LOCUS BBa_M10217 3261 bp DNA linear 05-Nov-2024 DEFINITION {Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂TshA!}{dblTerm} ACCESSION BBa_M10217 VERSION BBa_M10217.1 FEATURES Location/Qualifiers CDS 50..805 /label=AraC promoter 956..1193 /label=Pbad CDS 1290..1358 /label=PrePro Sequence CDS 1374..1412 /label=Streptavidin Binding Peptide RBS 1274..1289 /label=rbs misc_feature 1..1452 /label=BBa_M10093 misc_feature 1461..1466 /label=BBa_K112999 CDS 1475..1510 /label=BBa_M10023 misc_feature 1519..1524 /label=BBa_K112999 CDS 1533..3104 /label=BBa_M10088 misc_feature 3113..3118 /label=BBa_K112999 terminator 3127..3261 /label=BBa_K112903 ORIGIN 1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac 301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc 481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga 1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc 1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca 1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac 1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg 1441 cagtctgggc agtactagag ggatcttact agagaacccg tcttctctgt tccgttacct 1501 gccgtctgac tactagaggg atcttactag agacactttt acctgtatac gattatgccg 1561 gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac agtatgttgt 1621 caggtaatat caatgttcag gataaaggga ctgtcaccct cggaggggaa ggggaactga 1681 gtcctgacct gactcttcag aatcagatgt tgtacagcct gtttaacggg taccgcaata 1741 cctggagcgg gagcctgaat gcaccggatg ccaccgtcag catgacagac acccagtggt 1801 cgatgaacgg aaactccacg gcaggaaata tgaaacttaa ccggacaata gtcggtttta 1861 acgggggaac atcaccgttc acgacactga caacagataa tctggacgcg gttcagtcag 1921 catttgtcat gcgtacagac cttaacaagg cagacaaact ggtgataaac aagtcggcaa 1981 caggtcatga caacagcatc tgggttaact tcctgaaaaa accttctaac aaggacacgc 2041 ttgatattcc actggtcagc gcacctgaag cgacagctga taatctgttc agggcatcaa 2101 cacgggttgt gggattcagt gatgtcaccc ccatccttag tgtcagaaaa gaggacggga 2161 aaaaagagtg ggtcctcgat ggttaccagg ttgcacgtaa cgacggccag ggtaaggctg 2221 ccgccacatt catgcacatc agctataaca acttcatcac tgaagttaac aacctgaaca 2281 aacgcatggg cgatttgagg gatattaacg gcgaagccgg tacgtgggtg cgtctgctga 2341 acggttccgg ctctgctgat ggcggtttca ctgaccacta taccctgctg cagatggggg 2401 ctgaccgtaa gcacgaactg ggaagtatgg acctgtttac cggcgtgatg gccacctaca 2461 ctgacacaga tgcgtcagca gacctgtaca gcggtaaaac aaaatcatgg ggtggtggtt 2521 tctatgccag tggtctgttc cggtccggcg cttactttga tttgattgcc aaatatattc 2581 acaatgaaaa caaatatgac ctgaactttg ccggagctgg taaacagaac ttccgcagcc 2641 attcactgta tgcaggtgca gaagtcggat accgttatca tctgacagat acgacgtttg 2701 ttgaacctca ggcggaactg gtctggggaa gactgcaggg ccaaacattt aactggaacg 2761 acagtggaat ggatgtctca atgcgtcgta acagcgttaa tcctctggta ggcagaaccg 2821 gcgttgtttc cggtaaaacc ttcagtggta aggactggag tctgacagcc cgtgccggcc 2881 tgcattatga gttcgatctg acggacagtg ctgacgttca cctgaaggat gcagcgggag 2941 aacatcagat taatggcaga aaagacggtc gtatgcttta cggtgtgggg ttaaatgccc 3001 ggtttggcga caatacgcgt ctggggctgg aagttgaacg ctctgcattc ggtaaataca 3061 acacagatga tgcgataaac gctaatattc gttattcatt ctgatactag agggatctta 3121 ctagaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3181 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 3241 tgggcctttc tgcgtttata g //