BBa_M11079
type: ComponentDefinition
persistent identity: BBa M11079
display id: BBa_M11079
version: 1
was generated by: iGEM to SBOL conversion
description: Copper binding paired with Polystyrene binding
created: 2009-04-17T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: BioBricks found in the Registry of Standard Biological Parts were used to make this composite sequence.
owned by: james
owned by: myers
mutable description: Contains circularly permuted OmpX protein that enables E.coli to bind to a polystyrene surface. This sequence also contains the surface display protein OmpC, which has been modified to include a copper binding protein (CutA) on the extracellular loop of the membrane protein. This system is designed to be used for removing copper from solution by adding E. coli bound to a polystyrene surface and containing the copper binding mechanism to the solution.
discontinued: false
dominant: false
group u list: _302_
m user id: 0
owner id: 4602
owning group id: 302
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The OmpC BioBrick would need to be mutated to remove EcoRI and a SpeI sites. There were no other special design considerations.
star: false
creator: Forrest Purser
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I728500
component: BBa_I11010
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_M11078
sequence annotation: BBa_R0040
sequence annotation: BBa_I11010
sequence annotation: BBa_I728500
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_M11078
sequence: https://synbiohub.org/public/igem/BBa_M11079_sequence/1

BBa_I728500
type: Component
persistent identity: component2002453
display id: component2002453
version: 1
top level: BBa_M11079
definition: CPX
access: public

BBa_I11010
type: Component
persistent identity: component2002438
display id: component2002438
version: 1
top level: BBa_M11079
definition: BBa_I11010
access: public

BBa_R0040
type: Component
persistent identity: component2002432
display id: component2002432
version: 1
top level: BBa_M11079
definition: p(tetR)
access: public

BBa_I11010
type: Component
persistent identity: component2002466
display id: component2002466
version: 1
top level: BBa_M11079
definition: BBa_I11010
access: public

BBa_R0040
type: Component
persistent identity: component2002460
display id: component2002460
version: 1
top level: BBa_M11079
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component2002456
display id: component2002456
version: 1
top level: BBa_M11079
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2002454
display id: component2002454
version: 1
top level: BBa_M11079
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component2002475
display id: component2002475
version: 1
top level: BBa_M11079
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2002473
display id: component2002473
version: 1
top level: BBa_M11079
definition: BBa_B0010
access: public

BBa_M11078
type: Component
persistent identity: component2002472
display id: component2002472
version: 1
top level: BBa_M11079
definition: BBa_M11078
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2002432
display id: annotation2002432
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002432/range2002432/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M11079/annotation2002432/range2002432/1
type: Range
persistent identity: range2002432
display id: range2002432
version: 1
top level: BBa_M11079
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_I11010
type: SequenceAnnotation
persistent identity: annotation2002438
display id: annotation2002438
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002438/range2002438/1
component: BBa_I11010

https://synbiohub.org/public/igem/BBa_M11079/annotation2002438/range2002438/1
type: Range
persistent identity: range2002438
display id: range2002438
version: 1
top level: BBa_M11079
direction: direction/reverse
start: 63
end: 73
orientation: inline

BBa_I728500
type: SequenceAnnotation
persistent identity: annotation2002453
display id: annotation2002453
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002453/range2002453/1
component: BBa_I728500

https://synbiohub.org/public/igem/BBa_M11079/annotation2002453/range2002453/1
type: Range
persistent identity: range2002453
display id: range2002453
version: 1
top level: BBa_M11079
direction: direction/reverse
start: 80
end: 733
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2002454
display id: annotation2002454
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002454/range2002454/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M11079/annotation2002454/range2002454/1
type: Range
persistent identity: range2002454
display id: range2002454
version: 1
top level: BBa_M11079
direction: direction/forward
start: 742
end: 821
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2002456
display id: annotation2002456
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002456/range2002456/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M11079/annotation2002456/range2002456/1
type: Range
persistent identity: range2002456
display id: range2002456
version: 1
top level: BBa_M11079
direction: direction/forward
start: 830
end: 870
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2002460
display id: annotation2002460
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002460/range2002460/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M11079/annotation2002460/range2002460/1
type: Range
persistent identity: range2002460
display id: range2002460
version: 1
top level: BBa_M11079
direction: direction/forward
start: 879
end: 932
orientation: inline

BBa_I11010
type: SequenceAnnotation
persistent identity: annotation2002466
display id: annotation2002466
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002466/range2002466/1
component: BBa_I11010

https://synbiohub.org/public/igem/BBa_M11079/annotation2002466/range2002466/1
type: Range
persistent identity: range2002466
display id: range2002466
version: 1
top level: BBa_M11079
direction: direction/reverse
start: 941
end: 951
orientation: inline

BBa_M11078
type: SequenceAnnotation
persistent identity: annotation2002472
display id: annotation2002472
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002472/range2002472/1
component: BBa_M11078

https://synbiohub.org/public/igem/BBa_M11079/annotation2002472/range2002472/1
type: Range
persistent identity: range2002472
display id: range2002472
version: 1
top level: BBa_M11079
direction: direction/reverse
start: 958
end: 2471
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2002473
display id: annotation2002473
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002473/range2002473/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M11079/annotation2002473/range2002473/1
type: Range
persistent identity: range2002473
display id: range2002473
version: 1
top level: BBa_M11079
direction: direction/forward
start: 2480
end: 2559
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2002475
display id: annotation2002475
version: 1
top level: BBa_M11079
location: https://synbiohub.org/public/igem/BBa_M11079/annotation2002475/range2002475/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M11079/annotation2002475/range2002475/1
type: Range
persistent identity: range2002475
display id: range2002475
version: 1
top level: BBa_M11079
direction: direction/forward
start: 2568
end: 2608
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

CPX
type: ComponentDefinition
persistent identity: BBa I728500
display id: BBa_I728500
version: 1
was generated by: iGEM to SBOL conversion
description: CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide
created: 2007-07-30T11:00:00Z
modified: 2015-08-31T04:07:55Z
mutable provenance: Rice JJ, Schohn A, Bessette PH, Boulware KT, and Daugherty PS. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci 2006 Apr; 15(4) 825-36. doi:10.1110/ps.051897806 pmid:16600968
owned by: james
owned by: myers
mutable description: This will be filled in later.
discontinued: false
dominant: false
experience: experience/Works
group u list: _138_
m user id: 0
owner id: 1695
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Max of XXXX amino acid long peptide.
star: true
creator: Toan Tran-Phu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start codon
sequence annotation: Native Starting Signal
sequence annotation: Coding sequence
sequence annotation: SfiI Restriction Site
sequence annotation: BglII Cut Site
sequence annotation: Polystyrene Sticky Peptide
sequence annotation: SalI Restriction Site
sequence annotation: Trypsin Cleavage Site
sequence annotation: T7 Antibody Tag
sequence annotation: Linker
sequence annotation: S54-F148 of OmpX
sequence annotation: A1-S53 of OmpX
sequence annotation: Double stop codon (TGATAA)
sequence: https://synbiohub.org/public/igem/BBa_I728500_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation1940500
display id: annotation1940500
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940500/range1940500/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I728500/annotation1940500/range1940500/1
type: Range
persistent identity: range1940500
display id: range1940500
version: 1
top level: CPX
direction: direction/forward
start: 1
end: 3
orientation: inline

Native Starting Signal
type: SequenceAnnotation
persistent identity: annotation1940501
display id: annotation1940501
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940501/range1940501/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_I728500/annotation1940501/range1940501/1
type: Range
persistent identity: range1940501
display id: range1940501
version: 1
top level: CPX
direction: direction/forward
start: 1
end: 69
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1975607
display id: annotation1975607
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1975607/range1975607/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I728500/annotation1975607/range1975607/1
type: Range
persistent identity: range1975607
display id: range1975607
version: 1
top level: CPX
direction: direction/forward
start: 1
end: 654
orientation: inline

SfiI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1940502
display id: annotation1940502
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940502/range1940502/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_I728500/annotation1940502/range1940502/1
type: Range
persistent identity: range1940502
display id: range1940502
version: 1
top level: CPX
direction: direction/forward
start: 70
end: 84
orientation: inline

BglII Cut Site
type: SequenceAnnotation
persistent identity: annotation1940509
display id: annotation1940509
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940509/range1940509/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I728500/annotation1940509/range1940509/1
type: Range
persistent identity: range1940509
display id: range1940509
version: 1
top level: CPX
direction: direction/forward
start: 85
end: 90
orientation: inline

Polystyrene Sticky Peptide
type: SequenceAnnotation
persistent identity: annotation1940503
display id: annotation1940503
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940503/range1940503/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_I728500/annotation1940503/range1940503/1
type: Range
persistent identity: range1940503
display id: range1940503
version: 1
top level: CPX
direction: direction/forward
start: 91
end: 129
orientation: inline

SalI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1940511
display id: annotation1940511
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940511/range1940511/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I728500/annotation1940511/range1940511/1
type: Range
persistent identity: range1940511
display id: range1940511
version: 1
top level: CPX
direction: direction/forward
start: 130
end: 135
orientation: inline

Trypsin Cleavage Site
type: SequenceAnnotation
persistent identity: annotation1940512
display id: annotation1940512
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940512/range1940512/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I728500/annotation1940512/range1940512/1
type: Range
persistent identity: range1940512
display id: range1940512
version: 1
top level: CPX
direction: direction/forward
start: 136
end: 141
orientation: inline

T7 Antibody Tag
type: SequenceAnnotation
persistent identity: annotation1940510
display id: annotation1940510
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940510/range1940510/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_I728500/annotation1940510/range1940510/1
type: Range
persistent identity: range1940510
display id: range1940510
version: 1
top level: CPX
direction: direction/forward
start: 142
end: 174
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation1940504
display id: annotation1940504
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940504/range1940504/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I728500/annotation1940504/range1940504/1
type: Range
persistent identity: range1940504
display id: range1940504
version: 1
top level: CPX
direction: direction/forward
start: 175
end: 192
orientation: inline

S54-F148 of OmpX
type: SequenceAnnotation
persistent identity: annotation1940505
display id: annotation1940505
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940505/range1940505/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_I728500/annotation1940505/range1940505/1
type: Range
persistent identity: range1940505
display id: range1940505
version: 1
top level: CPX
direction: direction/forward
start: 193
end: 477
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation1940506
display id: annotation1940506
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940506/range1940506/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I728500/annotation1940506/range1940506/1
type: Range
persistent identity: range1940506
display id: range1940506
version: 1
top level: CPX
direction: direction/forward
start: 478
end: 489
orientation: inline

A1-S53 of OmpX
type: SequenceAnnotation
persistent identity: annotation1940507
display id: annotation1940507
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940507/range1940507/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I728500/annotation1940507/range1940507/1
type: Range
persistent identity: range1940507
display id: range1940507
version: 1
top level: CPX
direction: direction/forward
start: 490
end: 648
orientation: inline

Double stop codon (TGATAA)
type: SequenceAnnotation
persistent identity: annotation1940508
display id: annotation1940508
version: 1
top level: CPX
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940508/range1940508/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I728500/annotation1940508/range1940508/1
type: Range
persistent identity: range1940508
display id: range1940508
version: 1
top level: CPX
direction: direction/forward
start: 649
end: 654
orientation: inline

BBa_I11010
type: ComponentDefinition
persistent identity: BBa I11010
display id: BBa_I11010
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: ribosome binding site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I11010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1147662
display id: annotation1147662
version: 1
top level: BBa_I11010
location: https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
type: Range
persistent identity: range1147662
display id: range1147662
version: 1
top level: BBa_I11010
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_M11078
type: ComponentDefinition
persistent identity: BBa M11078
display id: BBa_M11078
version: 1
was generated by: iGEM to SBOL conversion
description: OmpC + Copper-Binding Protein
created: 2009-04-17T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: Omp C is native to E.coli See Xu Z, Lee SY. (1999). Display of Polyhistidine Peptides on the Escherichia coli Cell Surface by Using Outer Membrane Protein C as an Anchoring Motif. Applied and Environmental Microbiology. 65(11): 5142-5147
owned by: james
owned by: myers
mutable description: Membrane display OmpC protein with inserted copper-binding protein that is expressed on the surface of Escherichia coli in an extracellular loop.
discontinued: false
dominant: false
group u list: _302_
m user id: 0
owner id: 4602
owning group id: 302
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: note: copper-binding protein was inserted in a Pst1 restriction enzyme site ater it was filled in using DNA Polymerase. Two nucletides were inserted at the end of the copper-binding sequence to avoid throwing off the reading frame.
star: false
creator: Forrest Purser
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Filler
sequence annotation: Copper binding Protein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M11078_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2002430
display id: annotation2002430
version: 1
top level: BBa_M11078
location: https://synbiohub.org/public/igem/BBa_M11078/annotation2002430/range2002430/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M11078/annotation2002430/range2002430/1
type: Range
persistent identity: range2002430
display id: range2002430
version: 1
top level: BBa_M11078
direction: direction/forward
start: 1
end: 3
orientation: inline

Filler
type: SequenceAnnotation
persistent identity: annotation2002426
display id: annotation2002426
version: 1
top level: BBa_M11078
location: https://synbiohub.org/public/igem/BBa_M11078/annotation2002426/range2002426/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M11078/annotation2002426/range2002426/1
type: Range
persistent identity: range2002426
display id: range2002426
version: 1
top level: BBa_M11078
direction: direction/forward
start: 968
end: 968
orientation: inline

Copper binding Protein
type: SequenceAnnotation
persistent identity: annotation2002395
display id: annotation2002395
version: 1
top level: BBa_M11078
location: https://synbiohub.org/public/igem/BBa_M11078/annotation2002395/range2002395/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M11078/annotation2002395/range2002395/1
type: Range
persistent identity: range2002395
display id: range2002395
version: 1
top level: BBa_M11078
direction: direction/forward
start: 969
end: 1307
orientation: inline

Filler
type: SequenceAnnotation
persistent identity: annotation2002425
display id: annotation2002425
version: 1
top level: BBa_M11078
location: https://synbiohub.org/public/igem/BBa_M11078/annotation2002425/range2002425/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M11078/annotation2002425/range2002425/1
type: Range
persistent identity: range2002425
display id: range2002425
version: 1
top level: BBa_M11078
direction: direction/forward
start: 1308
end: 1308
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2002431
display id: annotation2002431
version: 1
top level: BBa_M11078
location: https://synbiohub.org/public/igem/BBa_M11078/annotation2002431/range2002431/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M11078/annotation2002431/range2002431/1
type: Range
persistent identity: range2002431
display id: range2002431
version: 1
top level: BBa_M11078
direction: direction/forward
start: 1512
end: 1514
orientation: inline

https://synbiohub.org/public/igem/BBa_M11079_sequence/1
type: Sequence
persistent identity: BBa M11079 sequence
display id: BBa_M11079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtcatctactagatgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtcatctactagatgaaagttaaagtactgtccctcctggtcccagctctgctggtagcaggcgcagcaaacgctgctgaagtttacaacaaagacggcaacaaattagatctgtacggtaaagtagacggcctgcactatttctctgacaacaaagatgtagatggcgaccagacctacatgcgtcttggcttcaaaggtgaaactcaggttactgaccagctgaccggttacggccagtgggaatatcagatccagggcaacagcgctgaaaacgaaaacaactcctggacccgtgtggcattcgcaggtctgaaattccaggatgtgggttctttcgactacggtcgtaactacggcgttgtttatgacgtaacttcctggaccgacgtactgccagaattcggtggtgacacctacggttctgacaacttcatgcagcagcgtggtaacggcttcgcgacctaccgtaacactgacttcttcggtctggttgacggcctgaactttgctgttcagtaccagggtaaaaacggcaacccatctggtgaaggctttactagtggcgtaactaacaacggtcgtgacgcactgcgtcaaaacggcgacggcgtcggcggttctatcacttatgattacgaaggtttcggtatcggtggtgcgatctccagctccaaacgtactgatgctcagaacaccgctgcttacatcggtaacggcgaccgtgctgaaacctacactggtggtctgaaatacgacgctaacaacatctacctggctgctcagtacacccagacctacaacgcaactcgcgtaggttccctgggttgggcgaacaaagcacagaacttcgaagctgttgctcagtaccagttcgacttcggtctgcgtccgtccctggcttacctgccaacatcaacatatttcaggatatcttcgtcgtcgtagccacgacccaggtttttacctttagactgcaaatgcttgatgaaaaaagttcgaataccgcgtctgtcgtggtgctatgtacggcaccagatgaagcgacagcccag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I728500_sequence/1
type: Sequence
persistent identity: BBa I728500 sequence
display id: BBa_I728500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11010_sequence/1
type: Sequence
persistent identity: BBa I11010 sequence
display id: BBa_I11010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggtcatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M11078_sequence/1
type: Sequence
persistent identity: BBa M11078 sequence
display id: BBa_M11078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagttaaagtactgtccctcctggtcccagctctgctggtagcaggcgcagcaaacgctgctgaagtttacaacaaagacggcaacaaattagatctgtacggtaaagtagacggcctgcactatttctctgacaacaaagatgtagatggcgaccagacctacatgcgtcttggcttcaaaggtgaaactcaggttactgaccagctgaccggttacggccagtgggaatatcagatccagggcaacagcgctgaaaacgaaaacaactcctggacccgtgtggcattcgcaggtctgaaattccaggatgtgggttctttcgactacggtcgtaactacggcgttgtttatgacgtaacttcctggaccgacgtactgccagaattcggtggtgacacctacggttctgacaacttcatgcagcagcgtggtaacggcttcgcgacctaccgtaacactgacttcttcggtctggttgacggcctgaactttgctgttcagtaccagggtaaaaacggcaacccatctggtgaaggctttactagtggcgtaactaacaacggtcgtgacgcactgcgtcaaaacggcgacggcgtcggcggttctatcacttatgattacgaaggtttcggtatcggtggtgcgatctccagctccaaacgtactgatgctcagaacaccgctgcttacatcggtaacggcgaccgtgctgaaacctacactggtggtctgaaatacgacgctaacaacatctacctggctgctcagtacacccagacctacaacgcaactcgcgtaggttccctgggttgggcgaacaaagcacagaacttcgaagctgttgctcagtaccagttcgacttcggtctgcgtccgtccctggcttacctgccaacatcaacatatttcaggatatcttcgtcgtcgtagccacgacccaggtttttacctttagactgcaaatgcttgatgaaaaaagttcgaataccgcgtctgtcgtggtgctatgtacggcaccagatgaagcgacagcccaggatttagccgccaaagtgctggcggaaaaactggcggcctgcgcgaccttgatccccggcgctacctctctctattactgggaaggtaagctggagcaagaatacgaagtgcagatgattttaaaaactaccgtatctcaccagcaggcactgctggaatgcctgaagtctcatcatccatatcaaaccccggaacttctggttttacctgttacacacggagacacagattacctctcatggctcaacgcatctttacgctgacagtctaaaggtaaaaacctgggtcgtggctacgacgacgaagatatcctgaaatatgttgatgttggtgctacctactacttcaacaaaaacatgtccacctacgttgactacaaaatcaacctgctggacgacaaccagttcactcgtgacgctggcatcaacactgataacatcgtagctctgggtctggtttaccagttctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z