LOCUS BBa_M1006 3699 bp DNA linear 22-Dec-2024 DEFINITION Promoter, RBS, HlyB, RBS, HlyD, Term ACCESSION BBa_M1006 VERSION BBa_M1006.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..57 /label=BBa_B0035 misc_feature 1..57 /label=BBa_M1005 misc_feature 64..66 /label=Start Codon misc_feature 2185..2187 /label=Stop Codon CDS 64..2184 /label=HlyB CDS 64..2187 /label=BBa_M1003 RBS 2196..2209 /label=BBa_B0035 misc_feature 2216..2218 /label=Start Codon CDS 2216..3649 /label=HlyD misc_feature 3650..3652 /label=Stop Codon CDS 2216..3652 /label=BBa_M1004 misc_feature 3661..3699 /label=B1006 stem_loop 3670..3691 /label=modified thr terminator polyA_site 3692..3699 /label=PolyA polyA_site 3661..3669 /label=PolyA terminator 3661..3699 /label=BBa_B1006 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaatac 61 tagatggatt cctgtcacaa gattgattac ggcctgtatg cactggaaat cctggcacaa 121 tatcataacg tctccgtcaa cccggaggaa attaaacatc gcttcgatac tgacggcact 181 ggcctgggtc tgaccagctg gctgctggca gcgaagtccc tggagctgaa ggtaaagcaa 241 gtgaaaaaga cgattgatcg tctgaacttc atcagcctgc cggcactggt ttggcgtgaa 301 gatggccgtc acttcatcct gacgaaagtg tccaaagaag cgaaccgcta cctgatcttt 361 gatctggagc agcgcaaccc gcgcgtactg gaacagagcg agttcgaggc tctgtatcag 421 ggccacatca ttctgattgc atctcgttct tctgttgcag gtaaactggc gaaattcgac 481 ttcacctggt tcattccggc tattatcaag taccgtcgta ttttcatcga aaccctggtt 541 gtatctgttt tcctgcaact gttcgctctg attactccgc tgttctttca ggttgtcatg 601 gacaaagttc tggttcaccg tggcttttcc accctgaacg tcattactgt tgcgctgagc 661 gtggttgttg tgttcgaaat cattctgtcc ggtctgcgta cctacatctt cgcgcactct 721 acctcccgca tcgacgttga actgggtgcg aagctgttcc gtcacctgct ggcactgcct 781 atcagctact tcgaatctcg tcgtgtgggc gatactgtgg cccgtgttcg tgaactggat 841 cagatccgca actttctgac cggccaggcc ctgaccagcg ttctggatct gctgttcagc 901 tttatcttct tcgcagtaat gtggtactac agcccgaagc tgactctggt gattctgttc 961 tctctgccgt gttacgcagc ttggtccgtc ttcatctctc caatcctgcg tcgccgtctg 1021 gacgacaaat ttagccgtaa tgcggataat cagtccttcc tggtcgaatc tgtgactgct 1081 atcaacacta tcaaagccat ggcggtttct ccacagatga ctaacatctg ggataagcag 1141 ctggcaggtt acgttgctgc gggcttcaaa gtgaccgttc tggcaaccat tggtcaacag 1201 ggcattcagc tgattcagaa aaccgtgatg atcattaacc tgtggctggg tgcgcacctg 1261 gtcatcagcg gtgacctgtc cattggccag ctgatcgctt tcaacatgct ggcgggtcaa 1321 attgttgccc cagtaatccg tctggcccag atctggcagg acttccagca ggtcggtatt 1381 tctgtgaccc gcctgggtga cgtcctgaac tctccgaccg aaagctacca cggtaaactg 1441 gctctgccgg agatcaacgg cgatattact ttccgtaaca tccgttttcg ttacaaaccg 1501 gattccccgg taatcctgga taacatcaat ctgtctatca aacagggcga agtcatcggc 1561 atcgtgggcc gtagcggcag cggcaagtcc actctgacta aactgattca gcgcttttac 1621 attccagaaa acggtcaggt tctgattgac ggtcacgacc tggctctggc ggacccgaac 1681 tggctgcgtc gtcaggtggg cgtagtgctg caagataacg tgctgctgaa ccgtagcatc 1741 atcgataaca tcagcctggc taacccgggc atgtccgtgg aaaaagttat ctatgctgcg 1801 aaactggcag gcgcgcacga tttcatctct gaactgcgtg aaggctacaa caccatcgta 1861 ggcgaacagg gcgctggcct gtccggcggt caacgtcagc gtatcgctat tgcacgtgca 1921 ctggtgaaca acccgaaaat cctgatcttc gacgaggcca cttctgctct ggattacgaa 1981 tctgaacata ttatcatgcg taatatgcac aagatctgca aaggtcgcac cgtaatcatc 2041 atcgcccacc gtctgagcac cgtaaagaac gccgaccgca tcatcgtgat ggaaaaaggt 2101 aagatcgttg aacagggtaa acataaagag ctgctgtccg aaccggaatc tctgtattct 2161 tatctgtacc agctgcaatc tgattaatac tagagattaa agaggagaat actagatgaa 2221 aacttggctg atgggcttct ctgagttcct gctgcgttat aagctggtat ggagcgaaac 2281 ttggaaaatc cgtaagcaac tggatacccc ggtacgtgaa aaagacgaaa acgaatttct 2341 gccagcacat ctggaactga ttgagacgcc ggtttctcgc cgtccacgtc tggttgctta 2401 cttcattatg ggcttcctgg ttattgctgt aatcctgtct gttctgggtc aggtggaaat 2461 cgttgcgacc gcgaatggta aactgaccct gtccggtcgt tctaaagaaa tcaaaccgat 2521 cgaaaactcc atcgttaaag agattatcgt taaagaaggt gaatccgtac gcaaaggcga 2581 tgttctgctg aaactgaccg cactgggcgc agaagcggac actctgaaaa ctcagtcctc 2641 tctgctgcaa acccgtctgg aacagacccg ttaccaaatc ctgagccgtt ctattgaact 2701 gaacaaactg ccggaactga aactgccgga tgaaccatac ttccagaacg taagcgagga 2761 ggaagttctg cgcctgactt ccctgatcaa agagcagttc tctacttggc agaaccagaa 2821 ataccaaaag gaactgaacc tggacaaaaa gcgtgcggag cgcctgacta tcctggcccg 2881 catcaaccgt tacgagaacc tgtctcgtgt tgaaaaatct cgcctggacg acttccgtag 2941 cctgctgcac aaacaggcca tcgcaaaaca cgctgtgctg gaacaggaaa ataaatacgt 3001 ggaagctgcc aacgaactgc gtgtatataa aagccagctg gagcagatcg agagcgaaat 3061 cctgtctgcg aaggaggagt accagctggt gacgcagctg ttcaaaaatg aaatcctgga 3121 caaactgcgt caaaccacgg actctatcga actgctgacc ctggaactgg agaaaaacga 3181 ggagcgccag caggccagcg tcatccgcgc tccggtatct ggtaaagttc agcaactgaa 3241 agttcacacc gaaggtggcg ttgttaccac cgcagaaacc ctgatggtta ttgtgccgga 3301 agatgacacc ctggaagtta ccgctctggt gcagaacaaa gatatcggtt tcatcaacgt 3361 cggtcagaac gcgatcatca aagtagaggc tttcccgtac actcgttacg gctacctggt 3421 gggcaaggtc aaaaacatca acctggacgc tatcgaagat cagaaactgg gcctggtgtt 3481 caacgtgatc gttagcgtag aagagaacga tctgtctacc ggtaacaaac acattccact 3541 gtcctccggc atggcggtta ctgctgaaat caaaaccggt atgcgtagcg tgatctccta 3601 tctgctgtct ccgctggaag aaagcgtcac cgaatctctg cacgaacgct aatactagag 3661 aaaaaaaaac cccgcccctg acagggcggg gtttttttt //