This construct contains all of the components necessary for the secretion of phasin through type I secretion. Phasin is fused to the HlyA secretion signal peptide under high constitutive expression. The construct also contains the HlyB and HlyD membrane proteins that interact with the HlyA tag and allow secretion.
HlyA Signal Peptide - Silver Fusion Compatible
Constitutive promoter with high transcriptional levels reported. RBS has high level of translation.
Composite part for high levels of production of the HlyB and HlyD membrane proteins
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
Membrane protein involved in type I secretion.
Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
Conversion of the iGEM parts registry to SBOL2.1