LOCUS       BBa_M10218              2614 bp    DNA     linear       05-Nov-2024
DEFINITION  {Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPG_L6!}{dblTerm}
ACCESSION   BBa_M10218
VERSION     BBa_M10218.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     CDS             1461..1601
                     /label=BBa_M10049
     misc_feature    1781..1798
                     /label=Linker
     CDS             1799..2471
                     /label=Native N terminus
     CDS             1610..1780
                     /label=Native C terminus
     CDS             1610..2471
                     /label=BBa_M10011
     terminator      2480..2614
                     /label=BBa_K112903
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagag ggcagcggca gtggatctgg cagcggcagt ggatctgtta
     1501 aagaactgga agacaaaaac gaagaactgc tgagtatcat ctaccacctg aaaaacgaag
     1561 ttgctcgtct gaaaaaactg gttggcgaac gtggcggctg ctactagagg atgatattga
     1621 acgtgaaggc catgatttta accgtgtagg tttattttac ggttatgatt tccagaacgg
     1681 actttccgtt tcgctggaat acgcgtttga gtggcaggat cacgacgaag gcgacagtga
     1741 taaattccat tatgcaggtg tcggcgtaaa ttactcgttc ggatctaagt ctcgtcgcga
     1801 ggaaaggaac gactggcact ttaatatcgg cgcgatgtac gaaatagaaa acgtcgaggg
     1861 ttatggcgaa gatatggatg ggctggcgga gccttcagtc tattttaatg ccgccaacgg
     1921 gccgtggaga attgctctgg cctattatca ggaagggccg gtagattata gcgcgggtaa
     1981 acgtggaacg tggtttgatc gcccggagct ggaggtgcat tatcagttcc tcgaaaacga
     2041 tgatttcagt ttcggcctga ccggcggttt ccgtaattat ggttatcact acgttgatga
     2101 accgggtaaa gacacggcga atatgcagcg ctggaaaatc gcgccagact gggatgtgaa
     2161 actgactgac gatttacgtt tcaacggttg gttgtcgatg tataaatttg ccaacgatct
     2221 gaacactacc ggttacgctg atacccgtgt cgaaacggaa acaggtctgc aatatacctt
     2281 caacgaaacg gttgccttgc gagtgaacta ttatcttgag cgcggcttca atatggacga
     2341 cagccgcaat aacggtgagt tttccacgca agaaattcgc gcctatttgc cgctgacgct
     2401 cggcaaccac tcggtgacgc cgtatacgcg cattgggctg gatcgctgga gtaactggga
     2461 ctggcagtaa gtactagagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
     2521 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2581 gctcaccttc gggtgggcct ttctgcgttt atag
//