BBa_M1007
type: ComponentDefinition
persistent identity: BBa M1007
display id: BBa_M1007
version: 1
was generated by: iGEM to SBOL conversion
description: Complete Phasin Secretion Construct
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: See subparts
owned by: myers
owned by: james
mutable description: This construct contains all of the components necessary for the secretion of phasin through type I secretion. Phasin is fused to the HlyA secretion signal peptide under high constitutive expression. The construct also contains the HlyB and HlyD membrane proteins that interact with the HlyA tag and allow secretion.
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1006
component: BBa_K208006
component: BBa_K208001
component: BBa_M1005
sequence annotation: BBa_M1005
sequence annotation: BBa_K208001
sequence annotation: BBa_K208006
sequence annotation: BBa_M1006
sequence: https://synbiohub.org/public/igem/BBa_M1007_sequence/1

BBa_M1006
type: Component
persistent identity: component2116046
display id: component2116046
version: 1
top level: BBa_M1007
definition: BBa_M1006
access: public

BBa_K208006
type: Component
persistent identity: component2116028
display id: component2116028
version: 1
top level: BBa_M1007
definition: BBa_K208006
access: public

BBa_K208001
type: Component
persistent identity: component2116025
display id: component2116025
version: 1
top level: BBa_M1007
definition: BBa_K208001
access: public

BBa_M1005
type: Component
persistent identity: component2116022
display id: component2116022
version: 1
top level: BBa_M1007
definition: BBa_M1005
access: public

BBa_M1005
type: SequenceAnnotation
persistent identity: annotation2116022
display id: annotation2116022
version: 1
top level: BBa_M1007
location: https://synbiohub.org/public/igem/BBa_M1007/annotation2116022/range2116022/1
component: BBa_M1005

https://synbiohub.org/public/igem/BBa_M1007/annotation2116022/range2116022/1
type: Range
persistent identity: range2116022
display id: range2116022
version: 1
top level: BBa_M1007
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K208001
type: SequenceAnnotation
persistent identity: annotation2116025
display id: annotation2116025
version: 1
top level: BBa_M1007
location: https://synbiohub.org/public/igem/BBa_M1007/annotation2116025/range2116025/1
component: BBa_K208001

https://synbiohub.org/public/igem/BBa_M1007/annotation2116025/range2116025/1
type: Range
persistent identity: range2116025
display id: range2116025
version: 1
top level: BBa_M1007
direction: direction/reverse
start: 64
end: 642
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2116028
display id: annotation2116028
version: 1
top level: BBa_M1007
location: https://synbiohub.org/public/igem/BBa_M1007/annotation2116028/range2116028/1
component: BBa_K208006

https://synbiohub.org/public/igem/BBa_M1007/annotation2116028/range2116028/1
type: Range
persistent identity: range2116028
display id: range2116028
version: 1
top level: BBa_M1007
direction: direction/reverse
start: 651
end: 836
orientation: inline

BBa_M1006
type: SequenceAnnotation
persistent identity: annotation2116046
display id: annotation2116046
version: 1
top level: BBa_M1007
location: https://synbiohub.org/public/igem/BBa_M1007/annotation2116046/range2116046/1
component: BBa_M1006

https://synbiohub.org/public/igem/BBa_M1007/annotation2116046/range2116046/1
type: Range
persistent identity: range2116046
display id: range2116046
version: 1
top level: BBa_M1007
direction: direction/reverse
start: 845
end: 4543
orientation: inline

BBa_K208001
type: ComponentDefinition
persistent identity: BBa K208001
display id: BBa_K208001
version: 1
was generated by: iGEM to SBOL conversion
description: Phasin (PhaP)
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: G
owned by: james
owned by: myers
mutable description: G
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 2425
owning group id: 303
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: G
star: false
creator: Elisabeth Linton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start
sequence annotation: Phasin
sequence: https://synbiohub.org/public/igem/BBa_K208001_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2062218
display id: annotation2062218
version: 1
top level: BBa_K208001
location: https://synbiohub.org/public/igem/BBa_K208001/annotation2062218/range2062218/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K208001/annotation2062218/range2062218/1
type: Range
persistent identity: range2062218
display id: range2062218
version: 1
top level: BBa_K208001
direction: direction/forward
start: 1
end: 3
orientation: inline

Phasin
type: SequenceAnnotation
persistent identity: annotation2062219
display id: annotation2062219
version: 1
top level: BBa_K208001
location: https://synbiohub.org/public/igem/BBa_K208001/annotation2062219/range2062219/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K208001/annotation2062219/range2062219/1
type: Range
persistent identity: range2062219
display id: range2062219
version: 1
top level: BBa_K208001
direction: direction/forward
start: 1
end: 579
orientation: inline

BBa_K208006
type: ComponentDefinition
persistent identity: BBa K208006
display id: BBa_K208006
version: 1
was generated by: iGEM to SBOL conversion
description: HlyA Signal Peptide - Silver Fusion Compatible
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: Synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: HlyA
discontinued: false
dominant: true
experience: experience/None
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Silver Fusion
star: false
creator: USU iGEM 2009
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: HlyA
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K208006_sequence/1

HlyA
type: SequenceAnnotation
persistent identity: annotation2034624
display id: annotation2034624
version: 1
top level: BBa_K208006
location: https://synbiohub.org/public/igem/BBa_K208006/annotation2034624/range2034624/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K208006/annotation2034624/range2034624/1
type: Range
persistent identity: range2034624
display id: range2034624
version: 1
top level: BBa_K208006
direction: direction/forward
start: 1
end: 186
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2062223
display id: annotation2062223
version: 1
top level: BBa_K208006
location: https://synbiohub.org/public/igem/BBa_K208006/annotation2062223/range2062223/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K208006/annotation2062223/range2062223/1
type: Range
persistent identity: range2062223
display id: range2062223
version: 1
top level: BBa_K208006
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_M1005
type: ComponentDefinition
persistent identity: BBa M1005
display id: BBa_M1005
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter+RBS
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Combination of registry parts.
owned by: james
owned by: myers
mutable description: Constitutive promoter with high transcriptional levels reported. RBS has high level of translation.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23100
component: BBa_B0035
sequence annotation: BBa_J23100
sequence annotation: BBa_B0035
sequence: https://synbiohub.org/public/igem/BBa_M1005_sequence/1

BBa_J23100
type: Component
persistent identity: component2115955
display id: component2115955
version: 1
top level: BBa_M1005
definition: BBa_J23100
access: public

BBa_B0035
type: Component
persistent identity: component2115956
display id: component2115956
version: 1
top level: BBa_M1005
definition: BBa_B0035
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2115955
display id: annotation2115955
version: 1
top level: BBa_M1005
location: https://synbiohub.org/public/igem/BBa_M1005/annotation2115955/range2115955/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_M1005/annotation2115955/range2115955/1
type: Range
persistent identity: range2115955
display id: range2115955
version: 1
top level: BBa_M1005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2115956
display id: annotation2115956
version: 1
top level: BBa_M1005
location: https://synbiohub.org/public/igem/BBa_M1005/annotation2115956/range2115956/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1005/annotation2115956/range2115956/1
type: Range
persistent identity: range2115956
display id: range2115956
version: 1
top level: BBa_M1005
direction: direction/reverse
start: 44
end: 57
orientation: inline

BBa_M1006
type: ComponentDefinition
persistent identity: BBa M1006
display id: BBa_M1006
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, RBS, HlyB, RBS, HlyD, Term
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: See subparts
owned by: james
owned by: myers
mutable description: Composite part for high levels of production of the HlyB and HlyD membrane proteins
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0035
component: BBa_M1003
component: BBa_M1005
component: BBa_B1006
component: BBa_M1004
sequence annotation: BBa_M1005
sequence annotation: BBa_M1003
sequence annotation: BBa_B0035
sequence annotation: BBa_M1004
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_M1006_sequence/1

BBa_B0035
type: Component
persistent identity: component2116004
display id: component2116004
version: 1
top level: BBa_M1006
definition: BBa_B0035
access: public

BBa_M1003
type: Component
persistent identity: component2116003
display id: component2116003
version: 1
top level: BBa_M1006
definition: BBa_M1003
access: public

BBa_M1005
type: Component
persistent identity: component2115999
display id: component2115999
version: 1
top level: BBa_M1006
definition: BBa_M1005
access: public

BBa_B1006
type: Component
persistent identity: component2116013
display id: component2116013
version: 1
top level: BBa_M1006
definition: BBa_B1006
access: public

BBa_M1004
type: Component
persistent identity: component2116008
display id: component2116008
version: 1
top level: BBa_M1006
definition: BBa_M1004
access: public

BBa_M1005
type: SequenceAnnotation
persistent identity: annotation2115999
display id: annotation2115999
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2115999/range2115999/1
component: BBa_M1005

https://synbiohub.org/public/igem/BBa_M1006/annotation2115999/range2115999/1
type: Range
persistent identity: range2115999
display id: range2115999
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2116003
display id: annotation2116003
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116003/range2116003/1
component: BBa_M1003

https://synbiohub.org/public/igem/BBa_M1006/annotation2116003/range2116003/1
type: Range
persistent identity: range2116003
display id: range2116003
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 64
end: 2187
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2116004
display id: annotation2116004
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116004/range2116004/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1006/annotation2116004/range2116004/1
type: Range
persistent identity: range2116004
display id: range2116004
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 2196
end: 2209
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2116008
display id: annotation2116008
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116008/range2116008/1
component: BBa_M1004

https://synbiohub.org/public/igem/BBa_M1006/annotation2116008/range2116008/1
type: Range
persistent identity: range2116008
display id: range2116008
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 2216
end: 3652
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2116013
display id: annotation2116013
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116013/range2116013/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_M1006/annotation2116013/range2116013/1
type: Range
persistent identity: range2116013
display id: range2116013
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 3661
end: 3699
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_M1003
type: ComponentDefinition
persistent identity: BBa M1003
display id: BBa_M1003
version: 1
was generated by: iGEM to SBOL conversion
description: HlyB membrane protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UT189
owned by: myers
owned by: james
mutable description: Membrane protein involved in type I secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115949
display id: annotation2115949
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
type: Range
persistent identity: range2115949
display id: range2115949
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2115951
display id: annotation2115951
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
type: Range
persistent identity: range2115951
display id: range2115951
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 2121
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115950
display id: annotation2115950
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
type: Range
persistent identity: range2115950
display id: range2115950
version: 1
top level: BBa_M1003
direction: direction/forward
start: 2122
end: 2124
orientation: inline

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: iGEM to SBOL conversion
description: HlyD Membrane Protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyD
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 1434
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1435
end: 1437
orientation: inline

https://synbiohub.org/public/igem/BBa_M1007_sequence/1
type: Sequence
persistent identity: BBa M1007 sequence
display id: BBa_M1007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgtggttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208001_sequence/1
type: Sequence
persistent identity: BBa K208001 sequence
display id: BBa_K208001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208006_sequence/1
type: Sequence
persistent identity: BBa K208006 sequence
display id: BBa_K208006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1005_sequence/1
type: Sequence
persistent identity: BBa M1005 sequence
display id: BBa_M1005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1006_sequence/1
type: Sequence
persistent identity: BBa M1006 sequence
display id: BBa_M1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgtggttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1003_sequence/1
type: Sequence
persistent identity: BBa M1003 sequence
display id: BBa_M1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1004_sequence/1
type: Sequence
persistent identity: BBa M1004 sequence
display id: BBa_M1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z