LOCUS BBa_M1031 4796 bp DNA linear 23-Dec-2024 DEFINITION Total construct Pha, Phb, Phc plus zinc fingers and promoter ACCESSION BBa_M1031 VERSION BBa_M1031.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..57 /label=BBa_B0035 misc_feature 66..317 /label=BBa_K165006 misc_feature 1551..1553 /label=mt tGa to tAa misc_feature 1554..1556 /label=inserted second stop misc_feature 324..326 /label=start CDS 324..1556 /label=BBa_K125501 misc_feature 44..1556 /label=BBa_M1025 RBS 1565..1578 /label=BBa_B0035 CDS 1588..1674 /label=Finger 2 CDS 1675..1734 /label=Finger 3 misc_feature 1735..1749 /label=linker CDS 1750..1845 /label=GCN4: leucine zipper misc_feature 1846..1878 /label=LVA misc_feature 1879..1884 /label=2 misc_feature 1627..1647 /label=Recognition site misc_feature 1711..1731 /label=Recognition site misc_feature 1885..1909 /label=Help:Barcodes CDS 1585..1909 /label=BBa_C2001 misc_feature 1916..1918 /label=start misc_feature 2636..2638 /label=original stop codon misc_feature 2639..2641 /label=inserted second stop CDS 1916..2641 /label=BBa_K125502 CDS 1565..2641 /label=BBa_M1019 RBS 2650..2663 /label=BBa_B0035 CDS 2664..2978 /label=Blues Zinc Finger misc_feature 2664..2666 /label=start misc_feature 2979..2984 /label=Silver fusion Scar CDS 2985..4754 /label=phaC gene misc_feature 2985..2987 /label=start misc_feature 4752..4754 /label=stop CDS 2664..4754 /label=BBa_M1020 misc_feature 2650..4754 /label=BBa_M1021 stem_loop 4772..4787 /label=stem loop polyA_site 4788..4796 /label=Poly A tail polyA_site 4763..4771 /label=PolyA tail misc_feature 4763..4796 /label=B1001 terminator 4763..4796 /label=BBa_B1001 misc_feature 2650..4796 /label=BBa_M1023 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaatac 61 tagagccctt ccagtgtcgc atttgcatgc ggaacttttc gctcaggacg gaccttgaca 121 ggcatacccg tactcatacc ggtgaaaaac cgtttcagtg tcggatctgt atgcgaaatt 181 tctccctcag ccagaccttg cgccgccatc tacgtacgca caccggcgag aagccattcc 241 aatgccgaat atgcatgcgc aacttcagtc tcaggagcaa cctggggcgg cacctaaaaa 301 cccacacagg agaaaaatac tagatggccg cccatcccaa cccatggcat tggaaaacaa 361 ttatgcgtga tgtttttatt gttgccgcta aacgaactcc cctgggacgt tttggtggct 421 ccctgaccaa tttttcggcg gcggatttgg gagcccatgt gatgaaaagc gtcctagccc 481 aggccggggt ggggggagac cagcttgacc tgtacattat gggcaacgtg ttaagggctg 541 gccatgggca attgattccc cggcaagcgg ccttgaaggc ggagattccc gatacggtgg 601 acggttatgc agtggatatg gtctgctctt ccgccatgat gagtgtgatt aatgcagctt 661 taaccatccg ggctggggag ggggatttaa ttttagctgg agggacggaa tccatgtccc 721 aaactggttt ttacctttcc caccgggccc gctggggcta caagttcctc atgggagcac 781 cggaaaattt aaccgatctc ctgctccatg atggtttgac ggacagcacc aatggggagg 841 gcatggggga acagacggaa aaattggccg cagagcatgg tttcagccgg atagaattgg 901 acgaagtggc ctgcttatcg caacaaaggg cggcccacgc cacggaatct ggttattttg 961 actcagaaat tgccccgatt gaaatcacca gtcggaaagg aacccaggtg ttagccagtg 1021 atgaaggtat tcgcagtgac accaccgtgg aaagtctagg caaattgcgc tcggcctttg 1081 ccaaggacgg agtgttaaca gcgggcaact gtagtcagat taccgatggg gcggcagctt 1141 tgctcctggc cagtggggaa gcggtggaaa aatatcaact caaacccttg gcaaaaattt 1201 tgggaggcag ttgggcggcg ggtactccca gtcgttttcc tgagttgcct attaccgcta 1261 gccagaaact gttagcaaag ctagataaaa ccctggcaga ttttgacttg tttgaaaata 1321 atgaagcctt ctccgtcagc aatcttttat ttgagcggcg gttgggggtg gatcgggaca 1381 agttgaatgt gaacggtggg gcgatcgccc tggggcatcc cattggagcg tcgggggcca 1441 ggatcatggt taccctactc tatgccttgc aacaacggga taaaactctg gggttagcag 1501 ccctttgcca tggcacgggg ggcggtacgg cgatcgcctt agagcgggtg taataatact 1561 agagattaaa gaggagaata ctagatgaaa ccgttccagt gccgtatctg catgcgtaac 1621 ttctcccgtt ccgaccacct gaccacccac atccgtaccc acaccggtga aaaaccgttc 1681 gcttgcgaca tctgcggtcg taaattcgct cgttccgacg aacgtaaacg tcaccgtaaa 1741 ttgcagcaca tgaaacagct ggaagacaaa gttgaagaac tgctgtccaa aaactaccac 1801 ctggaaaacg aagttgctcg tctgaaaaaa ctggttggtg aacgtgctgc taacgacgaa 1861 aactacgctc tggttgctta ataactctga tagtgctagt gtagatctct actagatgtt 1921 aagtcttggt ttggaagata aagtaatcgt ggtcaccggc ggcaatcggg gcatcggcgc 1981 ggcgatcgtg aaattactcc aggaaatggg ggccaaagta gcttttaccg atttagctac 2041 ggacgggggt aatactgaag ccctgggggt ggtggccaac gtcaccgatt tggaatccat 2101 gacggcggcg gcagcggaaa tcaccgataa gctgggcccc gtttacggtg tggtggccaa 2161 tgccggtatc accaaagaca actttttccc aaaattaacc cccgccgatt gggatgcagt 2221 gttgaacgtt aacttgaaag gggtagccta cagcattaag cctttcatcg aaggcatgta 2281 tgaacggaaa gccggctcca ttgtggccat tagttccatc tccggggagc ggggtaacgt 2341 cggtcaaact aactattccg ccactaaagc tggggtaatt ggcatgatga aatccctggc 2401 tcgggaaggg gcccggtatg gagtgcgggc caatgcagta gcccctggtt tcattgacac 2461 cgaaatgact ttggcgatcc gggaagatat tcgggaaaaa attaccaagg aaatcccctt 2521 ccgccgtttt ggtaaaccgg aggaaattgc ctgggcggtg gcctttttac tttcccccgt 2581 agccagtagc tatgtcaccg gcgaagtatt acgggtaaat ggggcccacc acacctaata 2641 atactagaga ttaaagagga gaaatggcaa gcgatgatcg tccgtatgca tgtccggttg 2701 aaagctgtga tcgtcgtttt agccgtcgtg atgttctgat gaatcatatt cgtattcata 2761 ccggtcatga aaaaccgttt cagtgtcgta tttgtatgcg taattttagc cgtagcgatc 2821 atctgaccac ccatattcgt acccataccg gtgaaaaacc gtttgcatgt gatatttgtg 2881 gtcgtaaatt tgcaaatcgt gataccctga cccgtcatag caaaattcat ctgcgtcaga 2941 atgatctgga aggtggtagc ggtggtggta gcggtggtac tagaatggca accggtaaag 3001 gtgcagcagc aagcacccag gaaggtaaaa gccagccgtt taaagttacc ccgggtccgt 3061 ttgatccggc aacctggctg gaatggagcc gtcagtggca gggtaccgaa ggtaatggtc 3121 atgcagcagc aagcggtatt ccgggtctgg atgcactggc aggtgttaaa attgcaccgg 3181 cacagctggg tgatattcag cagcgttata tgaaagattt tagcgcactg tggcaggcaa 3241 tggcagaagg taaagcagaa gcaaccggtc cgctgcatga tcgtcgtttt gcaggtgatg 3301 catggcgtac caatctgccg tatcgttttg cagcagcatt ttatctgctg aatgcacgtg 3361 cactgaccga actggcagat gcagttgaag cagatgcaaa aacccgtcag cgtattcgtt 3421 ttgcaattag ccagtgggtt gatgcaatga gcccggcaaa ttttctggca accaatccgg 3481 aagcacagcg tctgctgatt gaaagcggtg gtgaaagcct gcgtgcaggt gttcgtaata 3541 tgatggaaga tctgacccgt ggtaaaatta gccagaccga tgaaagcgca tttgaagttg 3601 gtcgtaatgt tgcagttacc gaaggtgcag ttgtttttga aaatgaatat tttcagctgc 3661 tgcaatataa accgctgacc gataaagttc atgcacgtcc gctgctgatg gttccgccgt 3721 gtattaataa atattatatt ctggatctgc aaccggaaag cagcctggtt cgtcatgttg 3781 ttgaacaggg tcataccgtt tttctggtta gctggcgtaa tccggatgca agcatggcag 3841 gtagcacctg ggatgattat attgaacatg cagcaattcg tgcaattgaa gttgcacgtg 3901 atattagcgg tcaggataaa attaatgttc tgggtttttg tgttggtggt accattgtta 3961 gcaccgcact ggcagttctg gcagcacgtg gtgaacatcc ggcagcaagc gttaccctgc 4021 tgaccaccct gctggatttt gcagataccg gtattctgga tgtttttgtt gatgaaggtc 4081 atgttcagct gcgtgaagca accctgggtg gtggtgcagg tgcaccgtgt gcactgctgc 4141 gcggtctgga actggcaaat acctttagct ttctgcgtcc gaatgatctg gtttggaatt 4201 atgttgttga taattatctg aaaggtaata ccccggttcc gtttgatctg ctgttttgga 4261 atggtgatgc aaccaatctg ccgggtccgt ggtattgttg gtatctgcgt catacctatc 4321 tgcaaaatga actgaaagtt ccgggtaaac tgaccgtttg tggtgttccg gttgatctgg 4381 caagcattga tgttccgacc tatatttatg gtagccgtga agatcatatt gttccgtgga 4441 ccgcagcata tgcaagcacc gcactgctgg caaataaact gcgttttgtt ctgggtgcaa 4501 gcggtcatat tgcaggtgtt attaatccgc cggcaaaaaa taaacgtagc cattggacca 4561 atgatgcact gccggaaagc ccgcagcagt ggctggcagg tgcaattgaa catcatggta 4621 gctggtggcc ggattggacc gcatggctgg caggtcaggc aggtgcaaaa cgtgcagcac 4681 cggcaaatta tggtaatgca cgttatcgtg caattgaacc ggcaccgggt cgttatgtta 4741 aagcaaaagc ataatactag agaaaaaaaa accccgcttc ggcggggttt tttttt //