BBa_M10221
1
BBa_M10221
{Pbad.rbs.prepro.StrepTag}}{˂GS5-IILK˃}{˂Ag43_short!}{dblTerm}
2009-05-09T11:00:00Z
2015-05-08T01:13:52Z
BglBricks standard assembly
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a passenger part {GS5-IILK}, Autototansporter {Ag43 short!} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}.
{{BioE140LSpr09-AssayOverview}}
{{BioE140LSpr09-Growth}}
{{BioE140LSpr09-Silver}}
false
false
_271_
0
4738
271
Not in stock
false
This composite is incomplete, since we are still missing {GS5-IILK} part = M10049
false
Sadao Ota
component2002774
1
BBa_K112999
component2002771
1
BBa_K112999
component2002770
1
BBa_M10093
component2002773
1
BBa_M10034
component2002775
1
BBa_K112903
component2002772
1
BBa_K112999
annotation2002774
1
BBa_K112999
range2002774
1
1626
1631
annotation2002773
1
BBa_M10034
range2002773
1
1489
1617
annotation2002770
1
BBa_M10093
range2002770
1
1
1452
annotation2002771
1
BBa_K112999
range2002771
1
1461
1466
annotation2002775
1
BBa_K112903
range2002775
1
1640
1774
annotation2002772
1
BBa_K112999
range2002772
1
1475
1480
BBa_K112903
1
BBa_K112903
b0015 terminator
2008-10-23T11:00:00Z
2015-05-08T01:09:19Z
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_M10034
1
BBa_M10034
{˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter
2009-05-09T11:00:00Z
2015-05-08T01:13:51Z
The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome.
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at
This part is an actual transporter portion of an antigen 43 autotransporter display system based on this reference: PMID: 12107137.
false
false
_271_
0
4738
271
Not in stock
true
Bglbricking of M10034, Ag43 (autotransporter only)
PCR Oso001 and Oso002 on E. coli MG1655 genomic DNA (937 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495KC-M10034 {<Ag43_short!}
----------------------------
Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg
Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg
false
Sadao Ota
BBa_M10093
1
BBa_M10093
{Pbad.rbs.prepro.StrepTag˃}
2009-04-26T11:00:00Z
2015-05-08T01:13:51Z
PCR from synthesized DNAs
Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar.
false
false
_271_
0
483
271
Not in stock
false
N/A
false
John Anderson
annotation2002592
1
Streptavidin Binding Peptide
range2002592
1
1374
1412
annotation2002593
1
rbs
range2002593
1
1274
1289
annotation2002589
1
AraC
range2002589
1
50
805
annotation2002590
1
Pbad
range2002590
1
956
1193
annotation2002591
1
PrePro Sequence
range2002591
1
1290
1358
BBa_K112903_sequence
1
gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_M10221_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagagggatcttactagagggatcttactagagggttctggtccgacccagtctcactacggtcagtgcggtggtatcggttactctggtccgaccgtttgcgcttctggtaccacctgccaggttctgaacccgtactactctcagtgcctgggttctggttactagagggatcttactagaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag
BBa_K112999_sequence
1
ggatct
BBa_M10034_sequence
1
ggttctggtccgacccagtctcactacggtcagtgcggtggtatcggttactctggtccgaccgtttgcgcttctggtaccacctgccaggttctgaacccgtactactctcagtgcctgggttctggt
BBa_M10093_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z