BBa_M10214
1
BBa_M10214
{Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂espP(beta)!}{dblTerm}
2009-05-09T11:00:00Z
2015-05-08T01:13:52Z
BglBricks Assembly
AG4 passenger part attached to a espP(beta) displayer
false
false
_271_
0
4744
271
Not in stock
false
N/A
false
Sam Ng
component2002725
1
BBa_M10044
component2002722
1
BBa_K112999
component2002726
1
BBa_K112999
component2002727
1
BBa_K112903
component2002721
1
BBa_M10093
component2002724
1
BBa_K112999
component2002723
1
BBa_M10023
annotation2002726
1
BBa_K112999
range2002726
1
2891
2896
annotation2002721
1
BBa_M10093
range2002721
1
1
1452
annotation2002725
1
BBa_M10044
range2002725
1
1533
2882
annotation2002723
1
BBa_M10023
range2002723
1
1475
1510
annotation2002727
1
BBa_K112903
range2002727
1
2905
3039
annotation2002722
1
BBa_K112999
range2002722
1
1461
1466
annotation2002724
1
BBa_K112999
range2002724
1
1519
1524
BBa_K112903
1
BBa_K112903
b0015 terminator
2008-10-23T11:00:00Z
2015-05-08T01:09:19Z
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10044
1
BBa_M10044
{˂espP(beta)!} autotransporter
2009-05-09T11:00:00Z
2015-05-08T01:13:51Z
Escherichia coli O157:H7 str. Sakai
The espP autotransporter is produced by Escherichia coli 0157:H7. This part contains the truncated 'beta domain' that aids in the transportation of the large N-terminal 'passenger domain' across the outer membrane. PMID: 16262782
false
false
_271_
0
4744
271
Not in stock
true
This part is a BglBricks (BBb). No prepro; Begins with amino acid 852 and extends to the stop codon. Part was inserted into pBca9495KC-Bca1144#5.
false
Sam Ng
BBa_M10023
1
AG4
{˂AG4˃} Silver binding peptide
2009-05-06T11:00:00Z
2015-05-08T01:13:51Z
Wobble Odvs002F/Odvs002R to make M10023 (Bdvs002) (67 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10023 {<AG4>} (3222bp)
----
Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC
Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG
later
false
false
_271_
0
2047
271
Not in stock
true
later
false
Dirk van Swaay
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10093
1
BBa_M10093
{Pbad.rbs.prepro.StrepTag˃}
2009-04-26T11:00:00Z
2015-05-08T01:13:51Z
PCR from synthesized DNAs
Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar.
false
false
_271_
0
483
271
Not in stock
false
N/A
false
John Anderson
annotation2002592
1
Streptavidin Binding Peptide
range2002592
1
1374
1412
annotation2002593
1
rbs
range2002593
1
1274
1289
annotation2002589
1
AraC
range2002589
1
50
805
annotation2002590
1
Pbad
range2002590
1
956
1193
annotation2002591
1
PrePro Sequence
range2002591
1
1290
1358
BBa_K112903_sequence
1
gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_M10044_sequence
1
gccccgtcagcatctgccactatgaacaacacctggtggcaactaaccggagattctgcgctgaaaacactgaaaagtacaaacagcatggtctatttcactgacagcgcaaacaataagaaattccatacgctgacggtcgatgagctggcaaccagcaacagcgcctatgcgatgcgtacaaacctttctgaatcagacaaactggaggtcaaaaaacacttgtctggtgagaacaatattttactcgttgatttccttcagaaaccaacgcctgaaaaacaactgaatattgaactggtaagcgcgccaaaagacaccaatgaaaatgtctttaaagccagtaaacaaaccattggtttcagtgatgtaacgccggtcattacaaccagggaaaccgatgacaaaataacatggtcactgacaggctataacacggtagcaaacaaggaagcaacccggaatgccgccgccctgttctctgttgactataaagcgtttctgaacgaggtcaacaacctgaacaaacgtatgggtgacctgcgtgatatcaacggcgaagccggtgcatgggcacgcatcatgagcggtaccggctctgccagtggtggtttcagtgacaactacacgcacgttcaggtcggggtcgacaaaaaacacgagctggacggactggatttgtttaccggtttcactgtcacacacactgacagcagtgcctccgccgatgttttcagtggtaaaacgaagtctgtgggggctggcctgtatgcttccgccatgtttgattccggtgcctatatcgacctgattggcaagtatgttcaccatgataatgagtacactgcaacctttgccggactcggaacccgtgattacagcacgcattcatggtatgccggtgcagaagcgggctaccgctatcatgtcactgaggatgcctggattgagccacaggctgagctggtttacggttctgtatccggtaaacagtttgcatggaaggaccagggaatgcatctgtccatgaaggacaaggactacaatccgctgattggccgaacgggtgtggatgtgggtaaatccttctctggtaaggactggaaagtgacagcccgtgccggtctgggctaccagttcgacctgctggctaacggcgaaaccgtattgcgggatgcatctggtgaaaaacgcatcaaaggtgaaaaggacagccgtatgctgatgtccgttggcctgaatgcagaaatcagggataacgtccgctttggactggagtttgagaaatccgcctttggtaagtacaacgttgataatgctgtcaacgctaatttccgttactcgttctga
BBa_M10023_sequence
1
aacccgtcttctctgttccgttacctgccgtctgac
BBa_M10214_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagagggatcttactagagaacccgtcttctctgttccgttacctgccgtctgactactagagggatcttactagaggccccgtcagcatctgccactatgaacaacacctggtggcaactaaccggagattctgcgctgaaaacactgaaaagtacaaacagcatggtctatttcactgacagcgcaaacaataagaaattccatacgctgacggtcgatgagctggcaaccagcaacagcgcctatgcgatgcgtacaaacctttctgaatcagacaaactggaggtcaaaaaacacttgtctggtgagaacaatattttactcgttgatttccttcagaaaccaacgcctgaaaaacaactgaatattgaactggtaagcgcgccaaaagacaccaatgaaaatgtctttaaagccagtaaacaaaccattggtttcagtgatgtaacgccggtcattacaaccagggaaaccgatgacaaaataacatggtcactgacaggctataacacggtagcaaacaaggaagcaacccggaatgccgccgccctgttctctgttgactataaagcgtttctgaacgaggtcaacaacctgaacaaacgtatgggtgacctgcgtgatatcaacggcgaagccggtgcatgggcacgcatcatgagcggtaccggctctgccagtggtggtttcagtgacaactacacgcacgttcaggtcggggtcgacaaaaaacacgagctggacggactggatttgtttaccggtttcactgtcacacacactgacagcagtgcctccgccgatgttttcagtggtaaaacgaagtctgtgggggctggcctgtatgcttccgccatgtttgattccggtgcctatatcgacctgattggcaagtatgttcaccatgataatgagtacactgcaacctttgccggactcggaacccgtgattacagcacgcattcatggtatgccggtgcagaagcgggctaccgctatcatgtcactgaggatgcctggattgagccacaggctgagctggtttacggttctgtatccggtaaacagtttgcatggaaggaccagggaatgcatctgtccatgaaggacaaggactacaatccgctgattggccgaacgggtgtggatgtgggtaaatccttctctggtaaggactggaaagtgacagcccgtgccggtctgggctaccagttcgacctgctggctaacggcgaaaccgtattgcgggatgcatctggtgaaaaacgcatcaaaggtgaaaaggacagccgtatgctgatgtccgttggcctgaatgcagaaatcagggataacgtccgctttggactggagtttgagaaatccgcctttggtaagtacaacgttgataatgctgtcaacgctaatttccgttactcgttctgatactagagggatcttactagaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_K112999_sequence
1
ggatct
BBa_M10093_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z