BBa_M10086
1
BBa_M10086
{˂CPG_L2!}
2009-04-30T11:00:00Z
2015-05-08T01:13:51Z
E. coli MG1655 genomic DNA
Circularly permutated OmpG with a backbone opening in loop 2 of the native protein.
Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment. <br>
CPG_L2 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {<CPG_L2!} and should be functionally displayed on the outer membrane of E.coli.
false
false
_271_
0
4745
271
Not in stock
true
Needed to design appropriate linkers (to fuse the N and C termini of the native protien). Used 6aa linker (CDS: GGATCTAAGTCTCGTCGC) from PMID: 18480093. <br>
The opening was created in loop 2 (aa 59-60) because the residues of the mature protein are presumably disordered because they are not visible in electron density maps. <br>
false
Jennifer Brophy
annotation2002600
1
Native C termini
range2002600
1
1
663
annotation2002598
1
Linker
range2002598
1
664
681
annotation2002599
1
Native N termini
range2002599
1
682
861
BBa_M10086_sequence
1
ggtaaacgtggaacgtggtttgatcgcccggagctggaggtgcattatcagttcctcgaaaacgatgatttcagtttcggcctgaccggcggtttccgtaattatggttatcactacgttgatgaaccgggtaaagacacggcgaatatgcagcgctggaaaatcgcgccagactgggatgtgaaactgactgacgatttacgtttcaacggttggttgtcgatgtataaatttgccaacgatctgaacactaccggttacgctgatacccgtgtcgaaacggaaacaggtctgcaatataccttcaacgaaacggttgccttgcgagtgaactattatcttgagcgcggcttcaatatggacgacagccgcaataacggtgagttttccacgcaagaaattcgcgtctatttgccgctgacgctcggcaaccactcggtgacgccgtatacgcgcattgggctggatcgctggagtaactgggactggcaggatgatattgaacgtgaaggccatgattttaaccgtgtaggtttattttacggttatgatttccagaacggactttccgtttcgctggaatacgcgtttgagtggcaggatcacgacgaaggcgacagtgataaattccattatgcaggtgtcggcgtaaattactcgttaggatctaagtctcgtcgcgaggaaaggaacgactggcactttaatatcggcgcgatgtacgaaatagaaaacgtcgagggttatggcgaagatatggatgggctggcggagccttcagtctattttaatgccgccaacgggccgtggagaattgctctggcctattatcgggaagggccggtagattatagcgcgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z