BBa_M10093
1
BBa_M10093
{Pbad.rbs.prepro.StrepTag˃}
2009-04-26T11:00:00Z
2015-05-08T01:13:51Z
PCR from synthesized DNAs
Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar.
false
false
_271_
0
483
271
Not in stock
false
N/A
false
John Anderson
annotation2002590
1
Pbad
range2002590
1
956
1193
annotation2002591
1
PrePro Sequence
range2002591
1
1290
1358
annotation2002589
1
AraC
range2002589
1
50
805
annotation2002592
1
Streptavidin Binding Peptide
range2002592
1
1374
1412
annotation2002593
1
rbs
range2002593
1
1274
1289
BBa_K112903
1
BBa_K112903
b0015 terminator
2008-10-23T11:00:00Z
2015-05-08T01:09:19Z
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10023
1
AG4
{˂AG4˃} Silver binding peptide
2009-05-06T11:00:00Z
2015-05-08T01:13:51Z
Wobble Odvs002F/Odvs002R to make M10023 (Bdvs002) (67 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10023 {<AG4>} (3222bp)
----
Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC
Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG
later
false
false
_271_
0
2047
271
Not in stock
true
later
false
Dirk van Swaay
BBa_M10212
1
BBa_M10212
{Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂upaG_short!}{dblTerm}
2009-05-07T11:00:00Z
2015-05-08T01:13:52Z
BglBricks standard assembly
{{BioE140LSpr09-AssayOverview}}
{{BioE140LSpr09-Streptavidin}}
{{BioE140LSpr09-Growth}}
{{BioE140LSpr09-Silver}}
{{BioE140LSpr09-Autoaggregation}}
false
false
_271_
0
4740
271
Not in stock
true
false
Simina Ticau
component2002643
1
BBa_M10023
component2002642
1
BBa_K112999
component2002645
1
BBa_M10026
component2002647
1
BBa_K112903
component2002641
1
BBa_M10093
component2002644
1
BBa_K112999
component2002646
1
BBa_K112999
annotation2002647
1
BBa_K112903
range2002647
1
1822
1956
annotation2002646
1
BBa_K112999
range2002646
1
1808
1813
annotation2002642
1
BBa_K112999
range2002642
1
1461
1466
annotation2002644
1
BBa_K112999
range2002644
1
1519
1524
annotation2002645
1
BBa_M10026
range2002645
1
1533
1799
annotation2002641
1
BBa_M10093
range2002641
1
1
1452
annotation2002643
1
BBa_M10023
range2002643
1
1475
1510
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10026
1
BBa_M10026
{˂upaG_short!} upaG autotransporter short version
2009-04-26T11:00:00Z
2015-05-08T01:13:51Z
CFT073 genomic DNA
translocation to cell surface
false
false
_271_
0
4740
271
Not in stock
true
none
false
Simina Ticau
BBa_M10212_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagagggatcttactagagaacccgtcttctctgttccgttacctgccgtctgactactagagggatcttactagaggttgagatggataacaaactgtccaaaactgaaagcaagctgagtggtggtatcgcttctgcaatggcaatgaccggtctgccgcaggcttacacgccgggtgccagcatggcctctattggtggcggtacttacaacggtgaatcggctgttgctttaggtgtgtcgatggtgagcgccaatggtcgttgggtctacaaattacaaggtagtaccaatagccagggtgaatactccgccgcactcggtgccggtattcagtggtaatactagagggatcttactagaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_K112903_sequence
1
gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_M10026_sequence
1
gttgagatggataacaaactgtccaaaactgaaagcaagctgagtggtggtatcgcttctgcaatggcaatgaccggtctgccgcaggcttacacgccgggtgccagcatggcctctattggtggcggtacttacaacggtgaatcggctgttgctttaggtgtgtcgatggtgagcgccaatggtcgttgggtctacaaattacaaggtagtaccaatagccagggtgaatactccgccgcactcggtgccggtattcagtggtaa
BBa_M10023_sequence
1
aacccgtcttctctgttccgttacctgccgtctgac
BBa_K112999_sequence
1
ggatct
BBa_M10093_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z