##gff-version 3
##sequence-region BBa_M11079 1 2608
BBa_M11079	.	promoter	879	932	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_M11079	.	promoter	921	926	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M11079	.	promoter	898	903	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M11079	.	non_covalent_binding_site	904	922	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M11079	.	non_covalent_binding_site	879	897	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M11079	.	engineered_region	879	932	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M11079	.	CDS	80	733	.	+	0	ID=BBa_I728500;Name=BBa_I728500
BBa_M11079	.	sequence_feature	164	169	.	+	0	ID=annotation1940509;Name=BglII Cut Site;Parent=BBa_I728500
BBa_M11079	.	CDS	80	733	.	+	0	ID=annotation1975607;Name=Coding sequence;Parent=BBa_I728500
BBa_M11079	.	CDS	170	208	.	+	0	ID=annotation1940503;Name=Polystyrene Sticky Peptide;Parent=BBa_I728500
BBa_M11079	.	CDS	272	556	.	+	0	ID=annotation1940505;Name=S54-F148 of OmpX;Parent=BBa_I728500
BBa_M11079	.	CDS	215	220	.	+	0	ID=annotation1940512;Name=Trypsin Cleavage Site;Parent=BBa_I728500
BBa_M11079	.	sequence_feature	254	271	.	+	0	ID=annotation1940504;Name=Linker;Parent=BBa_I728500
BBa_M11079	.	sequence_feature	149	163	.	+	0	ID=annotation1940502;Name=SfiI Restriction Site;Parent=BBa_I728500
BBa_M11079	.	CDS	569	727	.	+	0	ID=annotation1940507;Name=A1-S53 of OmpX;Parent=BBa_I728500
BBa_M11079	.	CDS	80	148	.	+	0	ID=annotation1940501;Name=Native Starting Signal;Parent=BBa_I728500
BBa_M11079	.	stop_codon	728	733	.	+	0	ID=annotation1940508;Name=Double stop codon (TGATAA);Parent=BBa_I728500
BBa_M11079	.	start_codon	80	82	.	+	0	ID=annotation1940500;Name=Start codon;Parent=BBa_I728500
BBa_M11079	.	tag	221	253	.	+	0	ID=annotation1940510;Name=T7 Antibody Tag;Parent=BBa_I728500
BBa_M11079	.	sequence_feature	209	214	.	+	0	ID=annotation1940511;Name=SalI Restriction Site;Parent=BBa_I728500
BBa_M11079	.	sequence_feature	557	568	.	+	0	ID=annotation1940506;Name=Linker;Parent=BBa_I728500
BBa_M11079	.	terminator	2568	2608	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M11079	.	stop_codon	2601	2601	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M11079	.	stem_loop	2575	2594	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M11079	.	engineered_region	2568	2608	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M11079	.	polyA_site	2595	2608	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M11079	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_M11079	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M11079	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M11079	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M11079	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M11079	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M11079	.	ribosome_entry_site	63	73	.	+	0	ID=BBa_I11010;Name=BBa_I11010
BBa_M11079	.	ribosome_entry_site	63	68	.	+	0	ID=annotation1147662;Name=RBS;Parent=BBa_I11010
BBa_M11079	.	terminator	830	870	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M11079	.	stop_codon	863	863	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M11079	.	stem_loop	837	856	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M11079	.	engineered_region	830	870	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M11079	.	polyA_site	857	870	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M11079	.	CDS	958	2471	.	+	0	ID=BBa_M11078;Name=BBa_M11078
BBa_M11079	.	stop_codon	2469	2471	.	+	0	ID=annotation2002431;Name=stop;Parent=BBa_M11078
BBa_M11079	.	sequence_feature	1925	1925	.	+	0	ID=annotation2002426;Name=Filler;Parent=BBa_M11078
BBa_M11079	.	sequence_feature	1926	2264	.	+	0	ID=annotation2002395;Name=Copper binding Protein;Parent=BBa_M11078
BBa_M11079	.	sequence_feature	2265	2265	.	+	0	ID=annotation2002425;Name=Filler;Parent=BBa_M11078
BBa_M11079	.	start_codon	958	960	.	+	0	ID=annotation2002430;Name=start;Parent=BBa_M11078
BBa_M11079	.	terminator	742	821	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M11079	.	stem_loop	753	796	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M11079	.	engineered_region	742	821	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M11079	.	terminator	2480	2559	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M11079	.	stem_loop	2491	2534	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M11079	.	engineered_region	2480	2559	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M11079	.	ribosome_entry_site	941	951	.	+	0	ID=BBa_I11010;Name=BBa_I11010
BBa_M11079	.	ribosome_entry_site	941	946	.	+	0	ID=annotation1147662;Name=RBS;Parent=BBa_I11010
>BBa_M11079
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtcatctactag
atgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctg
gccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccgg
cggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggt
ccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaat
atccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgt
ggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgt
tttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgg
gcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaa
aagccgtaccgcgagctgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtc
atctactagatgaaagttaaagtactgtccctcctggtcccagctctgctggtagcaggcgcagcaaacgctgctgaag
tttacaacaaagacggcaacaaattagatctgtacggtaaagtagacggcctgcactatttctctgacaacaaagatgt
agatggcgaccagacctacatgcgtcttggcttcaaaggtgaaactcaggttactgaccagctgaccggttacggccag
tgggaatatcagatccagggcaacagcgctgaaaacgaaaacaactcctggacccgtgtggcattcgcaggtctgaaat
tccaggatgtgggttctttcgactacggtcgtaactacggcgttgtttatgacgtaacttcctggaccgacgtactgcc
agaattcggtggtgacacctacggttctgacaacttcatgcagcagcgtggtaacggcttcgcgacctaccgtaacact
gacttcttcggtctggttgacggcctgaactttgctgttcagtaccagggtaaaaacggcaacccatctggtgaaggct
ttactagtggcgtaactaacaacggtcgtgacgcactgcgtcaaaacggcgacggcgtcggcggttctatcacttatga
ttacgaaggtttcggtatcggtggtgcgatctccagctccaaacgtactgatgctcagaacaccgctgcttacatcggt
aacggcgaccgtgctgaaacctacactggtggtctgaaatacgacgctaacaacatctacctggctgctcagtacaccc
agacctacaacgcaactcgcgtaggttccctgggttgggcgaacaaagcacagaacttcgaagctgttgctcagtacca
gttcgacttcggtctgcgtccgtccctggcttacctgccaacatcaacatatttcaggatatcttcgtcgtcgtagcca
cgacccaggtttttacctttagactgcaaatgcttgatgaaaaaagttcgaataccgcgtctgtcgtggtgctatgtac
ggcaccagatgaagcgacagcccaggatttagccgccaaagtgctggcggaaaaactggcggcctgcgcgaccttgatc
cccggcgctacctctctctattactgggaaggtaagctggagcaagaatacgaagtgcagatgattttaaaaactaccg
tatctcaccagcaggcactgctggaatgcctgaagtctcatcatccatatcaaaccccggaacttctggttttacctgt
tacacacggagacacagattacctctcatggctcaacgcatctttacgctgacagtctaaaggtaaaaacctgggtcgt
ggctacgacgacgaagatatcctgaaatatgttgatgttggtgctacctactacttcaacaaaaacatgtccacctacg
ttgactacaaaatcaacctgctggacgacaaccagttcactcgtgacgctggcatcaacactgataacatcgtagctct
gggtctggtttaccagttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a