BBa_M1017 1 BBa_M1017 Silver Fusion Compatible PhaB gene with zif23-GCN4 2011-04-12T11:00:00Z 2015-05-08T01:13:51Z N/A This sequence includes the RBS (with the best compatibility for the use of zinc fingers), PhaB enzyme, zif23-GCN4, and a silver fusion scar tissue sequence. The silver fusion scar tissue ensures the binding within a silver fusion vector. The zinc finger is there to help with the binding and translation of the PhaB enzyme. PhaB is a biodegradable plastic enzyme for polyhydroxylakanoate (PHB). true false _768_ 0 8965 9 Discontinued false This sequence is silver fusion compatible and has a zinc finger included. false Michaela Hugie component2115948 1 BBa_B0035 component2115942 1 BBa_C2001 component2115947 1 BBa_K125502 component2115933 1 BBa_B0035 component2115930 1 BBa_K125502 component2115919 1 BBa_C2001 annotation2115919 1 BBa_C2001 range2115919 1 1010 1309 annotation2115948 1 BBa_B0035 range2115948 1 3160 3173 annotation2115947 1 BBa_K125502 range2115947 1 2426 3151 annotation2115942 1 BBa_C2001 range2115942 1 2095 2394 annotation2115933 1 BBa_B0035 range2115933 1 2075 2088 annotation2115930 1 BBa_K125502 range2115930 1 1341 2066 BBa_C2001 1 zif23 Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli) 2004-01-29T12:00:00Z 2015-08-31T04:07:25Z Protein Engineering Gods from the Pabo Lab Released HQ 2013 Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added. false false _1_ 0 24 7 In stock false This protein should form a dimer and bind a specific operator site, thus repressing transcription. false Drew Endy annotation2214011 1 Help:Barcodes range2214011 1 301 325 annotation318109 1 Recognition site range318109 1 127 147 annotation318104 1 linker range318104 1 151 165 annotation318101 1 Finger 2 range318101 1 4 90 annotation318103 1 Finger 3 range318103 1 91 150 annotation318107 1 2 range318107 1 295 300 annotation318105 1 GCN4: leucine zipper range318105 1 166 261 annotation318108 1 Recognition site range318108 1 43 63 annotation318106 1 LVA range318106 1 262 294 BBa_B0035 1 BBa_B0035 RBS (B0030 derivative) 2004-01-27T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 Not in stock false false Jason Kelly BBa_K125502 1 BBa_K125502 phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993) 2008-08-09T11:00:00Z 2015-05-08T01:09:45Z Synechocystis sp. PCC6803 phaB false false _179_ 0 2680 9 It's complicated false PHB biosynthesis pathway false Norman Wang annotation1971709 1 original stop codon range1971709 1 721 723 annotation1971708 1 start range1971708 1 1 3 annotation1971710 1 inserted second stop range1971710 1 724 726 BBa_K125502_sequence 1 atgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataa BBa_B0035_sequence 1 attaaagaggagaa BBa_M1017_sequence 1 attaaagaggagaaatgaagcccttccagtgtcgaatctgcatgcgtaacttcagtcgtagtgaccaccttaccacccacatccgcacccacacaggcgagaagccttttgcctgtgacatttgtgggaggaagtttgccaggagtgatgaacgcaagaggcatagaaagttgcaacatatgaaacaacttgaagacaaggttgaagaattgctttcgaaaaattatcacttggaaaatgaggttgccagattaaagaaattagttggcgaacgctaataaactagaatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataatactagagattaaagaggagaatactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataatactagagattaaagaggagaa BBa_C2001_sequence 1 atgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z