BBa_M10049 1 BBa_M10049 {&#706;GS5-IILK&#707;} - Leucine Zipper Peptide IILK 2009-05-09T11:00:00Z 2015-05-08T01:13:51Z pBca1256-mm011 The Leucine Zipper is a motif allowing for the dimerization of two proteins via interactions between two alpha helices. It is found in some transcriptional regulatory proteins. false false _271_ 0 4743 271 Not in stock true Utilized a gateway transfer false Eric Hsieh BBa_M10088 1 BBa_M10088 {&#706;TshA!} | N-terminal displaying Tsh Autotransporter 2009-05-05T11:00:00Z 2015-05-08T01:13:51Z Pubmed nucleotide sequence AF218073 or protein AAA24698 from Escherichia Coli genome. Selected coding sequence includes amino acid 855 to 1358. Autotransporters are mainly used for the translocation of large proteins or protein domains across the outer membrane. The autotransporter protein contains it own machinery, a transporter domain, to secrete its covalently attached, N-terminal passenger across the outer membrane. After secretion to the periplasm through normal SEC dependent pathways, the autotransporter portion inserts into the outer membrane as a Beta-barrel forming oligomeric pores, similar to porins. The covalently linked passenger peptide will pass through this self-formed beta-barrel folding progressively into its native configuration as it reaches the cell surface. The passenger peptide remains in a non-native conformation while it is in the cytoplasm and the periplasm. This part has been successfully used to display... false false _271_ 0 4756 271 Not in stock true This part was design to attach to the C-terminal end of a desired exoprotein passengers, therefore it does not contain a start codon, but does contain a stop codon. Also, it does not include the necessary N-terminal Prepro sequences for SEC secretion across the perplasmic membrane. false David de Renzy BBa_K112903 1 BBa_K112903 b0015 terminator 2008-10-23T11:00:00Z 2015-05-08T01:09:19Z ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_M10225 1 BBa_M10225 {Pbad.rbs.prepro.StrepTag}{&#706;GS5-IILK&#707;}{&#706;TshA!}{dblTerm} 2009-05-09T11:00:00Z 2015-05-08T01:13:52Z BglBrick Standard Assembly GS5-IILK passenger part attached to a Tsh Autotransporter displayer THIS DEVICE IS WRONG!!! WAITING FOR PART BBa_M10049 to register {{BioE140LSpr09-AssayOverview}} {{BioE140LSpr09-Streptavidin}} {{BioE140LSpr09-Growth}} {{BioE140LSpr09-Silver}} false false _271_ 0 4756 271 Not in stock false N/A false David de Renzy component2003017 1 BBa_K112999 component2003013 1 BBa_K112999 component2003012 1 BBa_M10093 component2003015 1 BBa_K112999 component2003018 1 BBa_K112903 component2003014 1 BBa_M10049 component2003016 1 BBa_M10088 annotation2003016 1 BBa_M10088 range2003016 1 1606 3177 annotation2003014 1 BBa_M10049 range2003014 1 1459 1599 annotation2003017 1 BBa_K112999 range2003017 1 3178 3183 annotation2003018 1 BBa_K112903 range2003018 1 3184 3318 annotation2003012 1 BBa_M10093 range2003012 1 1 1452 annotation2003013 1 BBa_K112999 range2003013 1 1453 1458 annotation2003015 1 BBa_K112999 range2003015 1 1600 1605 BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_M10093 1 BBa_M10093 {Pbad.rbs.prepro.StrepTag&#707;} 2009-04-26T11:00:00Z 2015-05-08T01:13:51Z PCR from synthesized DNAs Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar. false false _271_ 0 483 271 Not in stock false N/A false John Anderson annotation2002592 1 Streptavidin Binding Peptide range2002592 1 1374 1412 annotation2002593 1 rbs range2002593 1 1274 1289 annotation2002589 1 AraC range2002589 1 50 805 annotation2002590 1 Pbad range2002590 1 956 1193 annotation2002591 1 PrePro Sequence range2002591 1 1290 1358 BBa_M10049_sequence 1 ggcagcggcagtggatctggcagcggcagtggatctgttaaagaactggaagacaaaaacgaagaactgctgagtatcatctaccacctgaaaaacgaagttgctcgtctgaaaaaactggttggcgaacgtggcggctgc BBa_M10225_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagggatctggcagcggcagtggatctggcagcggcagtggatctgttaaagaactggaagacaaaaacgaagaactgctgagtatcatctaccacctgaaaaacgaagttgctcgtctgaaaaaactggttggcgaacgtggcggctgcggatctacacttttacctgtatacgattatgccggttcatggaacctgaagggagacgatgcccgcctgaacgtggggccgtacagtatgttgtcaggtaatatcaatgttcaggataaagggactgtcaccctcggaggggaaggggaactgagtcctgacctgactcttcagaatcagatgttgtacagcctgtttaacgggtaccgcaatacctggagcgggagcctgaatgcaccggatgccaccgtcagcatgacagacacccagtggtcgatgaacggaaactccacggcaggaaatatgaaacttaaccggacaatagtcggttttaacgggggaacatcaccgttcacgacactgacaacagataatctggacgcggttcagtcagcatttgtcatgcgtacagaccttaacaaggcagacaaactggtgataaacaagtcggcaacaggtcatgacaacagcatctgggttaacttcctgaaaaaaccttctaacaaggacacgcttgatattccactggtcagcgcacctgaagcgacagctgataatctgttcagggcatcaacacgggttgtgggattcagtgatgtcacccccatccttagtgtcagaaaagaggacgggaaaaaagagtgggtcctcgatggttaccaggttgcacgtaacgacggccagggtaaggctgccgccacattcatgcacatcagctataacaacttcatcactgaagttaacaacctgaacaaacgcatgggcgatttgagggatattaacggcgaagccggtacgtgggtgcgtctgctgaacggttccggctctgctgatggcggtttcactgaccactataccctgctgcagatgggggctgaccgtaagcacgaactgggaagtatggacctgtttaccggcgtgatggccacctacactgacacagatgcgtcagcagacctgtacagcggtaaaacaaaatcatggggtggtggtttctatgccagtggtctgttccggtccggcgcttactttgatttgattgccaaatatattcacaatgaaaacaaatatgacctgaactttgccggagctggtaaacagaacttccgcagccattcactgtatgcaggtgcagaagtcggataccgttatcatctgacagatacgacgtttgttgaacctcaggcggaactggtctggggaagactgcagggccaaacatttaactggaacgacagtggaatggatgtctcaatgcgtcgtaacagcgttaatcctctggtaggcagaaccggcgttgtttccggtaaaaccttcagtggtaaggactggagtctgacagcccgtgccggcctgcattatgagttcgatctgacggacagtgctgacgttcacctgaaggatgcagcgggagaacatcagattaatggcagaaaagacggtcgtatgctttacggtgtggggttaaatgcccggtttggcgacaatacgcgtctggggctggaagttgaacgctctgcattcggtaaatacaacacagatgatgcgataaacgctaatattcgttattcattctgaggatctgatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag BBa_K112903_sequence 1 gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag BBa_K112999_sequence 1 ggatct BBa_M10088_sequence 1 acacttttacctgtatacgattatgccggttcatggaacctgaagggagacgatgcccgcctgaacgtggggccgtacagtatgttgtcaggtaatatcaatgttcaggataaagggactgtcaccctcggaggggaaggggaactgagtcctgacctgactcttcagaatcagatgttgtacagcctgtttaacgggtaccgcaatacctggagcgggagcctgaatgcaccggatgccaccgtcagcatgacagacacccagtggtcgatgaacggaaactccacggcaggaaatatgaaacttaaccggacaatagtcggttttaacgggggaacatcaccgttcacgacactgacaacagataatctggacgcggttcagtcagcatttgtcatgcgtacagaccttaacaaggcagacaaactggtgataaacaagtcggcaacaggtcatgacaacagcatctgggttaacttcctgaaaaaaccttctaacaaggacacgcttgatattccactggtcagcgcacctgaagcgacagctgataatctgttcagggcatcaacacgggttgtgggattcagtgatgtcacccccatccttagtgtcagaaaagaggacgggaaaaaagagtgggtcctcgatggttaccaggttgcacgtaacgacggccagggtaaggctgccgccacattcatgcacatcagctataacaacttcatcactgaagttaacaacctgaacaaacgcatgggcgatttgagggatattaacggcgaagccggtacgtgggtgcgtctgctgaacggttccggctctgctgatggcggtttcactgaccactataccctgctgcagatgggggctgaccgtaagcacgaactgggaagtatggacctgtttaccggcgtgatggccacctacactgacacagatgcgtcagcagacctgtacagcggtaaaacaaaatcatggggtggtggtttctatgccagtggtctgttccggtccggcgcttactttgatttgattgccaaatatattcacaatgaaaacaaatatgacctgaactttgccggagctggtaaacagaacttccgcagccattcactgtatgcaggtgcagaagtcggataccgttatcatctgacagatacgacgtttgttgaacctcaggcggaactggtctggggaagactgcagggccaaacatttaactggaacgacagtggaatggatgtctcaatgcgtcgtaacagcgttaatcctctggtaggcagaaccggcgttgtttccggtaaaaccttcagtggtaaggactggagtctgacagcccgtgccggcctgcattatgagttcgatctgacggacagtgctgacgttcacctgaaggatgcagcgggagaacatcagattaatggcagaaaagacggtcgtatgctttacggtgtggggttaaatgcccggtttggcgacaatacgcgtctggggctggaagttgaacgctctgcattcggtaaatacaacacagatgatgcgataaacgctaatattcgttattcattctga BBa_M10093_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z