LOCUS BBa_M1014 6805 bp DNA linear 26-Nov-2024 DEFINITION Complete construct for phasin secretion together with zinc finger binding sites ACCESSION BBa_M1014 VERSION BBa_M1014.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..57 /label=BBa_B0035 misc_feature 1..57 /label=BBa_M1005 misc_feature 64..66 /label=Start Codon CDS 64..639 /label=Phasin CDS 646..825 /label=HlyA misc_feature 826..831 /label=Double Stop CDS 64..831 /label=BBa_M1010 RBS 840..853 /label=BBa_B0035 misc_feature 860..862 /label=Start Codon misc_feature 2981..2983 /label=Stop Codon CDS 860..2980 /label=HlyB CDS 860..2983 /label=BBa_M1003 RBS 2992..3005 /label=BBa_B0035 misc_feature 3012..3014 /label=Start Codon CDS 3012..4445 /label=HlyD misc_feature 4446..4448 /label=Stop Codon CDS 3012..4448 /label=BBa_M1004 RBS 4457..4470 /label=BBa_B0035 misc_feature 4477..4479 /label=Start misc_feature 5992..5994 /label=Stop CDS 4477..5991 /label=TolC CDS 4477..5994 /label=BBa_M1028 misc_feature 6003..6041 /label=B1006 stem_loop 6012..6033 /label=modified thr terminator polyA_site 6034..6041 /label=PolyA polyA_site 6003..6011 /label=PolyA terminator 6003..6041 /label=BBa_B1006 misc_feature 840..6041 /label=BBa_M1027 misc_feature 1..6041 /label=BBa_M1013 misc_feature 6050..6805 /label=BBa_M1026 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaatac 61 tagatgatcc tcaccccgga acaagttgca gcagcgcaaa aggccaacct cgaaacgctg 121 ttcggcctga ccaccaaggc gtttgaaggc gtcgaaaagc tcgtcgagct gaaccttcag 181 gtcgtcaaga cttcgttcgc agaaggcgtt gacaacgcca agaaggcgct gtcggccaag 241 gacgcacagg aactgctggc catccaggcc gcagccgtgc agccggttgc cgaaaagacc 301 ctggcctaca cccgccacct gtatgaaatc gcttcggaaa cccagagcga gttcaccaag 361 gtagccgagg ctcaactggc cgaaggctcg aagaacgtgc aagcgctggt cgagaacctc 421 gccaagaacg ccccggccgg ttcggaatcg accgtggcca tcgtgaagtc ggcgatctcc 481 gctgccaaca acgcctacga gtcggtgcag aaggcgacca agcaagcggt cgaaatcgct 541 gaaaccaact tccaggctgc ggctacggct gccaccaagg ctgcccagca agccagcgcc 601 acggcccgta cggccacggc aaagaagacg acggctgcca ctagattagc ctatggaagt 661 cagggtgatc ttaatccatt aattaatgaa atcagcaaaa tcatttcagc agcaggtagc 721 ttcgatgtta aagaggaaag aaccgcagct tctttattgc agttgtccgg taatgccagt 781 gatttttcat atggacggaa ctcaataacc ctgaccacat cagcataata atactagaga 841 ttaaagagga gaatactaga tggattcctg tcacaagatt gattacggcc tgtatgcact 901 ggaaatcctg gcacaatatc ataacgtctc cgtcaacccg gaggaaatta aacatcgctt 961 cgatactgac ggcactggcc tgggtctgac cagctggctg ctggcagcga agtccctgga 1021 gctgaaggta aagcaagtga aaaagacgat tgatcgtctg aacttcatca gcctgccggc 1081 actggtttgg cgtgaagatg gccgtcactt catcctgacg aaagtgtcca aagaagcgaa 1141 ccgctacctg atctttgatc tggagcagcg caacccgcgc gtactggaac agagcgagtt 1201 cgaggctctg tatcagggcc acatcattct gattgcatct cgttcttctg ttgcaggtaa 1261 actggcgaaa ttcgacttca cctggttcat tccggctatt atcaagtacc gtcgtatttt 1321 catcgaaacc ctggttgtat ctgttttcct gcaactgttc gctctgatta ctccgctgtt 1381 ctttcaggtt gtcatggaca aagttctggt tcaccgtggc ttttccaccc tgaacgtcat 1441 tactgttgcg ctgagcgttg ttgttgtgtt cgaaatcatt ctgtccggtc tgcgtaccta 1501 catcttcgcg cactctacct cccgcatcga cgttgaactg ggtgcgaagc tgttccgtca 1561 cctgctggca ctgcctatca gctacttcga atctcgtcgt gtgggcgata ctgtggcccg 1621 tgttcgtgaa ctggatcaga tccgcaactt tctgaccggc caggccctga ccagcgttct 1681 ggatctgctg ttcagcttta tcttcttcgc agtaatgtgg tactacagcc cgaagctgac 1741 tctggtgatt ctgttctctc tgccgtgtta cgcagcttgg tccgtcttca tctctccaat 1801 cctgcgtcgc cgtctggacg acaaatttag ccgtaatgcg gataatcagt ccttcctggt 1861 cgaatctgtg actgctatca acactatcaa agccatggcg gtttctccac agatgactaa 1921 catctgggat aagcagctgg caggttacgt tgctgcgggc ttcaaagtga ccgttctggc 1981 aaccattggt caacagggca ttcagctgat tcagaaaacc gtgatgatca ttaacctgtg 2041 gctgggtgcg cacctggtca tcagcggtga cctgtccatt ggccagctga tcgctttcaa 2101 catgctggcg ggtcaaattg ttgccccagt aatccgtctg gcccagatct ggcaggactt 2161 ccagcaggtc ggtatttctg tgacccgcct gggtgacgtc ctgaactctc cgaccgaaag 2221 ctaccacggt aaactggctc tgccggagat caacggcgat attactttcc gtaacatccg 2281 ttttcgttac aaaccggatt ccccggtaat cctggataac atcaatctgt ctatcaaaca 2341 gggcgaagtc atcggcatcg tgggccgtag cggcagcggc aagtccactc tgactaaact 2401 gattcagcgc ttttacattc cagaaaacgg tcaggttctg attgacggtc acgacctggc 2461 tctggcggac ccgaactggc tgcgtcgtca ggtgggcgta gtgctgcaag ataacgtgct 2521 gctgaaccgt agcatcatcg ataacatcag cctggctaac ccgggcatgt ccgtggaaaa 2581 agttatctat gctgcgaaac tggcaggcgc gcacgatttc atctctgaac tgcgtgaagg 2641 ctacaacacc atcgtaggcg aacagggcgc tggcctgtcc ggcggtcaac gtcagcgtat 2701 cgctattgca cgtgcactgg tgaacaaccc gaaaatcctg atcttcgacg aggccacttc 2761 tgctctggat tacgaatctg aacatattat catgcgtaat atgcacaaga tctgcaaagg 2821 tcgcaccgta atcatcatcg cccaccgtct gagcaccgta aagaacgccg accgcatcat 2881 cgtgatggaa aaaggtaaga tcgttgaaca gggtaaacat aaagagctgc tgtccgaacc 2941 ggaatctctg tattcttatc tgtaccagct gcaatctgat taatactaga gattaaagag 3001 gagaatacta gatgaaaact tggctgatgg gcttctctga gttcctgctg cgttataagc 3061 tggtatggag cgaaacttgg aaaatccgta agcaactgga taccccggta cgtgaaaaag 3121 acgaaaacga atttctgcca gcacatctgg aactgattga gacgccggtt tctcgccgtc 3181 cacgtctggt tgcttacttc attatgggct tcctggttat tgctgtaatc ctgtctgttc 3241 tgggtcaggt ggaaatcgtt gcgaccgcga atggtaaact gaccctgtcc ggtcgttcta 3301 aagaaatcaa accgatcgaa aactccatcg ttaaagagat tatcgttaaa gaaggtgaat 3361 ccgtacgcaa aggcgatgtt ctgctgaaac tgaccgcact gggcgcagaa gcggacactc 3421 tgaaaactca gtcctctctg ctgcaaaccc gtctggaaca gacccgttac caaatcctga 3481 gccgttctat tgaactgaac aaactgccgg aactgaaact gccggatgaa ccatacttcc 3541 agaacgtaag cgaggaggaa gttctgcgcc tgacttccct gatcaaagag cagttctcta 3601 cttggcagaa ccagaaatac caaaaggaac tgaacctgga caaaaagcgt gcggagcgcc 3661 tgactatcct ggcccgcatc aaccgttacg agaacctgtc tcgtgttgaa aaatctcgcc 3721 tggacgactt ccgtagcctg ctgcacaaac aggccatcgc aaaacacgct gtgctggaac 3781 aggaaaataa atacgtggaa gctgccaacg aactgcgtgt atataaaagc cagctggagc 3841 agatcgagag cgaaatcctg tctgcgaagg aggagtacca gctggtgacg cagctgttca 3901 aaaatgaaat cctggacaaa ctgcgtcaaa ccacggactc tatcgaactg ctgaccctgg 3961 aactggagaa aaacgaggag cgccagcagg ccagcgtcat ccgcgctccg gtatctggta 4021 aagttcagca actgaaagtt cacaccgaag gtggcgttgt taccaccgca gaaaccctga 4081 tggttattgt gccggaagat gacaccctgg aagttaccgc tctggtgcag aacaaagata 4141 tcggtttcat caacgtcggt cagaacgcga tcatcaaagt agaggctttc ccgtacactc 4201 gttacggcta cctggtgggc aaggtcaaaa acatcaacct ggacgctatc gaagatcaga 4261 aactgggcct ggtgttcaac gtgatcgtta gcgtagaaga gaacgatctg tctaccggta 4321 acaaacacat tccactgtcc tccggcatgg cggttactgc tgaaatcaaa accggtatgc 4381 gtagcgtgat ctcctatctg ctgtctccgc tggaagaaag cgtcaccgaa tctctgcacg 4441 aacgctaata ctagagatta aagaggagaa tactagatga ttgccctgaa cactgcctcg 4501 ccacagggga tgcagatgaa gaagctcctg ccgatcctga tcgggctaag tctttccggg 4561 ttcagctcac tttcacaagc cgagaatctt atgcaagtat atcagcaggc acggctgtcg 4621 aatccagagc ttcgtaaatc agcggccgat cgagatgctg cgttcgagaa aattaatgag 4681 gcccggtcac cgctgttacc acagctgggt ctcggcgcgg attatacata cagcaacggc 4741 taccgtgatg cgaatggaat aaatagtaac gcaaccagcg caagcttaca actgacacag 4801 tcgatcttcg atatgtcgaa atggcgcgca ctaacgcttc aggaaaaagc agccgggatc 4861 caagatgtaa cttatcagac cgaccagcaa acattgattt tgaatactgc aaccgcttac 4921 tttaacgttc tgaatgccat cgacgtgctg agctacaccc aggcacaaaa ggaagccatt 4981 tatcgtcaac ttgaccagac tacgcagcgg tttaacgttg ggttagtagc aatcaccgat 5041 gttcaaaatg cgcgtgcgca gtatgatacc gttctggcca atgaggtcac cgcacgcaac 5101 aatcttgata atgcggttga acagttacgt cagattaccg ggaactatta tcccgagctg 5161 gcagcgctga atgtggaaaa ttttaaaact gacaaacctc aacctgtcaa taccctgctc 5221 aaggaagccg agaaacgcaa cctgtctcta ttgcaagctc gcctgtctca agacctggct 5281 cgggagcaaa tacgccaggc acaggacgga catctgccca cactggacct gaccgcaagt 5341 actgggatat ccgacaccag ttactctgga tcaaaaactc gtggcgccgc cggaacacaa 5401 tacgatgact caaatatggg ccagaacaag gttggcctgt ccttcagttt accgatctac 5461 cagggcggga tggtgaatag tcaagtaaaa caggcgcagt ataactttgt cggtgcatca 5521 gagcaactgg aatcagctca ccgtagcgtc gtacagactg tccggagtag ctttaacaac 5581 attaacgcga gtatctcatc aatcaatgct tacaagcaag cagtggtttc tgcgcagtct 5641 tctttggatg ccatggaagc aggctattca gtgggtactc gcacgattgt tgatgttctc 5701 gacgctacca caactctcta caacgcgaaa caggaattag cgaatgcccg ttataactac 5761 ttaattaacc aactgaatat caaatctgcc ctaggtactc tgaatgagca agacttgctt 5821 gcgttaaata acgccctgtc caaacctgtc tcaaccaatc ctgagaacgt agcacctcag 5881 acccctgaac agaacgccat tgcggacggt tacgcccctg acagtcctgc gcctgtggtc 5941 cagcagacgt cagcgcgcac taccacttca aacgggcata atccgttccg caattactag 6001 agaaaaaaaa accccgcccc tgacagggcg gggttttttt ttactagagt ttgatgctgc 6061 atttgcgtgg tttgtttgga tgttttttga tgctgcattt gcgtggtttg tttggatgtt 6121 ttttgatgct gcatttgcgt ggtttgtttg gatgtttttt gatgctgcat ttgcgtggtt 6181 tgtttggatg ttttttgatg ctgcatttgc gtggtttgtt tggatgtttt ttgatgctgc 6241 atttgcgtgg tttgtttgga tgttttttga tgctgcattt gcgtggtttg tttggatgtt 6301 ttttgatgct gcatttgcgt ggtttgtttg gatgtttttt gatgctgcat ttgcgtggtt 6361 tgtttggatg ttttttgatg ctgcatttgc gtggtttgtt tggatgtttt ttgatgctgc 6421 atttgcgtgg tttgtttgga tgttttttga tgctgcattt gcgtggtttg tttggatgtt 6481 ttttgatgct gcatttgcgt ggtttgtttg gatgtttttt gatgctgcat ttgcgtggtt 6541 tgtttggatg ttttttgatg ctgcatttgc gtggtttgtt tggatgtttt ttgatgctgc 6601 atttgcgtgg tttgtttgga tgttttttga tgctgcattt gcgtggtttg tttggatgtt 6661 ttttgatgct gcatttgcgt ggtttgtttg gatgtttttt gatgctgcat ttgcgtggtt 6721 tgtttggatg ttttttgatg ctgcatttgc gtggtttgtt tggatgtttt ttgatgctgc 6781 atttgcgtgg tttgtttgga tgttt //