BBa_M1014
type: ComponentDefinition
persistent identity: BBa M1014
display id: BBa_M1014
version: 1
was generated by: iGEM to SBOL conversion
description: Complete construct for phasin secretion together with zinc finger binding sites
created: 2011-04-18T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: See Subparts
owned by: james
owned by: myers
mutable description: This construct is designed to exist in a plasmid separate from a plasmid coding for the zinc finger-fused enzymes involved in the PHB enzymatic pathway. This construct codes for phasin targeted for secretion by the Type I pathway as well as the Type I associated membrane proteins HlyB and HlyD. It also contains a repeated sequence that allows ordered binding of Zif268-HIV, Zif23-GCN 4, and "Blues" Zinc fingers.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1013
component: BBa_M1026
sequence annotation: BBa_M1013
sequence annotation: BBa_M1026
sequence: https://synbiohub.org/public/igem/BBa_M1014_sequence/1

BBa_M1013
type: Component
persistent identity: component2117678
display id: component2117678
version: 1
top level: BBa_M1014
definition: BBa_M1013
access: public

BBa_M1026
type: Component
persistent identity: component2117679
display id: component2117679
version: 1
top level: BBa_M1014
definition: BBa_M1026
access: public

BBa_M1013
type: SequenceAnnotation
persistent identity: annotation2117678
display id: annotation2117678
version: 1
top level: BBa_M1014
location: https://synbiohub.org/public/igem/BBa_M1014/annotation2117678/range2117678/1
component: BBa_M1013

https://synbiohub.org/public/igem/BBa_M1014/annotation2117678/range2117678/1
type: Range
persistent identity: range2117678
display id: range2117678
version: 1
top level: BBa_M1014
direction: direction/reverse
start: 1
end: 6041
orientation: inline

BBa_M1026
type: SequenceAnnotation
persistent identity: annotation2117679
display id: annotation2117679
version: 1
top level: BBa_M1014
location: https://synbiohub.org/public/igem/BBa_M1014/annotation2117679/range2117679/1
component: BBa_M1026

https://synbiohub.org/public/igem/BBa_M1014/annotation2117679/range2117679/1
type: Range
persistent identity: range2117679
display id: range2117679
version: 1
top level: BBa_M1014
direction: direction/reverse
start: 6050
end: 6805
orientation: inline

BBa_M1026
type: ComponentDefinition
persistent identity: BBa M1026
display id: BBa_M1026
version: 1
was generated by: iGEM to SBOL conversion
description: DNA Scaffold for Binding Domains
created: 2011-04-17T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Scaffold contain the DNA sequences where the zinc fingers HIVC GN23 and blue zinc finger bind.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 9096
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Misheka Wilson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M1026_sequence/1

BBa_M1013
type: ComponentDefinition
persistent identity: BBa M1013
display id: BBa_M1013
version: 1
was generated by: iGEM to SBOL conversion
description: Complete Construct for Type I Phasin Secretion
created: 2011-04-18T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: See Subparts
owned by: james
owned by: myers
mutable description: This construct codes for phasin targeted for secretion with the HlyA signal peptide. It also codes for the HlyB and HlyD membrane proteins necessary for type I secretion. Coding regions are controlled under high levels of constitutive expression.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1027
component: BBa_M1010
component: BBa_M1005
sequence annotation: BBa_M1005
sequence annotation: BBa_M1010
sequence annotation: BBa_M1027
sequence: https://synbiohub.org/public/igem/BBa_M1013_sequence/1

BBa_M1027
type: Component
persistent identity: component2117648
display id: component2117648
version: 1
top level: BBa_M1013
definition: BBa_M1027
access: public

BBa_M1010
type: Component
persistent identity: component2117627
display id: component2117627
version: 1
top level: BBa_M1013
definition: BBa_M1010
access: public

BBa_M1005
type: Component
persistent identity: component2117622
display id: component2117622
version: 1
top level: BBa_M1013
definition: BBa_M1005
access: public

BBa_M1005
type: SequenceAnnotation
persistent identity: annotation2117622
display id: annotation2117622
version: 1
top level: BBa_M1013
location: https://synbiohub.org/public/igem/BBa_M1013/annotation2117622/range2117622/1
component: BBa_M1005

https://synbiohub.org/public/igem/BBa_M1013/annotation2117622/range2117622/1
type: Range
persistent identity: range2117622
display id: range2117622
version: 1
top level: BBa_M1013
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_M1010
type: SequenceAnnotation
persistent identity: annotation2117627
display id: annotation2117627
version: 1
top level: BBa_M1013
location: https://synbiohub.org/public/igem/BBa_M1013/annotation2117627/range2117627/1
component: BBa_M1010

https://synbiohub.org/public/igem/BBa_M1013/annotation2117627/range2117627/1
type: Range
persistent identity: range2117627
display id: range2117627
version: 1
top level: BBa_M1013
direction: direction/reverse
start: 64
end: 831
orientation: inline

BBa_M1027
type: SequenceAnnotation
persistent identity: annotation2117648
display id: annotation2117648
version: 1
top level: BBa_M1013
location: https://synbiohub.org/public/igem/BBa_M1013/annotation2117648/range2117648/1
component: BBa_M1027

https://synbiohub.org/public/igem/BBa_M1013/annotation2117648/range2117648/1
type: Range
persistent identity: range2117648
display id: range2117648
version: 1
top level: BBa_M1013
direction: direction/reverse
start: 840
end: 6041
orientation: inline

BBa_M1005
type: ComponentDefinition
persistent identity: BBa M1005
display id: BBa_M1005
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter+RBS
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Combination of registry parts.
owned by: james
owned by: myers
mutable description: Constitutive promoter with high transcriptional levels reported. RBS has high level of translation.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_B0035
sequence annotation: BBa_J23100
sequence annotation: BBa_B0035
sequence: https://synbiohub.org/public/igem/BBa_M1005_sequence/1

BBa_J23100
type: Component
persistent identity: component2115955
display id: component2115955
version: 1
top level: BBa_M1005
definition: BBa_J23100
access: public

BBa_B0035
type: Component
persistent identity: component2115956
display id: component2115956
version: 1
top level: BBa_M1005
definition: BBa_B0035
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2115955
display id: annotation2115955
version: 1
top level: BBa_M1005
location: https://synbiohub.org/public/igem/BBa_M1005/annotation2115955/range2115955/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_M1005/annotation2115955/range2115955/1
type: Range
persistent identity: range2115955
display id: range2115955
version: 1
top level: BBa_M1005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2115956
display id: annotation2115956
version: 1
top level: BBa_M1005
location: https://synbiohub.org/public/igem/BBa_M1005/annotation2115956/range2115956/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1005/annotation2115956/range2115956/1
type: Range
persistent identity: range2115956
display id: range2115956
version: 1
top level: BBa_M1005
direction: direction/reverse
start: 44
end: 57
orientation: inline

BBa_M1010
type: ComponentDefinition
persistent identity: BBa M1010
display id: BBa_M1010
version: 1
was generated by: iGEM to SBOL conversion
description: Phasin HlyA Silver Fusion
created: 2011-04-18T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: See BBa_K208029
owned by: james
owned by: myers
mutable description: This is a modification of the part BBa_K208029 which already exists in the registry. This part contains the scar that corresponds to Silver Fusion.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Phasin
sequence annotation: HlyA
sequence annotation: Double Stop
sequence: https://synbiohub.org/public/igem/BBa_M1010_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2116256
display id: annotation2116256
version: 1
top level: BBa_M1010
location: https://synbiohub.org/public/igem/BBa_M1010/annotation2116256/range2116256/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1010/annotation2116256/range2116256/1
type: Range
persistent identity: range2116256
display id: range2116256
version: 1
top level: BBa_M1010
direction: direction/forward
start: 1
end: 3
orientation: inline

Phasin
type: SequenceAnnotation
persistent identity: annotation2116257
display id: annotation2116257
version: 1
top level: BBa_M1010
location: https://synbiohub.org/public/igem/BBa_M1010/annotation2116257/range2116257/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1010/annotation2116257/range2116257/1
type: Range
persistent identity: range2116257
display id: range2116257
version: 1
top level: BBa_M1010
direction: direction/forward
start: 1
end: 576
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2116258
display id: annotation2116258
version: 1
top level: BBa_M1010
location: https://synbiohub.org/public/igem/BBa_M1010/annotation2116258/range2116258/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1010/annotation2116258/range2116258/1
type: Range
persistent identity: range2116258
display id: range2116258
version: 1
top level: BBa_M1010
direction: direction/forward
start: 583
end: 762
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2116259
display id: annotation2116259
version: 1
top level: BBa_M1010
location: https://synbiohub.org/public/igem/BBa_M1010/annotation2116259/range2116259/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1010/annotation2116259/range2116259/1
type: Range
persistent identity: range2116259
display id: range2116259
version: 1
top level: BBa_M1010
direction: direction/forward
start: 763
end: 768
orientation: inline

BBa_M1027
type: ComponentDefinition
persistent identity: BBa M1027
display id: BBa_M1027
version: 1
was generated by: iGEM to SBOL conversion
description: RBS HlyB RBS HlyD RBS TolC Term
created: 2011-04-19T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: See Subparts
owned by: james
owned by: myers
mutable description: Codes for the HlyB and HlyD membrane proteins necessary for Type I secretion.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B1006
component: BBa_M1028
component: BBa_B0035
component: BBa_M1004
component: BBa_M1003
sequence annotation: BBa_B0035
sequence annotation: BBa_M1003
sequence annotation: BBa_M1004
sequence annotation: BBa_M1028
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_M1027_sequence/1

BBa_B1006
type: Component
persistent identity: component2117590
display id: component2117590
version: 1
top level: BBa_M1027
definition: BBa_B1006
access: public

BBa_M1028
type: Component
persistent identity: component2117585
display id: component2117585
version: 1
top level: BBa_M1027
definition: BBa_M1028
access: public

BBa_B0035
type: Component
persistent identity: component2117581
display id: component2117581
version: 1
top level: BBa_M1027
definition: BBa_B0035
access: public

BBa_M1004
type: Component
persistent identity: component2117580
display id: component2117580
version: 1
top level: BBa_M1027
definition: BBa_M1004
access: public

BBa_B0035
type: Component
persistent identity: component2117576
display id: component2117576
version: 1
top level: BBa_M1027
definition: BBa_B0035
access: public

BBa_M1003
type: Component
persistent identity: component2117575
display id: component2117575
version: 1
top level: BBa_M1027
definition: BBa_M1003
access: public

BBa_B0035
type: Component
persistent identity: component2117571
display id: component2117571
version: 1
top level: BBa_M1027
definition: BBa_B0035
access: public

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117571
display id: annotation2117571
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117571/range2117571/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1027/annotation2117571/range2117571/1
type: Range
persistent identity: range2117571
display id: range2117571
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2117575
display id: annotation2117575
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117575/range2117575/1
component: BBa_M1003

https://synbiohub.org/public/igem/BBa_M1027/annotation2117575/range2117575/1
type: Range
persistent identity: range2117575
display id: range2117575
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 21
end: 2144
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117576
display id: annotation2117576
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117576/range2117576/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1027/annotation2117576/range2117576/1
type: Range
persistent identity: range2117576
display id: range2117576
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 2153
end: 2166
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2117580
display id: annotation2117580
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117580/range2117580/1
component: BBa_M1004

https://synbiohub.org/public/igem/BBa_M1027/annotation2117580/range2117580/1
type: Range
persistent identity: range2117580
display id: range2117580
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 2173
end: 3609
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117581
display id: annotation2117581
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117581/range2117581/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1027/annotation2117581/range2117581/1
type: Range
persistent identity: range2117581
display id: range2117581
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 3618
end: 3631
orientation: inline

BBa_M1028
type: SequenceAnnotation
persistent identity: annotation2117585
display id: annotation2117585
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117585/range2117585/1
component: BBa_M1028

https://synbiohub.org/public/igem/BBa_M1027/annotation2117585/range2117585/1
type: Range
persistent identity: range2117585
display id: range2117585
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 3638
end: 5155
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2117590
display id: annotation2117590
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117590/range2117590/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_M1027/annotation2117590/range2117590/1
type: Range
persistent identity: range2117590
display id: range2117590
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 5164
end: 5202
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_M1003
type: ComponentDefinition
persistent identity: BBa M1003
display id: BBa_M1003
version: 1
was generated by: iGEM to SBOL conversion
description: HlyB membrane protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UT189
owned by: myers
owned by: james
mutable description: Membrane protein involved in type I secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115949
display id: annotation2115949
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
type: Range
persistent identity: range2115949
display id: range2115949
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2115951
display id: annotation2115951
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
type: Range
persistent identity: range2115951
display id: range2115951
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 2121
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115950
display id: annotation2115950
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
type: Range
persistent identity: range2115950
display id: range2115950
version: 1
top level: BBa_M1003
direction: direction/forward
start: 2122
end: 2124
orientation: inline

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: iGEM to SBOL conversion
description: HlyD Membrane Protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyD
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 1434
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1435
end: 1437
orientation: inline

BBa_M1028
type: ComponentDefinition
persistent identity: BBa M1028
display id: BBa_M1028
version: 1
was generated by: iGEM to SBOL conversion
description: tolC-Outer membrane protein
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: E. coli genome, UTI89
owned by: james
owned by: myers
mutable description: TolC is an outer membrane protein that is essential in the type I secretion pathway together with membrane proteins HlyB and HlyD.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Sequence was modified to remove incompatible PstI sites as well as to avoid the presence of any of the Zn finger binding sequences for the Zif268-HIV, Zif23-GCN4, and Blues Zn fingers.
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: TolC
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_M1028_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2117426
display id: annotation2117426
version: 1
top level: BBa_M1028
location: https://synbiohub.org/public/igem/BBa_M1028/annotation2117426/range2117426/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1028/annotation2117426/range2117426/1
type: Range
persistent identity: range2117426
display id: range2117426
version: 1
top level: BBa_M1028
direction: direction/forward
start: 1
end: 3
orientation: inline

TolC
type: SequenceAnnotation
persistent identity: annotation2117428
display id: annotation2117428
version: 1
top level: BBa_M1028
location: https://synbiohub.org/public/igem/BBa_M1028/annotation2117428/range2117428/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1028/annotation2117428/range2117428/1
type: Range
persistent identity: range2117428
display id: range2117428
version: 1
top level: BBa_M1028
direction: direction/forward
start: 1
end: 1515
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2117427
display id: annotation2117427
version: 1
top level: BBa_M1028
location: https://synbiohub.org/public/igem/BBa_M1028/annotation2117427/range2117427/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1028/annotation2117427/range2117427/1
type: Range
persistent identity: range2117427
display id: range2117427
version: 1
top level: BBa_M1028
direction: direction/forward
start: 1516
end: 1518
orientation: inline

https://synbiohub.org/public/igem/BBa_M1014_sequence/1
type: Sequence
persistent identity: BBa M1014 sequence
display id: BBa_M1014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagattagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1026_sequence/1
type: Sequence
persistent identity: BBa M1026 sequence
display id: BBa_M1026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttttttgatgctgcatttgcgtggtttgtttggatgttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1013_sequence/1
type: Sequence
persistent identity: BBa M1013 sequence
display id: BBa_M1013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagattagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1005_sequence/1
type: Sequence
persistent identity: BBa M1005 sequence
display id: BBa_M1005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1010_sequence/1
type: Sequence
persistent identity: BBa M1010 sequence
display id: BBa_M1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaaccttcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgccactagattagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1027_sequence/1
type: Sequence
persistent identity: BBa M1027 sequence
display id: BBa_M1027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1003_sequence/1
type: Sequence
persistent identity: BBa M1003 sequence
display id: BBa_M1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1004_sequence/1
type: Sequence
persistent identity: BBa M1004 sequence
display id: BBa_M1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1028_sequence/1
type: Sequence
persistent identity: BBa M1028 sequence
display id: BBa_M1028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z