BBa_M1028
1
BBa_M1028
tolC-Outer membrane protein
2011-04-25T11:00:00Z
2015-05-08T01:13:52Z
E. coli genome, UTI89
TolC is an outer membrane protein that is essential in the type I secretion pathway together with membrane proteins HlyB and HlyD.
false
false
_768_
0
8961
9
Not in stock
false
Sequence was modified to remove incompatible PstI sites as well as to avoid the presence of any of the Zn finger binding sequences for the Zif268-HIV, Zif23-GCN4, and Blues Zn fingers.
false
Alex Hatch
annotation2117428
1
TolC
range2117428
1
1
1515
annotation2117426
1
Start
range2117426
1
1
3
annotation2117427
1
Stop
range2117427
1
1516
1518
BBa_M1027
1
BBa_M1027
RBS HlyB RBS HlyD RBS TolC Term
2011-04-19T11:00:00Z
2015-05-08T01:13:52Z
See Subparts
Codes for the HlyB and HlyD membrane proteins necessary for Type I secretion.
false
false
_768_
0
8961
9
Not in stock
false
None
false
Alex Hatch
component2117590
1
BBa_B1006
component2117575
1
BBa_M1003
component2117580
1
BBa_M1004
component2117585
1
BBa_M1028
component2117571
1
BBa_B0035
component2117581
1
BBa_B0035
component2117576
1
BBa_B0035
annotation2117581
1
BBa_B0035
range2117581
1
3618
3631
annotation2117571
1
BBa_B0035
range2117571
1
1
14
annotation2117585
1
BBa_M1028
range2117585
1
3638
5155
annotation2117580
1
BBa_M1004
range2117580
1
2173
3609
annotation2117590
1
BBa_B1006
range2117590
1
5164
5202
annotation2117576
1
BBa_B0035
range2117576
1
2153
2166
annotation2117575
1
BBa_M1003
range2117575
1
21
2144
BBa_M1004
1
BBa_M1004
HlyD Membrane Protein
2011-04-12T11:00:00Z
2015-05-08T01:13:51Z
Genomic. Escherichia coli UTI89
Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
false
false
_768_
0
8961
9
Not in stock
false
Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
false
Alex Hatch
annotation2115953
1
HlyD
range2115953
1
1
1434
annotation2115952
1
Start Codon
range2115952
1
1
3
annotation2115954
1
Stop Codon
range2115954
1
1435
1437
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898429
1
modified thr terminator
range1898429
1
10
31
BBa_B0035
1
BBa_B0035
RBS (B0030 derivative)
2004-01-27T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
false
_1_
0
24
7
Not in stock
false
false
Jason Kelly
BBa_M1003
1
BBa_M1003
HlyB membrane protein
2011-04-12T11:00:00Z
2015-05-08T01:13:51Z
Genomic. Escherichia coli UT189
Membrane protein involved in type I secretion.
false
false
_768_
0
8961
9
Not in stock
false
Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
false
Alex Hatch
annotation2115950
1
Stop Codon
range2115950
1
2122
2124
annotation2115951
1
HlyB
range2115951
1
1
2121
annotation2115949
1
Start Codon
range2115949
1
1
3
BBa_M1004_sequence
1
atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
BBa_B0035_sequence
1
attaaagaggagaa
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_M1027_sequence
1
attaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagattaaagaggagaatactagatgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_M1003_sequence
1
atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaa
BBa_M1028_sequence
1
atgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z