BBa_K112903
1
BBa_K112903
b0015 terminator
2008-10-23T11:00:00Z
2015-05-08T01:09:19Z
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10023
1
AG4
{˂AG4˃} Silver binding peptide
2009-05-06T11:00:00Z
2015-05-08T01:13:51Z
Wobble Odvs002F/Odvs002R to make M10023 (Bdvs002) (67 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10023 {<AG4>} (3222bp)
----
Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC
Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG
later
false
false
_271_
0
2047
271
Not in stock
true
later
false
Dirk van Swaay
BBa_M10213
1
BBa_M10213
{Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂Ag43_short!}{dblTerm}
2009-05-09T11:00:00Z
2015-05-08T01:13:52Z
BglBricks standard assembly
{{BioE140LSpr09-AssayOverview}}
{{BioE140LSpr09-Growth}}
{{BioE140LSpr09-Silver}}
false
false
_271_
0
4738
271
Not in stock
true
waiting
false
Sadao Ota
component2002671
1
BBa_M10023
component2002674
1
BBa_K112999
component2002669
1
BBa_M10093
component2002670
1
BBa_K112999
component2002673
1
BBa_M10034
component2002672
1
BBa_K112999
component2002675
1
BBa_K112903
annotation2002674
1
BBa_K112999
range2002674
1
1670
1675
annotation2002670
1
BBa_K112999
range2002670
1
1461
1466
annotation2002675
1
BBa_K112903
range2002675
1
1684
1818
annotation2002671
1
BBa_M10023
range2002671
1
1475
1510
annotation2002673
1
BBa_M10034
range2002673
1
1533
1661
annotation2002669
1
BBa_M10093
range2002669
1
1
1452
annotation2002672
1
BBa_K112999
range2002672
1
1519
1524
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10034
1
BBa_M10034
{˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter
2009-05-09T11:00:00Z
2015-05-08T01:13:51Z
The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome.
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at
This part is an actual transporter portion of an antigen 43 autotransporter display system based on this reference: PMID: 12107137.
false
false
_271_
0
4738
271
Not in stock
true
Bglbricking of M10034, Ag43 (autotransporter only)
PCR Oso001 and Oso002 on E. coli MG1655 genomic DNA (937 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495KC-M10034 {<Ag43_short!}
----------------------------
Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg
Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg
false
Sadao Ota
BBa_M10093
1
BBa_M10093
{Pbad.rbs.prepro.StrepTag˃}
2009-04-26T11:00:00Z
2015-05-08T01:13:51Z
PCR from synthesized DNAs
Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar.
false
false
_271_
0
483
271
Not in stock
false
N/A
false
John Anderson
annotation2002592
1
Streptavidin Binding Peptide
range2002592
1
1374
1412
annotation2002593
1
rbs
range2002593
1
1274
1289
annotation2002589
1
AraC
range2002589
1
50
805
annotation2002590
1
Pbad
range2002590
1
956
1193
annotation2002591
1
PrePro Sequence
range2002591
1
1290
1358
BBa_K112903_sequence
1
gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_M10213_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagagggatcttactagagaacccgtcttctctgttccgttacctgccgtctgactactagagggatcttactagagggttctggtccgacccagtctcactacggtcagtgcggtggtatcggttactctggtccgaccgtttgcgcttctggtaccacctgccaggttctgaacccgtactactctcagtgcctgggttctggttactagagggatcttactagaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_M10023_sequence
1
aacccgtcttctctgttccgttacctgccgtctgac
BBa_K112999_sequence
1
ggatct
BBa_M10034_sequence
1
ggttctggtccgacccagtctcactacggtcagtgcggtggtatcggttactctggtccgaccgtttgcgcttctggtaccacctgccaggttctgaacccgtactactctcagtgcctgggttctggt
BBa_M10093_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z