BBa_M10221 1 BBa_M10221 {Pbad.rbs.prepro.StrepTag}}{&#706;GS5-IILK&#707;}{&#706;Ag43_short!}{dblTerm} 2009-05-09T11:00:00Z 2015-05-08T01:13:52Z BglBricks standard assembly Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a passenger part {GS5-IILK}, Autototansporter {Ag43 short!} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}. {{BioE140LSpr09-AssayOverview}} {{BioE140LSpr09-Growth}} {{BioE140LSpr09-Silver}} false false _271_ 0 4738 271 Not in stock false This composite is incomplete, since we are still missing {GS5-IILK} part = M10049 false Sadao Ota component2002774 1 BBa_K112999 component2002771 1 BBa_K112999 component2002770 1 BBa_M10093 component2002773 1 BBa_M10034 component2002775 1 BBa_K112903 component2002772 1 BBa_K112999 annotation2002774 1 BBa_K112999 range2002774 1 1626 1631 annotation2002773 1 BBa_M10034 range2002773 1 1489 1617 annotation2002770 1 BBa_M10093 range2002770 1 1 1452 annotation2002771 1 BBa_K112999 range2002771 1 1461 1466 annotation2002775 1 BBa_K112903 range2002775 1 1640 1774 annotation2002772 1 BBa_K112999 range2002772 1 1475 1480 BBa_K112903 1 BBa_K112903 b0015 terminator 2008-10-23T11:00:00Z 2015-05-08T01:09:19Z ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_M10034 1 BBa_M10034 {&#706;Ag43_short!}, for Ag 43 autotransporter display system, only transporter 2009-05-09T11:00:00Z 2015-05-08T01:13:51Z The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome. This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at This part is an actual transporter portion of an antigen 43 autotransporter display system based on this reference: PMID: 12107137. false false _271_ 0 4738 271 Not in stock true Bglbricking of M10034, Ag43 (autotransporter only) PCR Oso001 and Oso002 on E. coli MG1655 genomic DNA (937 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, L) Product is pBca9495KC-M10034 {<Ag43_short!} ---------------------------- Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg false Sadao Ota BBa_M10093 1 BBa_M10093 {Pbad.rbs.prepro.StrepTag&#707;} 2009-04-26T11:00:00Z 2015-05-08T01:13:51Z PCR from synthesized DNAs Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar. false false _271_ 0 483 271 Not in stock false N/A false John Anderson annotation2002592 1 Streptavidin Binding Peptide range2002592 1 1374 1412 annotation2002593 1 rbs range2002593 1 1274 1289 annotation2002589 1 AraC range2002589 1 50 805 annotation2002590 1 Pbad range2002590 1 956 1193 annotation2002591 1 PrePro Sequence range2002591 1 1290 1358 BBa_K112903_sequence 1 gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag BBa_M10221_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagagggatcttactagagggatcttactagagggttctggtccgacccagtctcactacggtcagtgcggtggtatcggttactctggtccgaccgtttgcgcttctggtaccacctgccaggttctgaacccgtactactctcagtgcctgggttctggttactagagggatcttactagaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag BBa_K112999_sequence 1 ggatct BBa_M10034_sequence 1 ggttctggtccgacccagtctcactacggtcagtgcggtggtatcggttactctggtccgaccgtttgcgcttctggtaccacctgccaggttctgaacccgtactactctcagtgcctgggttctggt BBa_M10093_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z