LOCUS BBa_M1032 4753 bp DNA linear 23-Dec-2024 DEFINITION PhaA PhaB PhaC and zinc fingers ACCESSION BBa_M1032 VERSION BBa_M1032.1 FEATURES Location/Qualifiers RBS 1..14 /label=BBa_B0035 misc_feature 23..274 /label=BBa_K165006 misc_feature 1508..1510 /label=mt tGa to tAa misc_feature 1511..1513 /label=inserted second stop misc_feature 281..283 /label=start CDS 281..1513 /label=BBa_K125501 misc_feature 1..1513 /label=BBa_M1025 RBS 1522..1535 /label=BBa_B0035 CDS 1545..1631 /label=Finger 2 CDS 1632..1691 /label=Finger 3 misc_feature 1692..1706 /label=linker CDS 1707..1802 /label=GCN4: leucine zipper misc_feature 1803..1835 /label=LVA misc_feature 1836..1841 /label=2 misc_feature 1584..1604 /label=Recognition site misc_feature 1668..1688 /label=Recognition site misc_feature 1842..1866 /label=Help:Barcodes CDS 1542..1866 /label=BBa_C2001 misc_feature 1873..1875 /label=start misc_feature 2593..2595 /label=original stop codon misc_feature 2596..2598 /label=inserted second stop CDS 1873..2598 /label=BBa_K125502 CDS 1522..2598 /label=BBa_M1019 RBS 2607..2620 /label=BBa_B0035 CDS 2621..2935 /label=Blues Zinc Finger misc_feature 2621..2623 /label=start misc_feature 2936..2941 /label=Silver fusion Scar CDS 2942..4711 /label=phaC gene misc_feature 2942..2944 /label=start misc_feature 4709..4711 /label=stop CDS 2621..4711 /label=BBa_M1020 misc_feature 2607..4711 /label=BBa_M1021 stem_loop 4729..4744 /label=stem loop polyA_site 4745..4753 /label=Poly A tail polyA_site 4720..4728 /label=PolyA tail misc_feature 4720..4753 /label=B1001 terminator 4720..4753 /label=BBa_B1001 misc_feature 2607..4753 /label=BBa_M1023 ORIGIN 1 attaaagagg agaatactag agcccttcca gtgtcgcatt tgcatgcgga acttttcgct 61 caggacggac cttgacaggc atacccgtac tcataccggt gaaaaaccgt ttcagtgtcg 121 gatctgtatg cgaaatttct ccctcagcca gaccttgcgc cgccatctac gtacgcacac 181 cggcgagaag ccattccaat gccgaatatg catgcgcaac ttcagtctca ggagcaacct 241 ggggcggcac ctaaaaaccc acacaggaga aaaatactag atggccgccc atcccaaccc 301 atggcattgg aaaacaatta tgcgtgatgt ttttattgtt gccgctaaac gaactcccct 361 gggacgtttt ggtggctccc tgaccaattt ttcggcggcg gatttgggag cccatgtgat 421 gaaaagcgtc ctagcccagg ccggggtggg gggagaccag cttgacctgt acattatggg 481 caacgtgtta agggctggcc atgggcaatt gattccccgg caagcggcct tgaaggcgga 541 gattcccgat acggtggacg gttatgcagt ggatatggtc tgctcttccg ccatgatgag 601 tgtgattaat gcagctttaa ccatccgggc tggggagggg gatttaattt tagctggagg 661 gacggaatcc atgtcccaaa ctggttttta cctttcccac cgggcccgct ggggctacaa 721 gttcctcatg ggagcaccgg aaaatttaac cgatctcctg ctccatgatg gtttgacgga 781 cagcaccaat ggggagggca tgggggaaca gacggaaaaa ttggccgcag agcatggttt 841 cagccggata gaattggacg aagtggcctg cttatcgcaa caaagggcgg cccacgccac 901 ggaatctggt tattttgact cagaaattgc cccgattgaa atcaccagtc ggaaaggaac 961 ccaggtgtta gccagtgatg aaggtattcg cagtgacacc accgtggaaa gtctaggcaa 1021 attgcgctcg gcctttgcca aggacggagt gttaacagcg ggcaactgta gtcagattac 1081 cgatggggcg gcagctttgc tcctggccag tggggaagcg gtggaaaaat atcaactcaa 1141 acccttggca aaaattttgg gaggcagttg ggcggcgggt actcccagtc gttttcctga 1201 gttgcctatt accgctagcc agaaactgtt agcaaagcta gataaaaccc tggcagattt 1261 tgacttgttt gaaaataatg aagccttctc cgtcagcaat cttttatttg agcggcggtt 1321 gggggtggat cgggacaagt tgaatgtgaa cggtggggcg atcgccctgg ggcatcccat 1381 tggagcgtcg ggggccagga tcatggttac cctactctat gccttgcaac aacgggataa 1441 aactctgggg ttagcagccc tttgccatgg cacggggggc ggtacggcga tcgccttaga 1501 gcgggtgtaa taatactaga gattaaagag gagaatacta gatgaaaccg ttccagtgcc 1561 gtatctgcat gcgtaacttc tcccgttccg accacctgac cacccacatc cgtacccaca 1621 ccggtgaaaa accgttcgct tgcgacatct gcggtcgtaa attcgctcgt tccgacgaac 1681 gtaaacgtca ccgtaaattg cagcacatga aacagctgga agacaaagtt gaagaactgc 1741 tgtccaaaaa ctaccacctg gaaaacgaag ttgctcgtct gaaaaaactg gttggtgaac 1801 gtgctgctaa cgacgaaaac tacgctctgg ttgcttaata actctgatag tgctagtgta 1861 gatctctact agatgttaag tcttggtttg gaagataaag taatcgtggt caccggcggc 1921 aatcggggca tcggcgcggc gatcgtgaaa ttactccagg aaatgggggc caaagtagct 1981 tttaccgatt tagctacgga cgggggtaat actgaagccc tgggggtggt ggccaacgtc 2041 accgatttgg aatccatgac ggcggcggca gcggaaatca ccgataagct gggccccgtt 2101 tacggtgtgg tggccaatgc cggtatcacc aaagacaact ttttcccaaa attaaccccc 2161 gccgattggg atgcagtgtt gaacgttaac ttgaaagggg tagcctacag cattaagcct 2221 ttcatcgaag gcatgtatga acggaaagcc ggctccattg tggccattag ttccatctcc 2281 ggggagcggg gtaacgtcgg tcaaactaac tattccgcca ctaaagctgg ggtaattggc 2341 atgatgaaat ccctggctcg ggaaggggcc cggtatggag tgcgggccaa tgcagtagcc 2401 cctggtttca ttgacaccga aatgactttg gcgatccggg aagatattcg ggaaaaaatt 2461 accaaggaaa tccccttccg ccgttttggt aaaccggagg aaattgcctg ggcggtggcc 2521 tttttacttt cccccgtagc cagtagctat gtcaccggcg aagtattacg ggtaaatggg 2581 gcccaccaca cctaataata ctagagatta aagaggagaa atggcaagcg atgatcgtcc 2641 gtatgcatgt ccggttgaaa gctgtgatcg tcgttttagc cgtcgtgatg ttctgatgaa 2701 tcatattcgt attcataccg gtcatgaaaa accgtttcag tgtcgtattt gtatgcgtaa 2761 ttttagccgt agcgatcatc tgaccaccca tattcgtacc cataccggtg aaaaaccgtt 2821 tgcatgtgat atttgtggtc gtaaatttgc aaatcgtgat accctgaccc gtcatagcaa 2881 aattcatctg cgtcagaatg atctggaagg tggtagcggt ggtggtagcg gtggtactag 2941 aatggcaacc ggtaaaggtg cagcagcaag cacccaggaa ggtaaaagcc agccgtttaa 3001 agttaccccg ggtccgtttg atccggcaac ctggctggaa tggagccgtc agtggcaggg 3061 taccgaaggt aatggtcatg cagcagcaag cggtattccg ggtctggatg cactggcagg 3121 tgttaaaatt gcaccggcac agctgggtga tattcagcag cgttatatga aagattttag 3181 cgcactgtgg caggcaatgg cagaaggtaa agcagaagca accggtccgc tgcatgatcg 3241 tcgttttgca ggtgatgcat ggcgtaccaa tctgccgtat cgttttgcag cagcatttta 3301 tctgctgaat gcacgtgcac tgaccgaact ggcagatgca gttgaagcag atgcaaaaac 3361 ccgtcagcgt attcgttttg caattagcca gtgggttgat gcaatgagcc cggcaaattt 3421 tctggcaacc aatccggaag cacagcgtct gctgattgaa agcggtggtg aaagcctgcg 3481 tgcaggtgtt cgtaatatga tggaagatct gacccgtggt aaaattagcc agaccgatga 3541 aagcgcattt gaagttggtc gtaatgttgc agttaccgaa ggtgcagttg tttttgaaaa 3601 tgaatatttt cagctgctgc aatataaacc gctgaccgat aaagttcatg cacgtccgct 3661 gctgatggtt ccgccgtgta ttaataaata ttatattctg gatctgcaac cggaaagcag 3721 cctggttcgt catgttgttg aacagggtca taccgttttt ctggttagct ggcgtaatcc 3781 ggatgcaagc atggcaggta gcacctggga tgattatatt gaacatgcag caattcgtgc 3841 aattgaagtt gcacgtgata ttagcggtca ggataaaatt aatgttctgg gtttttgtgt 3901 tggtggtacc attgttagca ccgcactggc agttctggca gcacgtggtg aacatccggc 3961 agcaagcgtt accctgctga ccaccctgct ggattttgca gataccggta ttctggatgt 4021 ttttgttgat gaaggtcatg ttcagctgcg tgaagcaacc ctgggtggtg gtgcaggtgc 4081 accgtgtgca ctgctgcgcg gtctggaact ggcaaatacc tttagctttc tgcgtccgaa 4141 tgatctggtt tggaattatg ttgttgataa ttatctgaaa ggtaataccc cggttccgtt 4201 tgatctgctg ttttggaatg gtgatgcaac caatctgccg ggtccgtggt attgttggta 4261 tctgcgtcat acctatctgc aaaatgaact gaaagttccg ggtaaactga ccgtttgtgg 4321 tgttccggtt gatctggcaa gcattgatgt tccgacctat atttatggta gccgtgaaga 4381 tcatattgtt ccgtggaccg cagcatatgc aagcaccgca ctgctggcaa ataaactgcg 4441 ttttgttctg ggtgcaagcg gtcatattgc aggtgttatt aatccgccgg caaaaaataa 4501 acgtagccat tggaccaatg atgcactgcc ggaaagcccg cagcagtggc tggcaggtgc 4561 aattgaacat catggtagct ggtggccgga ttggaccgca tggctggcag gtcaggcagg 4621 tgcaaaacgt gcagcaccgg caaattatgg taatgcacgt tatcgtgcaa ttgaaccggc 4681 accgggtcgt tatgttaaag caaaagcata atactagaga aaaaaaaacc ccgcttcggc 4741 ggggtttttt ttt //