BBa_K125502 1 BBa_K125502 phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993) 2008-08-09T11:00:00Z 2015-05-08T01:09:45Z Synechocystis sp. PCC6803 phaB false false _179_ 0 2680 9 It's complicated false PHB biosynthesis pathway false Norman Wang annotation1971709 1 original stop codon range1971709 1 721 723 annotation1971710 1 inserted second stop range1971710 1 724 726 annotation1971708 1 start range1971708 1 1 3 BBa_M1031 1 BBa_M1031 Total construct Pha, Phb, Phc plus zinc fingers and promoter 2011-04-26T11:00:00Z 2015-05-08T01:13:52Z Parts registry This is the entire construct of the DNA to be inserted into the plasmid and put into E. Coli. All Pha, Phb, and Phc enzymes are silver fusion compatible to their respective Zinc fingers. false false _768_ 0 8955 9 Not in stock false This is silver fusion compatible false Neil Draper component2289160 1 BBa_M1019 component2289137 1 BBa_J23100 component2289144 1 BBa_M1025 component2289175 1 BBa_M1023 annotation2289137 1 BBa_J23100 range2289137 1 1 35 annotation2289144 1 BBa_M1025 range2289144 1 44 1556 annotation2289160 1 BBa_M1019 range2289160 1 1565 2641 annotation2289175 1 BBa_M1023 range2289175 1 2650 4796 BBa_C2001 1 zif23 Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli) 2004-01-29T12:00:00Z 2015-08-31T04:07:25Z Protein Engineering Gods from the Pabo Lab Released HQ 2013 Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added. false false _1_ 0 24 7 In stock false This protein should form a dimer and bind a specific operator site, thus repressing transcription. false Drew Endy annotation318104 1 linker range318104 1 151 165 annotation318109 1 Recognition site range318109 1 127 147 annotation318101 1 Finger 2 range318101 1 4 90 annotation2214011 1 Help:Barcodes range2214011 1 301 325 annotation318107 1 2 range318107 1 295 300 annotation318103 1 Finger 3 range318103 1 91 150 annotation318108 1 Recognition site range318108 1 43 63 annotation318106 1 LVA range318106 1 262 294 annotation318105 1 GCN4: leucine zipper range318105 1 166 261 BBa_K165006 1 BBa_K165006 Zif268-HIV DNA-binding domain 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated true - false John Szymanski BBa_M1019 1 BBa_M1019 Silver Fusion Compatible PhaB gene with zif23-GCN4 2011-04-18T11:00:00Z 2015-05-08T01:13:51Z N/A The bioplastic enzyme PhaB has been combined with a zinc finger to increase binding. Also this sequence is silver fusion compatible and has a scar in between the Zif23-GCN4 and the PhaB enzyme. The optimal RBS has been attached at the beginning of the sequence false false _768_ 0 8965 9 Not in stock false This sequence is supposed to be silver fusion compatible. false Michaela Hugie component2247704 1 BBa_K125502 component2247700 1 BBa_C2001 component2247690 1 BBa_B0035 annotation2247700 1 BBa_C2001 range2247700 1 21 345 annotation2247704 1 BBa_K125502 range2247704 1 352 1077 annotation2247690 1 BBa_B0035 range2247690 1 1 14 BBa_M1021 1 BBa_M1021 RBS+ "Blues" Zinc Finger + PhaC 2011-04-11T11:00:00Z 2015-05-08T01:13:52Z PhaC: A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. false false _768_ 0 8986 9 Not in stock false The coding sequences were fused using the Silver fusion standard. Both sequences are optimized for expression in E. coli false Miguel Suastegui component2117215 1 BBa_B0035 component2117222 1 BBa_M1020 annotation2117222 1 BBa_M1020 range2117222 1 15 2105 annotation2117215 1 BBa_B0035 range2117215 1 1 14 BBa_M1025 1 BBa_M1025 RBS and HIV Zn finger and PhaA pathway 2011-04-17T11:00:00Z 2015-05-08T01:13:52Z See existing parts BBa_K165006, BBa_K156012, and BBa_B0035 This composite part contains the ribosome binding site BBa_B0035 a binding domain and the coding region that encodes for the PhaA pathway. The binding domain that was used is the zif268 HIV zn finger. This domain binds to the sequence GAT GCT GCA. The last coding region that is in this composite part is the PhaA pathway that encodes 1/3 of the enzymes necessary to produce bioplastic. This part is silver fusion compatible. false false _768_ 0 8955 9 Not in stock false none false Neil Draper component2116131 1 BBa_K165006 component2116130 1 BBa_B0035 component2116135 1 BBa_K125501 annotation2116131 1 BBa_K165006 range2116131 1 23 274 annotation2116130 1 BBa_B0035 range2116130 1 1 14 annotation2116135 1 BBa_K125501 range2116135 1 281 1513 BBa_M1023 1 BBa_M1023 RBS+"Blues" Zinc Finger+ phaC+ terminator 2011-04-12T11:00:00Z 2015-05-08T01:13:52Z - "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. The designed part is compatible with the Silver fusion assembly standard. The codons were optimized for expression on E. coli. false false _768_ 0 8986 9 Not in stock false - false Miguel Suastegui component2117231 1 BBa_M1021 component2117236 1 BBa_B1001 annotation2117231 1 BBa_M1021 range2117231 1 1 2105 annotation2117236 1 BBa_B1001 range2117236 1 2114 2147 BBa_B0035 1 BBa_B0035 RBS (B0030 derivative) 2004-01-27T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 Not in stock false false Jason Kelly BBa_M1020 1 BBa_M1020 "Blues" Zinc Finger + PhaC 2011-04-11T11:00:00Z 2015-05-08T01:13:51Z PhaC: A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. false false _768_ 0 8986 9 Not in stock false The coding sequences were fused using the Silver fusion standard. Both sequences are optimized for expression in E. coli false Miguel Suastegui annotation2115852 1 phaC gene range2115852 1 322 2091 annotation2115851 1 Silver fusion Scar range2115851 1 316 321 annotation2115850 1 start range2115850 1 1 3 annotation2115849 1 Blues Zinc Finger range2115849 1 1 315 annotation2115854 1 stop range2115854 1 2089 2091 annotation2115853 1 start range2115853 1 322 324 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K125501 1 BBa_K125501 phaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994) 2008-08-09T11:00:00Z 2015-05-08T01:09:45Z Synechocystis sp. PCC6803 phaA false false _179_ 0 2680 9 It's complicated false PHB synthesis pathway false Norman Wang annotation1971707 1 start range1971707 1 1 3 annotation1971705 1 mt tGa to tAa range1971705 1 1228 1230 annotation1971706 1 inserted second stop range1971706 1 1231 1233 BBa_B1001 1 BBa_B1001 Terminator (artifical, small, %T~=90) 2006-08-29T11:00:00Z 2015-08-31T04:07:21Z antiquity Artificial terminator, estimated %T>90 false false _41_ 0 745 41 In stock false Poly T tail of 9 residues for high %T true Haiyao Huang annotation1898379 1 stem loop range1898379 1 10 25 annotation1898382 1 B1001 range1898382 1 1 34 annotation1898381 1 PolyA tail range1898381 1 1 9 annotation1898380 1 Poly A tail range1898380 1 26 34 BBa_M1020_sequence 1 atggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctggcaggtcaggcaggtgcaaaacgtgcagcaccggcaaattatggtaatgcacgttatcgtgcaattgaaccggcaccgggtcgttatgttaaagcaaaagcataa BBa_K125502_sequence 1 atgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataa BBa_B0035_sequence 1 attaaagaggagaa BBa_M1023_sequence 1 attaaagaggagaaatggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctggcaggtcaggcaggtgcaaaacgtgcagcaccggcaaattatggtaatgcacgttatcgtgcaattgaaccggcaccgggtcgttatgttaaagcaaaagcataatactagagaaaaaaaaaccccgcttcggcggggttttttttt BBa_M1021_sequence 1 attaaagaggagaaatggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctggcaggtcaggcaggtgcaaaacgtgcagcaccggcaaattatggtaatgcacgttatcgtgcaattgaaccggcaccgggtcgttatgttaaagcaaaagcataa BBa_K125501_sequence 1 atggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaactcccctgggacgttttggtggctccctgaccaatttttcggcggcggatttgggagcccatgtgatgaaaagcgtcctagcccaggccggggtggggggagaccagcttgacctgtacattatgggcaacgtgttaagggctggccatgggcaattgattccccggcaagcggccttgaaggcggagattcccgatacggtggacggttatgcagtggatatggtctgctcttccgccatgatgagtgtgattaatgcagctttaaccatccgggctggggagggggatttaattttagctggagggacggaatccatgtcccaaactggtttttacctttcccaccgggcccgctggggctacaagttcctcatgggagcaccggaaaatttaaccgatctcctgctccatgatggtttgacggacagcaccaatggggagggcatgggggaacagacggaaaaattggccgcagagcatggtttcagccggatagaattggacgaagtggcctgcttatcgcaacaaagggcggcccacgccacggaatctggttattttgactcagaaattgccccgattgaaatcaccagtcggaaaggaacccaggtgttagccagtgatgaaggtattcgcagtgacaccaccgtggaaagtctaggcaaattgcgctcggcctttgccaaggacggagtgttaacagcgggcaactgtagtcagattaccgatggggcggcagctttgctcctggccagtggggaagcggtggaaaaatatcaactcaaacccttggcaaaaattttgggaggcagttgggcggcgggtactcccagtcgttttcctgagttgcctattaccgctagccagaaactgttagcaaagctagataaaaccctggcagattttgacttgtttgaaaataatgaagccttctccgtcagcaatcttttatttgagcggcggttgggggtggatcgggacaagttgaatgtgaacggtggggcgatcgccctggggcatcccattggagcgtcgggggccaggatcatggttaccctactctatgccttgcaacaacgggataaaactctggggttagcagccctttgccatggcacggggggcggtacggcgatcgccttagagcgggtgtaataa BBa_M1031_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagatggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaactcccctgggacgttttggtggctccctgaccaatttttcggcggcggatttgggagcccatgtgatgaaaagcgtcctagcccaggccggggtggggggagaccagcttgacctgtacattatgggcaacgtgttaagggctggccatgggcaattgattccccggcaagcggccttgaaggcggagattcccgatacggtggacggttatgcagtggatatggtctgctcttccgccatgatgagtgtgattaatgcagctttaaccatccgggctggggagggggatttaattttagctggagggacggaatccatgtcccaaactggtttttacctttcccaccgggcccgctggggctacaagttcctcatgggagcaccggaaaatttaaccgatctcctgctccatgatggtttgacggacagcaccaatggggagggcatgggggaacagacggaaaaattggccgcagagcatggtttcagccggatagaattggacgaagtggcctgcttatcgcaacaaagggcggcccacgccacggaatctggttattttgactcagaaattgccccgattgaaatcaccagtcggaaaggaacccaggtgttagccagtgatgaaggtattcgcagtgacaccaccgtggaaagtctaggcaaattgcgctcggcctttgccaaggacggagtgttaacagcgggcaactgtagtcagattaccgatggggcggcagctttgctcctggccagtggggaagcggtggaaaaatatcaactcaaacccttggcaaaaattttgggaggcagttgggcggcgggtactcccagtcgttttcctgagttgcctattaccgctagccagaaactgttagcaaagctagataaaaccctggcagattttgacttgtttgaaaataatgaagccttctccgtcagcaatcttttatttgagcggcggttgggggtggatcgggacaagttgaatgtgaacggtggggcgatcgccctggggcatcccattggagcgtcgggggccaggatcatggttaccctactctatgccttgcaacaacgggataaaactctggggttagcagccctttgccatggcacggggggcggtacggcgatcgccttagagcgggtgtaataatactagagattaaagaggagaatactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataatactagagattaaagaggagaaatggcaagcgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtcgtgatgttctgatgaatcatattcgtattcataccggtcatgaaaaaccgtttcagtgtcgtatttgtatgcgtaattttagccgtagcgatcatctgaccacccatattcgtacccataccggtgaaaaaccgtttgcatgtgatatttgtggtcgtaaatttgcaaatcgtgataccctgacccgtcatagcaaaattcatctgcgtcagaatgatctggaaggtggtagcggtggtggtagcggtggtactagaatggcaaccggtaaaggtgcagcagcaagcacccaggaaggtaaaagccagccgtttaaagttaccccgggtccgtttgatccggcaacctggctggaatggagccgtcagtggcagggtaccgaaggtaatggtcatgcagcagcaagcggtattccgggtctggatgcactggcaggtgttaaaattgcaccggcacagctgggtgatattcagcagcgttatatgaaagattttagcgcactgtggcaggcaatggcagaaggtaaagcagaagcaaccggtccgctgcatgatcgtcgttttgcaggtgatgcatggcgtaccaatctgccgtatcgttttgcagcagcattttatctgctgaatgcacgtgcactgaccgaactggcagatgcagttgaagcagatgcaaaaacccgtcagcgtattcgttttgcaattagccagtgggttgatgcaatgagcccggcaaattttctggcaaccaatccggaagcacagcgtctgctgattgaaagcggtggtgaaagcctgcgtgcaggtgttcgtaatatgatggaagatctgacccgtggtaaaattagccagaccgatgaaagcgcatttgaagttggtcgtaatgttgcagttaccgaaggtgcagttgtttttgaaaatgaatattttcagctgctgcaatataaaccgctgaccgataaagttcatgcacgtccgctgctgatggttccgccgtgtattaataaatattatattctggatctgcaaccggaaagcagcctggttcgtcatgttgttgaacagggtcataccgtttttctggttagctggcgtaatccggatgcaagcatggcaggtagcacctgggatgattatattgaacatgcagcaattcgtgcaattgaagttgcacgtgatattagcggtcaggataaaattaatgttctgggtttttgtgttggtggtaccattgttagcaccgcactggcagttctggcagcacgtggtgaacatccggcagcaagcgttaccctgctgaccaccctgctggattttgcagataccggtattctggatgtttttgttgatgaaggtcatgttcagctgcgtgaagcaaccctgggtggtggtgcaggtgcaccgtgtgcactgctgcgcggtctggaactggcaaatacctttagctttctgcgtccgaatgatctggtttggaattatgttgttgataattatctgaaaggtaataccccggttccgtttgatctgctgttttggaatggtgatgcaaccaatctgccgggtccgtggtattgttggtatctgcgtcatacctatctgcaaaatgaactgaaagttccgggtaaactgaccgtttgtggtgttccggttgatctggcaagcattgatgttccgacctatatttatggtagccgtgaagatcatattgttccgtggaccgcagcatatgcaagcaccgcactgctggcaaataaactgcgttttgttctgggtgcaagcggtcatattgcaggtgttattaatccgccggcaaaaaataaacgtagccattggaccaatgatgcactgccggaaagcccgcagcagtggctggcaggtgcaattgaacatcatggtagctggtggccggattggaccgcatggctggcaggtcaggcaggtgcaaaacgtgcagcaccggcaaattatggtaatgcacgttatcgtgcaattgaaccggcaccgggtcgttatgttaaagcaaaagcataatactagagaaaaaaaaaccccgcttcggcggggttttttttt BBa_M1025_sequence 1 attaaagaggagaatactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagatggccgcccatcccaacccatggcattggaaaacaattatgcgtgatgtttttattgttgccgctaaacgaactcccctgggacgttttggtggctccctgaccaatttttcggcggcggatttgggagcccatgtgatgaaaagcgtcctagcccaggccggggtggggggagaccagcttgacctgtacattatgggcaacgtgttaagggctggccatgggcaattgattccccggcaagcggccttgaaggcggagattcccgatacggtggacggttatgcagtggatatggtctgctcttccgccatgatgagtgtgattaatgcagctttaaccatccgggctggggagggggatttaattttagctggagggacggaatccatgtcccaaactggtttttacctttcccaccgggcccgctggggctacaagttcctcatgggagcaccggaaaatttaaccgatctcctgctccatgatggtttgacggacagcaccaatggggagggcatgggggaacagacggaaaaattggccgcagagcatggtttcagccggatagaattggacgaagtggcctgcttatcgcaacaaagggcggcccacgccacggaatctggttattttgactcagaaattgccccgattgaaatcaccagtcggaaaggaacccaggtgttagccagtgatgaaggtattcgcagtgacaccaccgtggaaagtctaggcaaattgcgctcggcctttgccaaggacggagtgttaacagcgggcaactgtagtcagattaccgatggggcggcagctttgctcctggccagtggggaagcggtggaaaaatatcaactcaaacccttggcaaaaattttgggaggcagttgggcggcgggtactcccagtcgttttcctgagttgcctattaccgctagccagaaactgttagcaaagctagataaaaccctggcagattttgacttgtttgaaaataatgaagccttctccgtcagcaatcttttatttgagcggcggttgggggtggatcgggacaagttgaatgtgaacggtggggcgatcgccctggggcatcccattggagcgtcgggggccaggatcatggttaccctactctatgccttgcaacaacgggataaaactctggggttagcagccctttgccatggcacggggggcggtacggcgatcgccttagagcgggtgtaataa BBa_C2001_sequence 1 atgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K165006_sequence 1 cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaa BBa_B1001_sequence 1 aaaaaaaaaccccgcttcggcggggttttttttt BBa_M1019_sequence 1 attaaagaggagaatactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgtgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagatgttaagtcttggtttggaagataaagtaatcgtggtcaccggcggcaatcggggcatcggcgcggcgatcgtgaaattactccaggaaatgggggccaaagtagcttttaccgatttagctacggacgggggtaatactgaagccctgggggtggtggccaacgtcaccgatttggaatccatgacggcggcggcagcggaaatcaccgataagctgggccccgtttacggtgtggtggccaatgccggtatcaccaaagacaactttttcccaaaattaacccccgccgattgggatgcagtgttgaacgttaacttgaaaggggtagcctacagcattaagcctttcatcgaaggcatgtatgaacggaaagccggctccattgtggccattagttccatctccggggagcggggtaacgtcggtcaaactaactattccgccactaaagctggggtaattggcatgatgaaatccctggctcgggaaggggcccggtatggagtgcgggccaatgcagtagcccctggtttcattgacaccgaaatgactttggcgatccgggaagatattcgggaaaaaattaccaaggaaatccccttccgccgttttggtaaaccggaggaaattgcctgggcggtggcctttttactttcccccgtagccagtagctatgtcaccggcgaagtattacgggtaaatggggcccaccacacctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z