaB!} 2009-05-05T11:00:00Z 2015-05-08T01:13:51Z Genome of Enterohemorrhagic Escherichia Coli O157:H7 EDL933 The ehaB part that codes for an autotransporter protein that is on the cell surface. The part can self transport across the bacterial membrane and go to the cell surface. The ehaB part, like other autotransporter proteins, have an N-terminal signal sequence, a passenger domain, and a translocation domain. The ehaB part has also been shown to form biofilms on polystyrene surfaces. The pcr product of the ehaB gene can be digested with XhoI/EcoRI and HindIII and ligated to XhoI/EcoRI-HindIII plasmid pBAD/MycHisA. The expression of the ehaB gene can be placed under control of araBAD promoter. Kanamycin cassette from pUC4K can be inserted into the HindIII site to enable transformation into OS56. false false _271_ 0 4742 271 Not in stock true N/A false Susan Chen BBa_M10052_sequence 1 gatctggcttaatcgcaatgaactctgatggtcacctgactattaacggtaacggcgataacgacaacactgcttctatcgaagctggtcagaacgaagttgataacaacggtgaccatgttgcagccgcgaccggtaactacaaagttcgtatcgacaacgctactggtgctggttctatcgctgactacaacggcaacgagctgatctacgtcaacgacaaaaacagcaacgcgaccttctctgctgctaacaaagctgacctgggtgcatacacctatcaggctgaacagcgcggtaacaccgttgttctgcaacagatggagttgaccgactacgctaacatggcgctgagcatcccatctgcgaacaccaatatctggaacctggaacaagacaccgttggtactcgtttgaccaactctcgtcatggcctggctgataacggcggcgcatgggtaagctacttcggtggtaacttcaacggcgacaacggcaccatcaactatgatcaggatgttaacggcatcatggtcggtgttgataccaaaattgacggtaacaacgctaagtggatcgtcggtgcggctgcaggcttcgctaaaggtgacatgaatgaccgttctggtcaggtggatcaagacagccagactgcctacatctactcttctgctcacttcgcgaacaacgtctttgttgatggtagcttgagctactctcacttcaacaacgacctgtctgcaaccatgagcaacggtacttacgttgacggtagcaccaactccgacgcttggggcttcggtttgaaagccggttacgacttcaaactgggtgatgctggttacgtgactccttacgcagcatttctggtctgttccagtctggtgatgactaccagctgagcaacgacatgaaagttgacggtcagtcttacgacagcatgcgttatgaactgggtgtagatgcaggttataccttcacctacagcgaagatcaggctctgactccgtacttcaaactggcttacgtctacgacgactctaacaacgataacgatgtgaacggtgattccatcgataacggtactgaagggtctgcggtacgtgttggtctgggtactcagttcagcttcaccaagaacttcagcgcctataccgatgctaactacctcggtggtggtgacgtagatcaagactggtccgcgaacgtgggtgttaaatatacctggtaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z