LOCUS BBa_M11079 2608 bp DNA linear 23-Dec-2024 DEFINITION Copper binding paired with Polystyrene binding ACCESSION BBa_M11079 VERSION BBa_M11079.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..68 /label=RBS RBS 63..73 /label=BBa_I11010 misc_feature 80..82 /label=Start codon CDS 80..148 /label=Native Starting Signal misc_feature 149..163 /label=SfiI Restriction Site CDS 170..208 /label=Polystyrene Sticky Peptide misc_feature 254..271 /label=Linker CDS 272..556 /label=S54-F148 of OmpX misc_feature 557..568 /label=Linker CDS 569..727 /label=A1-S53 of OmpX misc_feature 728..733 /label=Double stop codon (TGATAA) misc_feature 164..169 /label=BglII Cut Site misc_feature 221..253 /label=T7 Antibody Tag misc_feature 209..214 /label=SalI Restriction Site CDS 215..220 /label=Trypsin Cleavage Site CDS 80..733 /label=Coding sequence CDS 80..733 /label=BBa_I728500 stem_loop 753..796 /label=stem_loop misc_feature 742..821 /label=BBa_B0010 terminator 742..821 /label=BBa_B0010 stem_loop 837..856 /label=T7 TE misc_feature 863..863 /label=stop polyA_site 857..870 /label=polya misc_feature 830..870 /label=BBa_B0012 terminator 830..870 /label=BBa_B0012 misc_feature 879..897 /label=TetR 1 misc_feature 879..932 /label=BBa_R0040 promoter 898..903 /label=-35 misc_feature 904..922 /label=TetR 2 promoter 921..926 /label=-10 promoter 879..932 /label=BBa_R0040 RBS 941..946 /label=RBS RBS 941..951 /label=BBa_I11010 misc_feature 1926..2264 /label=Copper binding Protein misc_feature 2265..2265 /label=Filler misc_feature 1925..1925 /label=Filler misc_feature 958..960 /label=start misc_feature 2469..2471 /label=stop CDS 958..2471 /label=BBa_M11078 stem_loop 2491..2534 /label=stem_loop misc_feature 2480..2559 /label=BBa_B0010 terminator 2480..2559 /label=BBa_B0010 stem_loop 2575..2594 /label=T7 TE misc_feature 2601..2601 /label=stop polyA_site 2595..2608 /label=polya misc_feature 2568..2608 /label=BBa_B0012 terminator 2568..2608 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaggaggtc atctactaga tgaaaaaaat tgcgtgcctg agcgcgctgg ccgcggttct 121 ggcctttacc gcgggcacca gcgttgcggg ccagtctggc caaagatctt ttttcagctt 181 tttttttccg gcgagcgcgt ggggtagcgt cgacaaacgt atggcgagca tgaccggcgg 241 tcagcagatg ggtggtggcc agagcggcca gagcggtgat tataacaaaa accagtatta 301 tggcattacc gcgggtccgg cgtatcgtat taacgattgg gcgagcattt atggcgtggt 361 gggcgtgggc tatggcaaat ttcagaccac cgaatatccg acctataaac acgataccag 421 cgattatggc tttagctatg gcgcgggtct gcaatttaat ccgatggaaa acgtggcgct 481 ggattttagc tatgaacaga gccgtattcg tagcgtggat gtgggcacct ggattgcggg 541 cgtgggttat cgttttggcg gcagcggtgc gaccagcacc gtgaccggcg gttatgcgca 601 gagcgatgcg cagggccaga tgaacaaaat gggcggcttc aacctgaaat atcgctatga 661 agaagataac agcccgctgg gcgtgattgg cagctttacc tataccgaaa aaagccgtac 721 cgcgagctga taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 781 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 841 caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg 901 acatccctat cagtgataga gatactgagc actactagag aggaggtcat ctactagatg 961 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct 1021 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg 1081 cactatttct ctgacaacaa agatgtagat ggcgaccaga cctacatgcg tcttggcttc 1141 aaaggtgaaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc 1201 cagggcaaca gcgctgaaaa cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg 1261 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttatgacgta 1321 acttcctgga ccgacgtact gccagaattc ggtggtgaca cctacggttc tgacaacttc 1381 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt 1441 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gcaacccatc tggtgaaggc 1501 tttactagtg gcgtaactaa caacggtcgt gacgcactgc gtcaaaacgg cgacggcgtc 1561 ggcggttcta tcacttatga ttacgaaggt ttcggtatcg gtggtgcgat ctccagctcc 1621 aaacgtactg atgctcagaa caccgctgct tacatcggta acggcgaccg tgctgaaacc 1681 tacactggtg gtctgaaata cgacgctaac aacatctacc tggctgctca gtacacccag 1741 acctacaacg caactcgcgt aggttccctg ggttgggcga acaaagcaca gaacttcgaa 1801 gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt ccctggctta cctgccaaca 1861 tcaacatatt tcaggatatc ttcgtcgtcg tagccacgac ccaggttttt acctttagac 1921 tgcaaatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtacggcac 1981 cagatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct 2041 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag 2101 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat 2161 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg 2221 gagacacaga ttacctctca tggctcaacg catctttacg ctgacagtct aaaggtaaaa 2281 acctgggtcg tggctacgac gacgaagata tcctgaaata tgttgatgtt ggtgctacct 2341 actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg ctggacgaca 2401 accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg ggtctggttt 2461 accagttcta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2521 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2581 ccttcgggtg ggcctttctg cgtttata //