LOCUS       BBa_M1017               3173 bp    DNA     linear       01-Jun-2024
DEFINITION  Silver Fusion Compatible PhaB gene with zif23-GCN4
ACCESSION   BBa_M1017
VERSION     BBa_M1017.1  
FEATURES             Location/Qualifiers
     CDS             1013..1099
                     /label=Finger 2
     CDS             1100..1159
                     /label=Finger 3
     misc_feature    1160..1174
                     /label=linker
     CDS             1175..1270
                     /label=GCN4: leucine zipper
     misc_feature    1271..1303
                     /label=LVA
     misc_feature    1304..1309
                     /label=2
     misc_feature    1052..1072
                     /label=Recognition site
     misc_feature    1136..1156
                     /label=Recognition site
     misc_feature    1310..1334
                     /label=Help:Barcodes
     CDS             1010..1309
                     /label=BBa_C2001
     misc_feature    1341..1343
                     /label=start
     misc_feature    2061..2063
                     /label=original stop codon
     misc_feature    2064..2066
                     /label=inserted second stop
     CDS             1341..2066
                     /label=BBa_K125502
     RBS             2075..2088
                     /label=BBa_B0035
     CDS             2098..2184
                     /label=Finger 2
     CDS             2185..2244
                     /label=Finger 3
     misc_feature    2245..2259
                     /label=linker
     CDS             2260..2355
                     /label=GCN4: leucine zipper
     misc_feature    2356..2388
                     /label=LVA
     misc_feature    2389..2394
                     /label=2
     misc_feature    2137..2157
                     /label=Recognition site
     misc_feature    2221..2241
                     /label=Recognition site
     misc_feature    2395..2419
                     /label=Help:Barcodes
     CDS             2095..2394
                     /label=BBa_C2001
     misc_feature    2426..2428
                     /label=start
     misc_feature    3146..3148
                     /label=original stop codon
     misc_feature    3149..3151
                     /label=inserted second stop
     CDS             2426..3151
                     /label=BBa_K125502
     RBS             3160..3173
                     /label=BBa_B0035
ORIGIN
        1 attaaagagg agaaatgaag cccttccagt gtcgaatctg catgcgtaac ttcagtcgta
       61 gtgaccacct taccacccac atccgcaccc acacaggcga gaagcctttt gcctgtgaca
      121 tttgtgggag gaagtttgcc aggagtgatg aacgcaagag gcatagaaag ttgcaacata
      181 tgaaacaact tgaagacaag gttgaagaat tgctttcgaa aaattatcac ttggaaaatg
      241 aggttgccag attaaagaaa ttagttggcg aacgctaata aactagaatg ttaagtcttg
      301 gtttggaaga taaagtaatc gtggtcaccg gcggcaatcg gggcatcggc gcggcgatcg
      361 tgaaattact ccaggaaatg ggggccaaag tagcttttac cgatttagct acggacgggg
      421 gtaatactga agccctgggg gtggtggcca acgtcaccga tttggaatcc atgacggcgg
      481 cggcagcgga aatcaccgat aagctgggcc ccgtttacgg tgtggtggcc aatgccggta
      541 tcaccaaaga caactttttc ccaaaattaa cccccgccga ttgggatgca gtgttgaacg
      601 ttaacttgaa aggggtagcc tacagcatta agcctttcat cgaaggcatg tatgaacgga
      661 aagccggctc cattgtggcc attagttcca tctccgggga gcggggtaac gtcggtcaaa
      721 ctattccgcc actaaagctg gggtaattgg catgatgaaa tccctggctc gggaaggggc
      781 ccggtatgga gtgcgggcca atgcagtagc ccctggtttc attgacaccg aaatgacttt
      841 ggcgatccgg gaagatattc gggaaaaaat taccaaggaa atccccttcc gccgttttgg
      901 taaaccggag gaaattgcct gggcggtggc ctttttactt tcccccgtag ccagtagcta
      961 tgtcaccggc gaagtattac gggtaaatgg ggcccaccac acctactaga tgaaaccgtt
     1021 ccagtgccgt atctgcatgc gtaacttctc ccgttccgac cacctgacca cccacatccg
     1081 tacccacacc ggtgaaaaac cgttcgcttg cgacatctgc ggtcgtaaat tcgctcgttc
     1141 cgacgaacgt aaacgtcacc gtaaattgca gcacatgaaa cagctggaag acaaagttga
     1201 agaactgctg tccaaaaact accacctgga aaacgaagtt gctcgtctga aaaaactggt
     1261 tggtgaacgt gctgctaacg acgaaaacta cgctctggtt gcttaataac tctgatagtg
     1321 ctagtgtaga tctctactag atgttaagtc ttggtttgga agataaagta atcgtggtca
     1381 ccggcggcaa tcggggcatc ggcgcggcga tcgtgaaatt actccaggaa atgggggcca
     1441 aagtagcttt taccgattta gctacggacg ggggtaatac tgaagccctg ggggtggtgg
     1501 ccaacgtcac cgatttggaa tccatgacgg cggcggcagc ggaaatcacc gataagctgg
     1561 gccccgttta cggtgtggtg gccaatgccg gtatcaccaa agacaacttt ttcccaaaat
     1621 taacccccgc cgattgggat gcagtgttga acgttaactt gaaaggggta gcctacagca
     1681 ttaagccttt catcgaaggc atgtatgaac ggaaagccgg ctccattgtg gccattagtt
     1741 ccatctccgg ggagcggggt aacgtcggtc aaactaacta ttccgccact aaagctgggg
     1801 taattggcat gatgaaatcc ctggctcggg aaggggcccg gtatggagtg cgggccaatg
     1861 cagtagcccc tggtttcatt gacaccgaaa tgactttggc gatccgggaa gatattcggg
     1921 aaaaaattac caaggaaatc cccttccgcc gttttggtaa accggaggaa attgcctggg
     1981 cggtggcctt tttactttcc cccgtagcca gtagctatgt caccggcgaa gtattacggg
     2041 taaatggggc ccaccacacc taataatact agagattaaa gaggagaata ctagatgaaa
     2101 ccgttccagt gccgtatctg catgcgtaac ttctcccgtt ccgaccacct gaccacccac
     2161 atccgtaccc acaccggtga aaaaccgttc gcttgcgaca tctgcggtcg taaattcgct
     2221 cgttccgacg aacgtaaacg tcaccgtaaa ttgcagcaca tgaaacagct ggaagacaaa
     2281 gttgaagaac tgctgtccaa aaactaccac ctggaaaacg aagttgctcg tctgaaaaaa
     2341 ctggttggtg aacgtgctgc taacgacgaa aactacgctc tggttgctta ataactctga
     2401 tagtgctagt gtagatctct actagatgtt aagtcttggt ttggaagata aagtaatcgt
     2461 ggtcaccggc ggcaatcggg gcatcggcgc ggcgatcgtg aaattactcc aggaaatggg
     2521 ggccaaagta gcttttaccg atttagctac ggacgggggt aatactgaag ccctgggggt
     2581 ggtggccaac gtcaccgatt tggaatccat gacggcggcg gcagcggaaa tcaccgataa
     2641 gctgggcccc gtttacggtg tggtggccaa tgccggtatc accaaagaca actttttccc
     2701 aaaattaacc cccgccgatt gggatgcagt gttgaacgtt aacttgaaag gggtagccta
     2761 cagcattaag cctttcatcg aaggcatgta tgaacggaaa gccggctcca ttgtggccat
     2821 tagttccatc tccggggagc ggggtaacgt cggtcaaact aactattccg ccactaaagc
     2881 tggggtaatt ggcatgatga aatccctggc tcgggaaggg gcccggtatg gagtgcgggc
     2941 caatgcagta gcccctggtt tcattgacac cgaaatgact ttggcgatcc gggaagatat
     3001 tcgggaaaaa attaccaagg aaatcccctt ccgccgtttt ggtaaaccgg aggaaattgc
     3061 ctgggcggtg gcctttttac tttcccccgt agccagtagc tatgtcaccg gcgaagtatt
     3121 acgggtaaat ggggcccacc acacctaata atactagaga ttaaagagga gaa
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