LOCUS BBa_M10225 3318 bp DNA linear 05-Nov-2024 DEFINITION {Pbad.rbs.prepro.StrepTag}{˂GS5-IILK˃}{˂TshA!}{dblTe rm} ACCESSION BBa_M10225 VERSION BBa_M10225.1 FEATURES Location/Qualifiers CDS 50..805 /label=AraC promoter 956..1193 /label=Pbad CDS 1290..1358 /label=PrePro Sequence CDS 1374..1412 /label=Streptavidin Binding Peptide RBS 1274..1289 /label=rbs misc_feature 1..1452 /label=BBa_M10093 misc_feature 1453..1458 /label=BBa_K112999 CDS 1459..1599 /label=BBa_M10049 misc_feature 1600..1605 /label=BBa_K112999 CDS 1606..3177 /label=BBa_M10088 misc_feature 3178..3183 /label=BBa_K112999 terminator 3184..3318 /label=BBa_K112903 ORIGIN 1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac 301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc 481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga 1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc 1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca 1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac 1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg 1441 cagtctgggc agggatctgg cagcggcagt ggatctggca gcggcagtgg atctgttaaa 1501 gaactggaag acaaaaacga agaactgctg agtatcatct accacctgaa aaacgaagtt 1561 gctcgtctga aaaaactggt tggcgaacgt ggcggctgcg gatctacact tttacctgta 1621 tacgattatg ccggttcatg gaacctgaag ggagacgatg cccgcctgaa cgtggggccg 1681 tacagtatgt tgtcaggtaa tatcaatgtt caggataaag ggactgtcac cctcggaggg 1741 gaaggggaac tgagtcctga cctgactctt cagaatcaga tgttgtacag cctgtttaac 1801 gggtaccgca atacctggag cgggagcctg aatgcaccgg atgccaccgt cagcatgaca 1861 gacacccagt ggtcgatgaa cggaaactcc acggcaggaa atatgaaact taaccggaca 1921 atagtcggtt ttaacggggg aacatcaccg ttcacgacac tgacaacaga taatctggac 1981 gcggttcagt cagcatttgt catgcgtaca gaccttaaca aggcagacaa actggtgata 2041 aacaagtcgg caacaggtca tgacaacagc atctgggtta acttcctgaa aaaaccttct 2101 aacaaggaca cgcttgatat tccactggtc agcgcacctg aagcgacagc tgataatctg 2161 ttcagggcat caacacgggt tgtgggattc agtgatgtca cccccatcct tagtgtcaga 2221 aaagaggacg ggaaaaaaga gtgggtcctc gatggttacc aggttgcacg taacgacggc 2281 cagggtaagg ctgccgccac attcatgcac atcagctata acaacttcat cactgaagtt 2341 aacaacctga acaaacgcat gggcgatttg agggatatta acggcgaagc cggtacgtgg 2401 gtgcgtctgc tgaacggttc cggctctgct gatggcggtt tcactgacca ctataccctg 2461 ctgcagatgg gggctgaccg taagcacgaa ctgggaagta tggacctgtt taccggcgtg 2521 atggccacct acactgacac agatgcgtca gcagacctgt acagcggtaa aacaaaatca 2581 tggggtggtg gtttctatgc cagtggtctg ttccggtccg gcgcttactt tgatttgatt 2641 gccaaatata ttcacaatga aaacaaatat gacctgaact ttgccggagc tggtaaacag 2701 aacttccgca gccattcact gtatgcaggt gcagaagtcg gataccgtta tcatctgaca 2761 gatacgacgt ttgttgaacc tcaggcggaa ctggtctggg gaagactgca gggccaaaca 2821 tttaactgga acgacagtgg aatggatgtc tcaatgcgtc gtaacagcgt taatcctctg 2881 gtaggcagaa ccggcgttgt ttccggtaaa accttcagtg gtaaggactg gagtctgaca 2941 gcccgtgccg gcctgcatta tgagttcgat ctgacggaca gtgctgacgt tcacctgaag 3001 gatgcagcgg gagaacatca gattaatggc agaaaagacg gtcgtatgct ttacggtgtg 3061 gggttaaatg cccggtttgg cgacaatacg cgtctggggc tggaagttga acgctctgca 3121 ttcggtaaat acaacacaga tgatgcgata aacgctaata ttcgttattc attctgagga 3181 tctgatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3241 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3301 gcctttctgc gtttatag //