LOCUS       BBa_M10210              2509 bp    DNA     linear       22-Nov-2024
DEFINITION  {Pbad.rbs.prepro.StrepTag}{AG4}{CPG_L6!}{dblTerm}
ACCESSION   BBa_M10210
VERSION     BBa_M10210.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     CDS             1461..1496
                     /label=BBa_M10023
     misc_feature    1676..1693
                     /label=Linker
     CDS             1694..2366
                     /label=Native N terminus
     CDS             1505..1675
                     /label=Native C terminus
     CDS             1505..2366
                     /label=BBa_M10011
     terminator      2375..2509
                     /label=BBa_K112903
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagag aacccgtctt ctctgttccg ttacctgccg tctgactact
     1501 agaggatgat attgaacgtg aaggccatga ttttaaccgt gtaggtttat tttacggtta
     1561 tgatttccag aacggacttt ccgtttcgct ggaatacgcg tttgagtggc aggatcacga
     1621 cgaaggcgac agtgataaat tccattatgc aggtgtcggc gtaaattact cgttcggatc
     1681 taagtctcgt cgcgaggaaa ggaacgactg gcactttaat atcggcgcga tgtacgaaat
     1741 agaaaacgtc gagggttatg gcgaagatat ggatgggctg gcggagcctt cagtctattt
     1801 taatgccgcc aacgggccgt ggagaattgc tctggcctat tatcaggaag ggccggtaga
     1861 ttatagcgcg ggtaaacgtg gaacgtggtt tgatcgcccg gagctggagg tgcattatca
     1921 gttcctcgaa aacgatgatt tcagtttcgg cctgaccggc ggtttccgta attatggtta
     1981 tcactacgtt gatgaaccgg gtaaagacac ggcgaatatg cagcgctgga aaatcgcgcc
     2041 agactgggat gtgaaactga ctgacgattt acgtttcaac ggttggttgt cgatgtataa
     2101 atttgccaac gatctgaaca ctaccggtta cgctgatacc cgtgtcgaaa cggaaacagg
     2161 tctgcaatat accttcaacg aaacggttgc cttgcgagtg aactattatc ttgagcgcgg
     2221 cttcaatatg gacgacagcc gcaataacgg tgagttttcc acgcaagaaa ttcgcgccta
     2281 tttgccgctg acgctcggca accactcggt gacgccgtat acgcgcattg ggctggatcg
     2341 ctggagtaac tgggactggc agtaagtact agaggatctc caggcatcaa ataaaacgaa
     2401 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2461 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatag
//