Total construct Pha, Phb, Phc plus zinc fingers and promoter
This is the entire construct of the DNA to be inserted into the plasmid and put into E. Coli. All Pha, Phb, and Phc enzymes are silver fusion compatible to their respective Zinc fingers.
RBS+"Blues" Zinc Finger+ phaC+ terminator
"Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. The designed part is compatible with the Silver fusion assembly standard. The codons were optimized for expression on E. coli.
See existing parts BBa_K165006, BBa_K156012, and BBa_B0035
This composite part contains the ribosome binding site BBa_B0035 a binding domain and the coding region that encodes for the PhaA pathway. The binding domain that was used is the zif268 HIV zn finger. This domain binds to the sequence GAT GCT GCA. The last coding region that is in this composite part is the PhaA pathway that encodes 1/3 of the enzymes necessary to produce bioplastic. This part is silver fusion compatible.
Silver Fusion Compatible PhaB gene with zif23-GCN4
The bioplastic enzyme PhaB has been combined with a zinc finger to increase binding. Also this sequence is silver fusion compatible and has a scar in between the Zif23-GCN4 and the PhaB enzyme. The optimal RBS has been attached at the beginning of the sequence
This sequence is supposed to be silver fusion compatible.
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
phaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)
phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993)
PhaC: A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene
"Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs.
The coding sequences were fused using the Silver fusion standard. Both sequences are optimized for expression in E. coli
Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)
Protein Engineering Gods from the Pabo Lab
Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.
This protein should form a dimer and bind a specific operator site, thus repressing transcription.
Conversion of the iGEM parts registry to SBOL2.1