BBa_M10090
1
BBa_M10090
{˂Chal˃}
2009-05-03T11:00:00Z
2015-05-08T01:13:51Z
Construction of {<Chal>} basic part from pBca9145-Jtk2031
PCR ca998 and Odmd003R on pBca9145-Jtk2031 (510bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10051 {<Chal>}
----------------------------
ca998 Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag
Odmd003R Reverse Oligo for Chal Lectin w/o Stop Codon ccaaaGGATCCctcgctgtcgtcctctcgta
For background you should read PMID: 15039097. ChaI is a lectin (a sugar-binding protein) that binds to polysialic acid which is the outer shell composition of E. coli expressing the K1 capsule.
false
false
_271_
0
2632
271
Not in stock
false
Part designed during Bio140L at UC Berkeley during Spring 2009
false
Douglas Densmore
BBa_M10090_sequence
1
cagatcgtcgaaccaactgtggccttcacgactgtgcttgataaaaaccttgttcttgaaaacggcgacacactgatcccgaacaagattctcatcaactacggcggcggctacaacgataagactgggatcttcacggcgcccaagagtggaatctaccacttgggagtgcatgcacagacctcccttcaatccaacttgtggctagctctctaccacaatgacaattacgtcttttccatatacggacgtcaaactgaatactcggatgggggagcaaacgcggccattctgccactgaagaaaggcgacaaggtccacgtgaaagctcgcgataaatcatcccttctgggcagacctgacaacatctacaccaccttcactggcttccgcctgggtccgctacgagaggacgacagcgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z