LOCUS BBa_M10214 3039 bp DNA linear 05-Nov-2024 DEFINITION {Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂espP(beta)!}{dblT erm} ACCESSION BBa_M10214 VERSION BBa_M10214.1 FEATURES Location/Qualifiers CDS 50..805 /label=AraC promoter 956..1193 /label=Pbad CDS 1290..1358 /label=PrePro Sequence CDS 1374..1412 /label=Streptavidin Binding Peptide RBS 1274..1289 /label=rbs misc_feature 1..1452 /label=BBa_M10093 misc_feature 1461..1466 /label=BBa_K112999 CDS 1475..1510 /label=BBa_M10023 misc_feature 1519..1524 /label=BBa_K112999 CDS 1533..2882 /label=BBa_M10044 misc_feature 2891..2896 /label=BBa_K112999 terminator 2905..3039 /label=BBa_K112903 ORIGIN 1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac 301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc 481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga 1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc 1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca 1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac 1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg 1441 cagtctgggc agtactagag ggatcttact agagaacccg tcttctctgt tccgttacct 1501 gccgtctgac tactagaggg atcttactag aggccccgtc agcatctgcc actatgaaca 1561 acacctggtg gcaactaacc ggagattctg cgctgaaaac actgaaaagt acaaacagca 1621 tggtctattt cactgacagc gcaaacaata agaaattcca tacgctgacg gtcgatgagc 1681 tggcaaccag caacagcgcc tatgcgatgc gtacaaacct ttctgaatca gacaaactgg 1741 aggtcaaaaa acacttgtct ggtgagaaca atattttact cgttgatttc cttcagaaac 1801 caacgcctga aaaacaactg aatattgaac tggtaagcgc gccaaaagac accaatgaaa 1861 atgtctttaa agccagtaaa caaaccattg gtttcagtga tgtaacgccg gtcattacaa 1921 ccagggaaac cgatgacaaa ataacatggt cactgacagg ctataacacg gtagcaaaca 1981 aggaagcaac ccggaatgcc gccgccctgt tctctgttga ctataaagcg tttctgaacg 2041 aggtcaacaa cctgaacaaa cgtatgggtg acctgcgtga tatcaacggc gaagccggtg 2101 catgggcacg catcatgagc ggtaccggct ctgccagtgg tggtttcagt gacaactaca 2161 cgcacgttca ggtcggggtc gacaaaaaac acgagctgga cggactggat ttgtttaccg 2221 gtttcactgt cacacacact gacagcagtg cctccgccga tgttttcagt ggtaaaacga 2281 agtctgtggg ggctggcctg tatgcttccg ccatgtttga ttccggtgcc tatatcgacc 2341 tgattggcaa gtatgttcac catgataatg agtacactgc aacctttgcc ggactcggaa 2401 cccgtgatta cagcacgcat tcatggtatg ccggtgcaga agcgggctac cgctatcatg 2461 tcactgagga tgcctggatt gagccacagg ctgagctggt ttacggttct gtatccggta 2521 aacagtttgc atggaaggac cagggaatgc atctgtccat gaaggacaag gactacaatc 2581 cgctgattgg ccgaacgggt gtggatgtgg gtaaatcctt ctctggtaag gactggaaag 2641 tgacagcccg tgccggtctg ggctaccagt tcgacctgct ggctaacggc gaaaccgtat 2701 tgcgggatgc atctggtgaa aaacgcatca aaggtgaaaa ggacagccgt atgctgatgt 2761 ccgttggcct gaatgcagaa atcagggata acgtccgctt tggactggag tttgagaaat 2821 ccgcctttgg taagtacaac gttgataatg ctgtcaacgc taatttccgt tactcgttct 2881 gatactagag ggatcttact agaggatctc caggcatcaa ataaaacgaa aggctcagtc 2941 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3001 cactggctca ccttcgggtg ggcctttctg cgtttatag //