BBa_M1030
type: ComponentDefinition
persistent identity: BBa M1030
display id: BBa_M1030
version: 1
was generated by: iGEM to SBOL conversion
description: Total construct Pha, Phb, Phc plus zinc fingers and promoter
created: 2011-04-26T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: Parts registry
owned by: james
owned by: myers
mutable description: This is the entire construct of the DNA to be inserted into the plasmid and put into E. Coli. All Pha, Phb, and Phc enzymes are silver fusion compatible to their respective Zinc fingers.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8955
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is silver fusion compatible
star: false
creator: Neil Draper
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1023
component: BBa_M1019
component: BBa_M1024
component: BBa_I14018
sequence annotation: BBa_I14018
sequence annotation: BBa_M1024
sequence annotation: BBa_M1019
sequence annotation: BBa_M1023
sequence: https://synbiohub.org/public/igem/BBa_M1030_sequence/1

BBa_M1023
type: Component
persistent identity: component2289136
display id: component2289136
version: 1
top level: BBa_M1030
definition: BBa_M1023
access: public

BBa_M1019
type: Component
persistent identity: component2289121
display id: component2289121
version: 1
top level: BBa_M1030
definition: BBa_M1019
access: public

BBa_M1024
type: Component
persistent identity: component2289105
display id: component2289105
version: 1
top level: BBa_M1030
definition: BBa_M1024
access: public

BBa_I14018
type: Component
persistent identity: component2289098
display id: component2289098
version: 1
top level: BBa_M1030
definition: Bla
access: public

BBa_I14018
type: SequenceAnnotation
persistent identity: annotation2289098
display id: annotation2289098
version: 1
top level: BBa_M1030
location: https://synbiohub.org/public/igem/BBa_M1030/annotation2289098/range2289098/1
component: BBa_I14018

https://synbiohub.org/public/igem/BBa_M1030/annotation2289098/range2289098/1
type: Range
persistent identity: range2289098
display id: range2289098
version: 1
top level: BBa_M1030
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_M1024
type: SequenceAnnotation
persistent identity: annotation2289105
display id: annotation2289105
version: 1
top level: BBa_M1030
location: https://synbiohub.org/public/igem/BBa_M1030/annotation2289105/range2289105/1
component: BBa_M1024

https://synbiohub.org/public/igem/BBa_M1030/annotation2289105/range2289105/1
type: Range
persistent identity: range2289105
display id: range2289105
version: 1
top level: BBa_M1030
direction: direction/reverse
start: 44
end: 1556
orientation: inline

BBa_M1019
type: SequenceAnnotation
persistent identity: annotation2289121
display id: annotation2289121
version: 1
top level: BBa_M1030
location: https://synbiohub.org/public/igem/BBa_M1030/annotation2289121/range2289121/1
component: BBa_M1019

https://synbiohub.org/public/igem/BBa_M1030/annotation2289121/range2289121/1
type: Range
persistent identity: range2289121
display id: range2289121
version: 1
top level: BBa_M1030
direction: direction/reverse
start: 1565
end: 2641
orientation: inline

BBa_M1023
type: SequenceAnnotation
persistent identity: annotation2289136
display id: annotation2289136
version: 1
top level: BBa_M1030
location: https://synbiohub.org/public/igem/BBa_M1030/annotation2289136/range2289136/1
component: BBa_M1023

https://synbiohub.org/public/igem/BBa_M1030/annotation2289136/range2289136/1
type: Range
persistent identity: range2289136
display id: range2289136
version: 1
top level: BBa_M1030
direction: direction/reverse
start: 2650
end: 4796
orientation: inline

BBa_M1023
type: ComponentDefinition
persistent identity: BBa M1023
display id: BBa_M1023
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+"Blues" Zinc Finger+ phaC+ terminator
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. The designed part is compatible with the Silver fusion assembly standard. The codons were optimized for expression on E. coli.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8986
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Miguel Suastegui
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1021
component: BBa_B1001
sequence annotation: BBa_M1021
sequence annotation: BBa_B1001
sequence: https://synbiohub.org/public/igem/BBa_M1023_sequence/1

BBa_M1021
type: Component
persistent identity: component2117231
display id: component2117231
version: 1
top level: BBa_M1023
definition: BBa_M1021
access: public

BBa_B1001
type: Component
persistent identity: component2117236
display id: component2117236
version: 1
top level: BBa_M1023
definition: BBa_B1001
access: public

BBa_M1021
type: SequenceAnnotation
persistent identity: annotation2117231
display id: annotation2117231
version: 1
top level: BBa_M1023
location: https://synbiohub.org/public/igem/BBa_M1023/annotation2117231/range2117231/1
component: BBa_M1021

https://synbiohub.org/public/igem/BBa_M1023/annotation2117231/range2117231/1
type: Range
persistent identity: range2117231
display id: range2117231
version: 1
top level: BBa_M1023
direction: direction/reverse
start: 1
end: 2105
orientation: inline

BBa_B1001
type: SequenceAnnotation
persistent identity: annotation2117236
display id: annotation2117236
version: 1
top level: BBa_M1023
location: https://synbiohub.org/public/igem/BBa_M1023/annotation2117236/range2117236/1
component: BBa_B1001

https://synbiohub.org/public/igem/BBa_M1023/annotation2117236/range2117236/1
type: Range
persistent identity: range2117236
display id: range2117236
version: 1
top level: BBa_M1023
direction: direction/reverse
start: 2114
end: 2147
orientation: inline

BBa_M1024
type: ComponentDefinition
persistent identity: BBa M1024
display id: BBa_M1024
version: 1
was generated by: iGEM to SBOL conversion
description: RBS and HIV Zn finger and PhaA pathway
created: 2011-04-17T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: See existing parts BBa_K165006, BBa_K156012, and BBa_B0035
owned by: james
owned by: myers
mutable description: This composite part contains the ribosome binding site BBa_B0035 a binding domain and the coding region that encodes for the PhaA pathway. The binding domain that was used is the zif268 HIV zn finger. This domain binds to the sequence GAT GCT GCA. The last coding region that is in this composite part is the PhaA pathway that encodes 1/3 of the enzymes necessary to produce bioplastic. This part is silver fusion compatible.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8955
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Neil Draper
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0035
component: BBa_K125501
component: BBa_K165006
sequence annotation: BBa_K165006
sequence annotation: BBa_K125501
sequence annotation: BBa_B0035
sequence: https://synbiohub.org/public/igem/BBa_M1024_sequence/1

BBa_B0035
type: Component
persistent identity: component2116129
display id: component2116129
version: 1
top level: BBa_M1024
definition: BBa_B0035
access: public

BBa_K125501
type: Component
persistent identity: component2116128
display id: component2116128
version: 1
top level: BBa_M1024
definition: BBa_K125501
access: public

BBa_K165006
type: Component
persistent identity: component2116124
display id: component2116124
version: 1
top level: BBa_M1024
definition: BBa_K165006
access: public

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2116124
display id: annotation2116124
version: 1
top level: BBa_M1024
location: https://synbiohub.org/public/igem/BBa_M1024/annotation2116124/range2116124/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_M1024/annotation2116124/range2116124/1
type: Range
persistent identity: range2116124
display id: range2116124
version: 1
top level: BBa_M1024
direction: direction/reverse
start: 1
end: 252
orientation: inline

BBa_K125501
type: SequenceAnnotation
persistent identity: annotation2116128
display id: annotation2116128
version: 1
top level: BBa_M1024
location: https://synbiohub.org/public/igem/BBa_M1024/annotation2116128/range2116128/1
component: BBa_K125501

https://synbiohub.org/public/igem/BBa_M1024/annotation2116128/range2116128/1
type: Range
persistent identity: range2116128
display id: range2116128
version: 1
top level: BBa_M1024
direction: direction/reverse
start: 259
end: 1491
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2116129
display id: annotation2116129
version: 1
top level: BBa_M1024
location: https://synbiohub.org/public/igem/BBa_M1024/annotation2116129/range2116129/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1024/annotation2116129/range2116129/1
type: Range
persistent identity: range2116129
display id: range2116129
version: 1
top level: BBa_M1024
direction: direction/reverse
start: 1500
end: 1513
orientation: inline

BBa_M1019
type: ComponentDefinition
persistent identity: BBa M1019
display id: BBa_M1019
version: 1
was generated by: iGEM to SBOL conversion
description: Silver Fusion Compatible PhaB gene with zif23-GCN4
created: 2011-04-18T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: The bioplastic enzyme PhaB has been combined with a zinc finger to increase binding. Also this sequence is silver fusion compatible and has a scar in between the Zif23-GCN4 and the PhaB enzyme. The optimal RBS has been attached at the beginning of the sequence
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8965
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence is supposed to be silver fusion compatible.
star: false
creator: Michaela Hugie
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K125502
component: BBa_C2001
component: BBa_B0035
sequence annotation: BBa_B0035
sequence annotation: BBa_C2001
sequence annotation: BBa_K125502
sequence: https://synbiohub.org/public/igem/BBa_M1019_sequence/1

BBa_K125502
type: Component
persistent identity: component2247704
display id: component2247704
version: 1
top level: BBa_M1019
definition: BBa_K125502
access: public

BBa_C2001
type: Component
persistent identity: component2247700
display id: component2247700
version: 1
top level: BBa_M1019
definition: zif23
access: public

BBa_B0035
type: Component
persistent identity: component2247690
display id: component2247690
version: 1
top level: BBa_M1019
definition: BBa_B0035
access: public

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2247690
display id: annotation2247690
version: 1
top level: BBa_M1019
location: https://synbiohub.org/public/igem/BBa_M1019/annotation2247690/range2247690/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1019/annotation2247690/range2247690/1
type: Range
persistent identity: range2247690
display id: range2247690
version: 1
top level: BBa_M1019
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_C2001
type: SequenceAnnotation
persistent identity: annotation2247700
display id: annotation2247700
version: 1
top level: BBa_M1019
location: https://synbiohub.org/public/igem/BBa_M1019/annotation2247700/range2247700/1
component: BBa_C2001

https://synbiohub.org/public/igem/BBa_M1019/annotation2247700/range2247700/1
type: Range
persistent identity: range2247700
display id: range2247700
version: 1
top level: BBa_M1019
direction: direction/reverse
start: 21
end: 345
orientation: inline

BBa_K125502
type: SequenceAnnotation
persistent identity: annotation2247704
display id: annotation2247704
version: 1
top level: BBa_M1019
location: https://synbiohub.org/public/igem/BBa_M1019/annotation2247704/range2247704/1
component: BBa_K125502

https://synbiohub.org/public/igem/BBa_M1019/annotation2247704/range2247704/1
type: Range
persistent identity: range2247704
display id: range2247704
version: 1
top level: BBa_M1019
direction: direction/reverse
start: 352
end: 1077
orientation: inline

Bla
type: ComponentDefinition
persistent identity: BBa I14018
display id: BBa_I14018
version: 1
was generated by: iGEM to SBOL conversion
description: P(Bla)
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pUC19
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: P(Bla), Medium Transcription
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Bla)
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14018_sequence/1

P(Bla)
type: SequenceAnnotation
persistent identity: annotation1018027
display id: annotation1018027
version: 1
top level: Bla
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1
type: Range
persistent identity: range1018027
display id: range1018027
version: 1
top level: Bla
direction: direction/forward
start: 1
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1027020
display id: annotation1027020
version: 1
top level: Bla
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1
type: Range
persistent identity: range1027020
display id: range1027020
version: 1
top level: Bla
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1027016
display id: annotation1027016
version: 1
top level: Bla
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1
type: Range
persistent identity: range1027016
display id: range1027016
version: 1
top level: Bla
direction: direction/forward
start: 25
end: 30
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_K125501
type: ComponentDefinition
persistent identity: BBa K125501
display id: BBa_K125501
version: 1
was generated by: iGEM to SBOL conversion
description: phaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
mutable description: phaA
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PHB synthesis pathway
star: false
creator: Norman Wang
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: mt tGa to tAa
sequence annotation: inserted second stop
sequence: https://synbiohub.org/public/igem/BBa_K125501_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1971707
display id: annotation1971707
version: 1
top level: BBa_K125501
location: https://synbiohub.org/public/igem/BBa_K125501/annotation1971707/range1971707/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K125501/annotation1971707/range1971707/1
type: Range
persistent identity: range1971707
display id: range1971707
version: 1
top level: BBa_K125501
direction: direction/forward
start: 1
end: 3
orientation: inline

mt tGa to tAa
type: SequenceAnnotation
persistent identity: annotation1971705
display id: annotation1971705
version: 1
top level: BBa_K125501
location: https://synbiohub.org/public/igem/BBa_K125501/annotation1971705/range1971705/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K125501/annotation1971705/range1971705/1
type: Range
persistent identity: range1971705
display id: range1971705
version: 1
top level: BBa_K125501
direction: direction/forward
start: 1228
end: 1230
orientation: inline

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971706
display id: annotation1971706
version: 1
top level: BBa_K125501
location: https://synbiohub.org/public/igem/BBa_K125501/annotation1971706/range1971706/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K125501/annotation1971706/range1971706/1
type: Range
persistent identity: range1971706
display id: range1971706
version: 1
top level: BBa_K125501
direction: direction/forward
start: 1231
end: 1233
orientation: inline

BBa_K125502
type: ComponentDefinition
persistent identity: BBa K125502
display id: BBa_K125502
version: 1
was generated by: iGEM to SBOL conversion
description: phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993)
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
mutable description: phaB
discontinued: false
dominant: false
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PHB biosynthesis pathway
star: false
creator: Norman Wang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: original stop codon
sequence annotation: inserted second stop
sequence: https://synbiohub.org/public/igem/BBa_K125502_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1971708
display id: annotation1971708
version: 1
top level: BBa_K125502
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971708/range1971708/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K125502/annotation1971708/range1971708/1
type: Range
persistent identity: range1971708
display id: range1971708
version: 1
top level: BBa_K125502
direction: direction/forward
start: 1
end: 3
orientation: inline

original stop codon
type: SequenceAnnotation
persistent identity: annotation1971709
display id: annotation1971709
version: 1
top level: BBa_K125502
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971709/range1971709/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K125502/annotation1971709/range1971709/1
type: Range
persistent identity: range1971709
display id: range1971709
version: 1
top level: BBa_K125502
direction: direction/forward
start: 721
end: 723
orientation: inline

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971710
display id: annotation1971710
version: 1
top level: BBa_K125502
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971710/range1971710/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K125502/annotation1971710/range1971710/1
type: Range
persistent identity: range1971710
display id: range1971710
version: 1
top level: BBa_K125502
direction: direction/forward
start: 724
end: 726
orientation: inline

BBa_K165006
type: ComponentDefinition
persistent identity: BBa K165006
display id: BBa_K165006
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV DNA-binding domain
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K165006_sequence/1

BBa_M1021
type: ComponentDefinition
persistent identity: BBa M1021
display id: BBa_M1021
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+ "Blues" Zinc Finger + PhaC
created: 2011-04-11T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: PhaC: A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene
owned by: james
owned by: myers
mutable description: "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8986
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The coding sequences were fused using the Silver fusion standard. Both sequences are optimized for expression in E. coli
star: false
creator: Miguel Suastegui
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1020
component: BBa_B0035
sequence annotation: BBa_B0035
sequence annotation: BBa_M1020
sequence: https://synbiohub.org/public/igem/BBa_M1021_sequence/1

BBa_M1020
type: Component
persistent identity: component2117222
display id: component2117222
version: 1
top level: BBa_M1021
definition: BBa_M1020
access: public

BBa_B0035
type: Component
persistent identity: component2117215
display id: component2117215
version: 1
top level: BBa_M1021
definition: BBa_B0035
access: public

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117215
display id: annotation2117215
version: 1
top level: BBa_M1021
location: https://synbiohub.org/public/igem/BBa_M1021/annotation2117215/range2117215/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1021/annotation2117215/range2117215/1
type: Range
persistent identity: range2117215
display id: range2117215
version: 1
top level: BBa_M1021
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_M1020
type: SequenceAnnotation
persistent identity: annotation2117222
display id: annotation2117222
version: 1
top level: BBa_M1021
location: https://synbiohub.org/public/igem/BBa_M1021/annotation2117222/range2117222/1
component: BBa_M1020

https://synbiohub.org/public/igem/BBa_M1021/annotation2117222/range2117222/1
type: Range
persistent identity: range2117222
display id: range2117222
version: 1
top level: BBa_M1021
direction: direction/reverse
start: 15
end: 2105
orientation: inline

zif23
type: ComponentDefinition
persistent identity: BBa C2001
display id: BBa_C2001
version: 1
was generated by: iGEM to SBOL conversion
description: Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Protein Engineering Gods from the Pabo Lab
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This protein should form a dimer and bind a specific operator site, thus repressing transcription.
star: false
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Finger 2
sequence annotation: Recognition site
sequence annotation: Finger 3
sequence annotation: linker
sequence annotation: GCN4: leucine zipper
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C2001_sequence/1

Finger 2
type: SequenceAnnotation
persistent identity: annotation318101
display id: annotation318101
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1
type: Range
persistent identity: range318101
display id: range318101
version: 1
top level: zif23
direction: direction/forward
start: 4
end: 90
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318108
display id: annotation318108
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1
type: Range
persistent identity: range318108
display id: range318108
version: 1
top level: zif23
direction: direction/forward
start: 43
end: 63
orientation: inline

Finger 3
type: SequenceAnnotation
persistent identity: annotation318103
display id: annotation318103
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1
type: Range
persistent identity: range318103
display id: range318103
version: 1
top level: zif23
direction: direction/forward
start: 91
end: 150
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318109
display id: annotation318109
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1
type: Range
persistent identity: range318109
display id: range318109
version: 1
top level: zif23
direction: direction/forward
start: 127
end: 147
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation318104
display id: annotation318104
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1
type: Range
persistent identity: range318104
display id: range318104
version: 1
top level: zif23
direction: direction/forward
start: 151
end: 165
orientation: inline

GCN4: leucine zipper
type: SequenceAnnotation
persistent identity: annotation318105
display id: annotation318105
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1
type: Range
persistent identity: range318105
display id: range318105
version: 1
top level: zif23
direction: direction/forward
start: 166
end: 261
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation318106
display id: annotation318106
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1
type: Range
persistent identity: range318106
display id: range318106
version: 1
top level: zif23
direction: direction/forward
start: 262
end: 294
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation318107
display id: annotation318107
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1
type: Range
persistent identity: range318107
display id: range318107
version: 1
top level: zif23
direction: direction/forward
start: 295
end: 300
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214011
display id: annotation2214011
version: 1
top level: zif23
location: https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1
type: Range
persistent identity: range2214011
display id: range2214011
version: 1
top level: zif23
direction: direction/forward
start: 301
end: 325
orientation: inline

BBa_B1001
type: ComponentDefinition
persistent identity: BBa B1001
display id: BBa_B1001
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artifical, small, %T~=90)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artificial terminator, estimated %T>90
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Poly T tail of 9 residues for high %T
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1001
sequence annotation: PolyA tail
sequence annotation: stem loop
sequence annotation: Poly A tail
sequence: https://synbiohub.org/public/igem/BBa_B1001_sequence/1

B1001
type: SequenceAnnotation
persistent identity: annotation1898382
display id: annotation1898382
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
type: Range
persistent identity: range1898382
display id: range1898382
version: 1
top level: BBa_B1001
direction: direction/forward
start: 1
end: 34
orientation: inline

PolyA tail
type: SequenceAnnotation
persistent identity: annotation1898381
display id: annotation1898381
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
type: Range
persistent identity: range1898381
display id: range1898381
version: 1
top level: BBa_B1001
direction: direction/reverse
start: 1
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898379
display id: annotation1898379
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
type: Range
persistent identity: range1898379
display id: range1898379
version: 1
top level: BBa_B1001
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898380
display id: annotation1898380
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
type: Range
persistent identity: range1898380
display id: range1898380
version: 1
top level: BBa_B1001
direction: direction/reverse
start: 26
end: 34
orientation: inline

BBa_M1020
type: ComponentDefinition
persistent identity: BBa M1020
display id: BBa_M1020
version: 1
was generated by: iGEM to SBOL conversion
description: "Blues" Zinc Finger + PhaC
created: 2011-04-11T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: PhaC: A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene
owned by: james
owned by: myers
mutable description: "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8986
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The coding sequences were fused using the Silver fusion standard. Both sequences are optimized for expression in E. coli
star: false
creator: Miguel Suastegui
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Blues Zinc Finger
sequence annotation: start
sequence annotation: Silver fusion Scar
sequence annotation: phaC gene
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M1020_sequence/1

Blues Zinc Finger
type: SequenceAnnotation
persistent identity: annotation2115849
display id: annotation2115849
version: 1
top level: BBa_M1020
location: https://synbiohub.org/public/igem/BBa_M1020/annotation2115849/range2115849/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1020/annotation2115849/range2115849/1
type: Range
persistent identity: range2115849
display id: range2115849
version: 1
top level: BBa_M1020
direction: direction/forward
start: 1
end: 315
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2115850
display id: annotation2115850
version: 1
top level: BBa_M1020
location: https://synbiohub.org/public/igem/BBa_M1020/annotation2115850/range2115850/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1020/annotation2115850/range2115850/1
type: Range
persistent identity: range2115850
display id: range2115850
version: 1
top level: BBa_M1020
direction: direction/forward
start: 1
end: 3
orientation: inline

Silver fusion Scar
type: SequenceAnnotation
persistent identity: annotation2115851
display id: annotation2115851
version: 1
top level: BBa_M1020
location: https://synbiohub.org/public/igem/BBa_M1020/annotation2115851/range2115851/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1020/annotation2115851/range2115851/1
type: Range
persistent identity: range2115851
display id: range2115851
version: 1
top level: BBa_M1020
direction: direction/forward
start: 316
end: 321
orientation: inline

phaC gene
type: SequenceAnnotation
persistent identity: annotation2115852
display id: annotation2115852
version: 1
top level: BBa_M1020
location: https://synbiohub.org/public/igem/BBa_M1020/annotation2115852/range2115852/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1020/annotation2115852/range2115852/1
type: Range
persistent identity: range2115852
display id: range2115852
version: 1
top level: BBa_M1020
direction: direction/forward
start: 322
end: 2091
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2115853
display id: annotation2115853
version: 1
top level: BBa_M1020
location: https://synbiohub.org/public/igem/BBa_M1020/annotation2115853/range2115853/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1020/annotation2115853/range2115853/1
type: Range
persistent identity: range2115853
display id: range2115853
version: 1
top level: BBa_M1020
direction: direction/forward
start: 322
end: 324
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2115854
display id: annotation2115854
version: 1
top level: BBa_M1020
location: https://synbiohub.org/public/igem/BBa_M1020/annotation2115854/range2115854/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1020/annotation2115854/range2115854/1
type: Range
persistent identity: range2115854
display id: range2115854
version: 1
top level: BBa_M1020
direction: direction/forward
start: 2089
end: 2091
orientation: inline

https://synbiohub.org/public/igem/BBa_M1030_sequence/1
type: Sequence
persistent identity: BBa M1030 sequence
display id: BBa_M1030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtaagtttatacataggcgagtactctgttatggtactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagatggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaactcccctgggacgttttggtggctccctgaccaatttttcggcggcggatttgggagcccatgtgatgaaaagcgtcctagcccaggccggggtggggggagaccagcttgacctgtacattatgggcaacgtgttaagggctggccatgggcaattgattccccggcaagcggccttgaaggcggagattcccgatacggtggacggttatgcagtggatatggtctgctcttccgccatgatgagtgtgattaatgcagctttaaccatccgggctggggagggggatttaattttagctggagggacggaatccatgtcccaaactggtttttacctttcccaccgggcccgctggggctacaagttcctcatgggagcaccggaaaatttaaccgatctcctgctccatgatggtttgacggacagcaccaatggggagggcatgggggaacagacggaaaaattggccgcagagcatggtttcagccggatagaattggacgaagtggcctgcttatcgcaacaaagggcggcccacgccacggaatctggttattttgactcagaaattgccccgattgaaatcaccagtcggaaaggaacccaggtgttagccagtgatgaaggtattcgcagtgacaccaccgtggaaagtctaggcaaattgcgctcggcctttgccaaggacggagtgttaacagcgggcaactgtagtcagattaccgatggggcggcagctttgctcctggccagtggggaagcggtggaaaaatatcaactcaaacccttggcaaaaattttgggaggcagttgggcggcgggtactcccagtcgttttcctgagttgcctattaccgctagccagaaactgttagcaaagctagataaaaccctggcagattttgacttgtttgaaaataatgaagccttctccgtcagcaatcttttatttgagcggcggttgggggtggatcgggacaagttgaatgtgaacggtggggcgatcgccctggggcatcccattggagcgtcgggggccaggatcatggttaccctactctatgccttgcaacaacgggataaaactctggggttagcagccctttgccatggcacggggggcggtacggcgatcgccttagagcgggtgtaataatactagagattaaagaggagaatactagagattaaagaggagaatactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1023_sequence/1
type: Sequence
persistent identity: BBa M1023 sequence
display id: BBa_M1023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1024_sequence/1
type: Sequence
persistent identity: BBa M1024 sequence
display id: BBa_M1024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagatggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaactcccctgggacgttttggtggctccctgaccaatttttcggcggcggatttgggagcccatgtgatgaaaagcgtcctagcccaggccggggtggggggagaccagcttgacctgtacattatgggcaacgtgttaagggctggccatgggcaattgattccccggcaagcggccttgaaggcggagattcccgatacggtggacggttatgcagtggatatggtctgctcttccgccatgatgagtgtgattaatgcagctttaaccatccgggctggggagggggatttaattttagctggagggacggaatccatgtcccaaactggtttttacctttcccaccgggcccgctggggctacaagttcctcatgggagcaccggaaaatttaaccgatctcctgctccatgatggtttgacggacagcaccaatggggagggcatgggggaacagacggaaaaattggccgcagagcatggtttcagccggatagaattggacgaagtggcctgcttatcgcaacaaagggcggcccacgccacggaatctggttattttgactcagaaattgccccgattgaaatcaccagtcggaaaggaacccaggtgttagccagtgatgaaggtattcgcagtgacaccaccgtggaaagtctaggcaaattgcgctcggcctttgccaaggacggagtgttaacagcgggcaactgtagtcagattaccgatggggcggcagctttgctcctggccagtggggaagcggtggaaaaatatcaactcaaacccttggcaaaaattttgggaggcagttgggcggcgggtactcccagtcgttttcctgagttgcctattaccgctagccagaaactgttagcaaagctagataaaaccctggcagattttgacttgtttgaaaataatgaagccttctccgtcagcaatcttttatttgagcggcggttgggggtggatcgggacaagttgaatgtgaacggtggggcgatcgccctggggcatcccattggagcgtcgggggccaggatcatggttaccctactctatgccttgcaacaacgggataaaactctggggttagcagccctttgccatggcacggggggcggtacggcgatcgccttagagcgggtgtaataatactagagattaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1019_sequence/1
type: Sequence
persistent identity: BBa M1019 sequence
display id: BBa_M1019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaatactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14018_sequence/1
type: Sequence
persistent identity: BBa I14018 sequence
display id: BBa_I14018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtaagtttatacataggcgagtactctgttatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125501_sequence/1
type: Sequence
persistent identity: BBa K125501 sequence
display id: BBa_K125501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaactcccctgggacgttttggtggctccctgaccaatttttcggcggcggatttgggagcccatgtgatgaaaagcgtcctagcccaggccggggtggggggagaccagcttgacctgtacattatgggcaacgtgttaagggctggccatgggcaattgattccccggcaagcggccttgaaggcggagattcccgatacggtggacggttatgcagtggatatggtctgctcttccgccatgatgagtgtgattaatgcagctttaaccatccgggctggggagggggatttaattttagctggagggacggaatccatgtcccaaactggtttttacctttcccaccgggcccgctggggctacaagttcctcatgggagcaccggaaaatttaaccgatctcctgctccatgatggtttgacggacagcaccaatggggagggcatgggggaacagacggaaaaattggccgcagagcatggtttcagccggatagaattggacgaagtggcctgcttatcgcaacaaagggcggcccacgccacggaatctggttattttgactcagaaattgccccgattgaaatcaccagtcggaaaggaacccaggtgttagccagtgatgaaggtattcgcagtgacaccaccgtggaaagtctaggcaaattgcgctcggcctttgccaaggacggagtgttaacagcgggcaactgtagtcagattaccgatggggcggcagctttgctcctggccagtggggaagcggtggaaaaatatcaactcaaacccttggcaaaaattttgggaggcagttgggcggcgggtactcccagtcgttttcctgagttgcctattaccgctagccagaaactgttagcaaagctagataaaaccctggcagattttgacttgtttgaaaataatgaagccttctccgtcagcaatcttttatttgagcggcggttgggggtggatcgggacaagttgaatgtgaacggtggggcgatcgccctggggcatcccattggagcgtcgggggccaggatcatggttaccctactctatgccttgcaacaacgggataaaactctggggttagcagccctttgccatggcacggggggcggtacggcgatcgccttagagcgggtgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125502_sequence/1
type: Sequence
persistent identity: BBa K125502 sequence
display id: BBa_K125502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165006_sequence/1
type: Sequence
persistent identity: BBa K165006 sequence
display id: BBa_K165006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1021_sequence/1
type: Sequence
persistent identity: BBa M1021 sequence
display id: BBa_M1021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C2001_sequence/1
type: Sequence
persistent identity: BBa C2001 sequence
display id: BBa_C2001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1001_sequence/1
type: Sequence
persistent identity: BBa B1001 sequence
display id: BBa_B1001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcttcggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1020_sequence/1
type: Sequence
persistent identity: BBa M1020 sequence
display id: BBa_M1020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctggcaggtcaggcagg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z