BBa_I728500 1 CPX CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide 2007-07-30T11:00:00Z 2015-08-31T04:07:55Z Rice JJ, Schohn A, Bessette PH, Boulware KT, and Daugherty PS. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci 2006 Apr; 15(4) 825-36. doi:10.1110/ps.051897806 pmid:16600968 This will be filled in later. false false _138_ 0 1695 9 It's complicated false Max of XXXX amino acid long peptide. true Toan Tran-Phu annotation1940506 1 Linker range1940506 1 478 489 annotation1940505 1 S54-F148 of OmpX range1940505 1 193 477 annotation1940504 1 Linker range1940504 1 175 192 annotation1975607 1 Coding sequence range1975607 1 1 654 annotation1940502 1 SfiI Restriction Site range1940502 1 70 84 annotation1940503 1 Polystyrene Sticky Peptide range1940503 1 91 129 annotation1940510 1 T7 Antibody Tag range1940510 1 142 174 annotation1940507 1 A1-S53 of OmpX range1940507 1 490 648 annotation1940501 1 Native Starting Signal range1940501 1 1 69 annotation1940508 1 Double stop codon (TGATAA) range1940508 1 649 654 annotation1940509 1 BglII Cut Site range1940509 1 85 90 annotation1940511 1 SalI Restriction Site range1940511 1 130 135 annotation1940512 1 Trypsin Cleavage Site range1940512 1 136 141 annotation1940500 1 Start codon range1940500 1 1 3 BBa_M11078 1 BBa_M11078 OmpC + Copper-Binding Protein 2009-04-17T11:00:00Z 2015-05-08T01:13:53Z Omp C is native to E.coli See Xu Z, Lee SY. (1999). Display of Polyhistidine Peptides on the Escherichia coli Cell Surface by Using Outer Membrane Protein C as an Anchoring Motif. Applied and Environmental Microbiology. 65(11): 5142-5147 Membrane display OmpC protein with inserted copper-binding protein that is expressed on the surface of Escherichia coli in an extracellular loop. false false _302_ 0 4602 302 Not in stock false note: copper-binding protein was inserted in a Pst1 restriction enzyme site ater it was filled in using DNA Polymerase. Two nucletides were inserted at the end of the copper-binding sequence to avoid throwing off the reading frame. false Forrest Purser annotation2002395 1 Copper binding Protein range2002395 1 969 1307 annotation2002430 1 start range2002430 1 1 3 annotation2002426 1 Filler range2002426 1 968 968 annotation2002431 1 stop range2002431 1 1512 1514 annotation2002425 1 Filler range2002425 1 1308 1308 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_I11010 1 BBa_I11010 RBS 2004-09-02T11:00:00Z 2015-08-31T04:07:30Z ribosome binding site false false _2_ 0 102 7 Not in stock false false bu annotation1147662 1 RBS range1147662 1 1 6 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 BBa_M11079 1 BBa_M11079 Copper binding paired with Polystyrene binding 2009-04-17T11:00:00Z 2015-05-08T01:13:53Z BioBricks found in the Registry of Standard Biological Parts were used to make this composite sequence. Contains circularly permuted OmpX protein that enables E.coli to bind to a polystyrene surface. This sequence also contains the surface display protein OmpC, which has been modified to include a copper binding protein (CutA) on the extracellular loop of the membrane protein. This system is designed to be used for removing copper from solution by adding E. coli bound to a polystyrene surface and containing the copper binding mechanism to the solution. false false _302_ 0 4602 302 Not in stock false The OmpC BioBrick would need to be mutated to remove EcoRI and a SpeI sites. There were no other special design considerations. false Forrest Purser component2002475 1 BBa_B0012 component2002456 1 BBa_B0012 component2002460 1 BBa_R0040 component2002453 1 BBa_I728500 component2002454 1 BBa_B0010 component2002432 1 BBa_R0040 component2002438 1 BBa_I11010 component2002473 1 BBa_B0010 component2002472 1 BBa_M11078 component2002466 1 BBa_I11010 annotation2002473 1 BBa_B0010 range2002473 1 2480 2559 annotation2002453 1 BBa_I728500 range2002453 1 80 733 annotation2002432 1 BBa_R0040 range2002432 1 1 54 annotation2002456 1 BBa_B0012 range2002456 1 830 870 annotation2002460 1 BBa_R0040 range2002460 1 879 932 annotation2002438 1 BBa_I11010 range2002438 1 63 73 annotation2002472 1 BBa_M11078 range2002472 1 958 2471 annotation2002454 1 BBa_B0010 range2002454 1 742 821 annotation2002475 1 BBa_B0012 range2002475 1 2568 2608 annotation2002466 1 BBa_I11010 range2002466 1 941 951 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I728500_sequence 1 atgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataa BBa_I11010_sequence 1 aggaggtcatc BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_M11079_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtcatctactagatgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtcatctactagatgaaagttaaagtactgtccctcctggtcccagctctgctggtagcaggcgcagcaaacgctgctgaagtttacaacaaagacggcaacaaattagatctgtacggtaaagtagacggcctgcactatttctctgacaacaaagatgtagatggcgaccagacctacatgcgtcttggcttcaaaggtgaaactcaggttactgaccagctgaccggttacggccagtgggaatatcagatccagggcaacagcgctgaaaacgaaaacaactcctggacccgtgtggcattcgcaggtctgaaattccaggatgtgggttctttcgactacggtcgtaactacggcgttgtttatgacgtaacttcctggaccgacgtactgccagaattcggtggtgacacctacggttctgacaacttcatgcagcagcgtggtaacggcttcgcgacctaccgtaacactgacttcttcggtctggttgacggcctgaactttgctgttcagtaccagggtaaaaacggcaacccatctggtgaaggctttactagtggcgtaactaacaacggtcgtgacgcactgcgtcaaaacggcgacggcgtcggcggttctatcacttatgattacgaaggtttcggtatcggtggtgcgatctccagctccaaacgtactgatgctcagaacaccgctgcttacatcggtaacggcgaccgtgctgaaacctacactggtggtctgaaatacgacgctaacaacatctacctggctgctcagtacacccagacctacaacgcaactcgcgtaggttccctgggttgggcgaacaaagcacagaacttcgaagctgttgctcagtaccagttcgacttcggtctgcgtccgtccctggcttacctgccaacatcaacatatttcaggatatcttcgtcgtcgtagccacgacccaggtttttacctttagactgcaaatgcttgatgaaaaaagttcgaataccgcgtctgtcgtggtgctatgtacggcaccagatgaagcgacagcccaggatttagccgccaaagtgctggcggaaaaactggcggcctgcgcgaccttgatccccggcgctacctctctctattactgggaaggtaagctggagcaagaatacgaagtgcagatgattttaaaaactaccgtatctcaccagcaggcactgctggaatgcctgaagtctcatcatccatatcaaaccccggaacttctggttttacctgttacacacggagacacagattacctctcatggctcaacgcatctttacgctgacagtctaaaggtaaaaacctgggtcgtggctacgacgacgaagatatcctgaaatatgttgatgttggtgctacctactacttcaacaaaaacatgtccacctacgttgactacaaaatcaacctgctggacgacaaccagttcactcgtgacgctggcatcaacactgataacatcgtagctctgggtctggtttaccagttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M11078_sequence 1 atgaaagttaaagtactgtccctcctggtcccagctctgctggtagcaggcgcagcaaacgctgctgaagtttacaacaaagacggcaacaaattagatctgtacggtaaagtagacggcctgcactatttctctgacaacaaagatgtagatggcgaccagacctacatgcgtcttggcttcaaaggtgaaactcaggttactgaccagctgaccggttacggccagtgggaatatcagatccagggcaacagcgctgaaaacgaaaacaactcctggacccgtgtggcattcgcaggtctgaaattccaggatgtgggttctttcgactacggtcgtaactacggcgttgtttatgacgtaacttcctggaccgacgtactgccagaattcggtggtgacacctacggttctgacaacttcatgcagcagcgtggtaacggcttcgcgacctaccgtaacactgacttcttcggtctggttgacggcctgaactttgctgttcagtaccagggtaaaaacggcaacccatctggtgaaggctttactagtggcgtaactaacaacggtcgtgacgcactgcgtcaaaacggcgacggcgtcggcggttctatcacttatgattacgaaggtttcggtatcggtggtgcgatctccagctccaaacgtactgatgctcagaacaccgctgcttacatcggtaacggcgaccgtgctgaaacctacactggtggtctgaaatacgacgctaacaacatctacctggctgctcagtacacccagacctacaacgcaactcgcgtaggttccctgggttgggcgaacaaagcacagaacttcgaagctgttgctcagtaccagttcgacttcggtctgcgtccgtccctggcttacctgccaacatcaacatatttcaggatatcttcgtcgtcgtagccacgacccaggtttttacctttagactgcaaatgcttgatgaaaaaagttcgaataccgcgtctgtcgtggtgctatgtacggcaccagatgaagcgacagcccaggatttagccgccaaagtgctggcggaaaaactggcggcctgcgcgaccttgatccccggcgctacctctctctattactgggaaggtaagctggagcaagaatacgaagtgcagatgattttaaaaactaccgtatctcaccagcaggcactgctggaatgcctgaagtctcatcatccatatcaaaccccggaacttctggttttacctgttacacacggagacacagattacctctcatggctcaacgcatctttacgctgacagtctaaaggtaaaaacctgggtcgtggctacgacgacgaagatatcctgaaatatgttgatgttggtgctacctactacttcaacaaaaacatgtccacctacgttgactacaaaatcaacctgctggacgacaaccagttcactcgtgacgctggcatcaacactgataacatcgtagctctgggtctggtttaccagttctaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z