Complete construct for phasin secretion together with zinc finger binding sites
This construct is designed to exist in a plasmid separate from a plasmid coding for the zinc finger-fused enzymes involved in the PHB enzymatic pathway. This construct codes for phasin targeted for secretion by the Type I pathway as well as the Type I associated membrane proteins HlyB and HlyD. It also contains a repeated sequence that allows ordered binding of Zif268-HIV, Zif23-GCN 4, and "Blues" Zinc fingers.
Scaffold contain the DNA sequences where the zinc fingers HIVC GN23 and blue zinc finger bind.
Complete Construct for Type I Phasin Secretion
This construct codes for phasin targeted for secretion with the HlyA signal peptide. It also codes for the HlyB and HlyD membrane proteins necessary for type I secretion. Coding regions are controlled under high levels of constitutive expression.
Constitutive promoter with high transcriptional levels reported. RBS has high level of translation.
This is a modification of the part BBa_K208029 which already exists in the registry. This part contains the scar that corresponds to Silver Fusion.
Codes for the HlyB and HlyD membrane proteins necessary for Type I secretion.
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
Membrane protein involved in type I secretion.
Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
TolC is an outer membrane protein that is essential in the type I secretion pathway together with membrane proteins HlyB and HlyD.
Sequence was modified to remove incompatible PstI sites as well as to avoid the presence of any of the Zn finger binding sequences for the Zif268-HIV, Zif23-GCN4, and Blues Zn fingers.
Conversion of the iGEM parts registry to SBOL2.1