LOCUS BBa_M1027 5202 bp DNA linear 16-Dec-2024 DEFINITION RBS HlyB RBS HlyD RBS TolC Term ACCESSION BBa_M1027 VERSION BBa_M1027.1 FEATURES Location/Qualifiers RBS 1..14 /label=BBa_B0035 misc_feature 21..23 /label=Start Codon misc_feature 2142..2144 /label=Stop Codon CDS 21..2141 /label=HlyB CDS 21..2144 /label=BBa_M1003 RBS 2153..2166 /label=BBa_B0035 misc_feature 2173..2175 /label=Start Codon CDS 2173..3606 /label=HlyD misc_feature 3607..3609 /label=Stop Codon CDS 2173..3609 /label=BBa_M1004 RBS 3618..3631 /label=BBa_B0035 misc_feature 3638..3640 /label=Start misc_feature 5153..5155 /label=Stop CDS 3638..5152 /label=TolC CDS 3638..5155 /label=BBa_M1028 misc_feature 5164..5202 /label=B1006 stem_loop 5173..5194 /label=modified thr terminator polyA_site 5195..5202 /label=PolyA polyA_site 5164..5172 /label=PolyA terminator 5164..5202 /label=BBa_B1006 ORIGIN 1 attaaagagg agaatactag atggattcct gtcacaagat tgattacggc ctgtatgcac 61 tggaaatcct ggcacaatat cataacgtct ccgtcaaccc ggaggaaatt aaacatcgct 121 tcgatactga cggcactggc ctgggtctga ccagctggct gctggcagcg aagtccctgg 181 agctgaaggt aaagcaagtg aaaaagacga ttgatcgtct gaacttcatc agcctgccgg 241 cactggtttg gcgtgaagat ggccgtcact tcatcctgac gaaagtgtcc aaagaagcga 301 accgctacct gatctttgat ctggagcagc gcaacccgcg cgtactggaa cagagcgagt 361 tcgaggctct gtatcagggc cacatcattc tgattgcatc tcgttcttct gttgcaggta 421 aactggcgaa attcgacttc acctggttca ttccggctat tatcaagtac cgtcgtattt 481 tcatcgaaac cctggttgta tctgttttcc tgcaactgtt cgctctgatt actccgctgt 541 tctttcaggt tgtcatggac aaagttctgg ttcaccgtgg cttttccacc ctgaacgtca 601 ttactgttgc gctgagcgtt gttgttgtgt tcgaaatcat tctgtccggt ctgcgtacct 661 acatcttcgc gcactctacc tcccgcatcg acgttgaact gggtgcgaag ctgttccgtc 721 acctgctggc actgcctatc agctacttcg aatctcgtcg tgtgggcgat actgtggccc 781 gtgttcgtga actggatcag atccgcaact ttctgaccgg ccaggccctg accagcgttc 841 tggatctgct gttcagcttt atcttcttcg cagtaatgtg gtactacagc ccgaagctga 901 ctctggtgat tctgttctct ctgccgtgtt acgcagcttg gtccgtcttc atctctccaa 961 tcctgcgtcg ccgtctggac gacaaattta gccgtaatgc ggataatcag tccttcctgg 1021 tcgaatctgt gactgctatc aacactatca aagccatggc ggtttctcca cagatgacta 1081 acatctggga taagcagctg gcaggttacg ttgctgcggg cttcaaagtg accgttctgg 1141 caaccattgg tcaacagggc attcagctga ttcagaaaac cgtgatgatc attaacctgt 1201 ggctgggtgc gcacctggtc atcagcggtg acctgtccat tggccagctg atcgctttca 1261 acatgctggc gggtcaaatt gttgccccag taatccgtct ggcccagatc tggcaggact 1321 tccagcaggt cggtatttct gtgacccgcc tgggtgacgt cctgaactct ccgaccgaaa 1381 gctaccacgg taaactggct ctgccggaga tcaacggcga tattactttc cgtaacatcc 1441 gttttcgtta caaaccggat tccccggtaa tcctggataa catcaatctg tctatcaaac 1501 agggcgaagt catcggcatc gtgggccgta gcggcagcgg caagtccact ctgactaaac 1561 tgattcagcg cttttacatt ccagaaaacg gtcaggttct gattgacggt cacgacctgg 1621 ctctggcgga cccgaactgg ctgcgtcgtc aggtgggcgt agtgctgcaa gataacgtgc 1681 tgctgaaccg tagcatcatc gataacatca gcctggctaa cccgggcatg tccgtggaaa 1741 aagttatcta tgctgcgaaa ctggcaggcg cgcacgattt catctctgaa ctgcgtgaag 1801 gctacaacac catcgtaggc gaacagggcg ctggcctgtc cggcggtcaa cgtcagcgta 1861 tcgctattgc acgtgcactg gtgaacaacc cgaaaatcct gatcttcgac gaggccactt 1921 ctgctctgga ttacgaatct gaacatatta tcatgcgtaa tatgcacaag atctgcaaag 1981 gtcgcaccgt aatcatcatc gcccaccgtc tgagcaccgt aaagaacgcc gaccgcatca 2041 tcgtgatgga aaaaggtaag atcgttgaac agggtaaaca taaagagctg ctgtccgaac 2101 cggaatctct gtattcttat ctgtaccagc tgcaatctga ttaatactag agattaaaga 2161 ggagaatact agatgaaaac ttggctgatg ggcttctctg agttcctgct gcgttataag 2221 ctggtatgga gcgaaacttg gaaaatccgt aagcaactgg ataccccggt acgtgaaaaa 2281 gacgaaaacg aatttctgcc agcacatctg gaactgattg agacgccggt ttctcgccgt 2341 ccacgtctgg ttgcttactt cattatgggc ttcctggtta ttgctgtaat cctgtctgtt 2401 ctgggtcagg tggaaatcgt tgcgaccgcg aatggtaaac tgaccctgtc cggtcgttct 2461 aaagaaatca aaccgatcga aaactccatc gttaaagaga ttatcgttaa agaaggtgaa 2521 tccgtacgca aaggcgatgt tctgctgaaa ctgaccgcac tgggcgcaga agcggacact 2581 ctgaaaactc agtcctctct gctgcaaacc cgtctggaac agacccgtta ccaaatcctg 2641 agccgttcta ttgaactgaa caaactgccg gaactgaaac tgccggatga accatacttc 2701 cagaacgtaa gcgaggagga agttctgcgc ctgacttccc tgatcaaaga gcagttctct 2761 acttggcaga accagaaata ccaaaaggaa ctgaacctgg acaaaaagcg tgcggagcgc 2821 ctgactatcc tggcccgcat caaccgttac gagaacctgt ctcgtgttga aaaatctcgc 2881 ctggacgact tccgtagcct gctgcacaaa caggccatcg caaaacacgc tgtgctggaa 2941 caggaaaata aatacgtgga agctgccaac gaactgcgtg tatataaaag ccagctggag 3001 cagatcgaga gcgaaatcct gtctgcgaag gaggagtacc agctggtgac gcagctgttc 3061 aaaaatgaaa tcctggacaa actgcgtcaa accacggact ctatcgaact gctgaccctg 3121 gaactggaga aaaacgagga gcgccagcag gccagcgtca tccgcgctcc ggtatctggt 3181 aaagttcagc aactgaaagt tcacaccgaa ggtggcgttg ttaccaccgc agaaaccctg 3241 atggttattg tgccggaaga tgacaccctg gaagttaccg ctctggtgca gaacaaagat 3301 atcggtttca tcaacgtcgg tcagaacgcg atcatcaaag tagaggcttt cccgtacact 3361 cgttacggct acctggtggg caaggtcaaa aacatcaacc tggacgctat cgaagatcag 3421 aaactgggcc tggtgttcaa cgtgatcgtt agcgtagaag agaacgatct gtctaccggt 3481 aacaaacaca ttccactgtc ctccggcatg gcggttactg ctgaaatcaa aaccggtatg 3541 cgtagcgtga tctcctatct gctgtctccg ctggaagaaa gcgtcaccga atctctgcac 3601 gaacgctaat actagagatt aaagaggaga atactagatg attgccctga acactgcctc 3661 gccacagggg atgcagatga agaagctcct gccgatcctg atcgggctaa gtctttccgg 3721 gttcagctca ctttcacaag ccgagaatct tatgcaagta tatcagcagg cacggctgtc 3781 gaatccagag cttcgtaaat cagcggccga tcgagatgct gcgttcgaga aaattaatga 3841 ggcccggtca ccgctgttac cacagctggg tctcggcgcg gattatacat acagcaacgg 3901 ctaccgtgat gcgaatggaa taaatagtaa cgcaaccagc gcaagcttac aactgacaca 3961 gtcgatcttc gatatgtcga aatggcgcgc actaacgctt caggaaaaag cagccgggat 4021 ccaagatgta acttatcaga ccgaccagca aacattgatt ttgaatactg caaccgctta 4081 ctttaacgtt ctgaatgcca tcgacgtgct gagctacacc caggcacaaa aggaagccat 4141 ttatcgtcaa cttgaccaga ctacgcagcg gtttaacgtt gggttagtag caatcaccga 4201 tgttcaaaat gcgcgtgcgc agtatgatac cgttctggcc aatgaggtca ccgcacgcaa 4261 caatcttgat aatgcggttg aacagttacg tcagattacc gggaactatt atcccgagct 4321 ggcagcgctg aatgtggaaa attttaaaac tgacaaacct caacctgtca ataccctgct 4381 caaggaagcc gagaaacgca acctgtctct attgcaagct cgcctgtctc aagacctggc 4441 tcgggagcaa atacgccagg cacaggacgg acatctgccc acactggacc tgaccgcaag 4501 tactgggata tccgacacca gttactctgg atcaaaaact cgtggcgccg ccggaacaca 4561 atacgatgac tcaaatatgg gccagaacaa ggttggcctg tccttcagtt taccgatcta 4621 ccagggcggg atggtgaata gtcaagtaaa acaggcgcag tataactttg tcggtgcatc 4681 agagcaactg gaatcagctc accgtagcgt cgtacagact gtccggagta gctttaacaa 4741 cattaacgcg agtatctcat caatcaatgc ttacaagcaa gcagtggttt ctgcgcagtc 4801 ttctttggat gccatggaag caggctattc agtgggtact cgcacgattg ttgatgttct 4861 cgacgctacc acaactctct acaacgcgaa acaggaatta gcgaatgccc gttataacta 4921 cttaattaac caactgaata tcaaatctgc cctaggtact ctgaatgagc aagacttgct 4981 tgcgttaaat aacgccctgt ccaaacctgt ctcaaccaat cctgagaacg tagcacctca 5041 gacccctgaa cagaacgcca ttgcggacgg ttacgcccct gacagtcctg cgcctgtggt 5101 ccagcagacg tcagcgcgca ctaccacttc aaacgggcat aatccgttcc gcaattacta 5161 gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt tt //