BBa_M11084 1 ompC w/Cad E coli outer membrane protein C (ompC) with Cadmium Binding protien 2009-04-17T11:00:00Z 2015-05-08T01:13:53Z The source of the ompC gene is: Escherichia coli SMS-3-5 DNA Coordinates 2395366..2396460 (-)(1095bp) OmpC is normally found in the outer membrane of E. coli. Coding for a Cadmium binding protein was inserted on the 9th hydrophylic loop which protrudes out of the cell. The sequence This biobrick would be useful for the binding cadmium to the cell wall of E. coli. It could be applicable to water treatment applications. false false _302_ 0 4574 302 Not in stock true The 9th hydrophylic loop protrudes out of the cell and normally contains a PstI RE site just after the loop exits the outer membrane lipid layer. The PstI site 886bp in to the ompC sequence and it is in the 9th loop. To make this biobrick the the PstI site at 638bp and the EcoRI site at 388bp of the gene was inactivated by mutation during synthesis. false Jason D. Brown annotation2002571 1 start range2002571 1 1 3 annotation2002576 1 stop range2002576 1 1711 1713 annotation2002572 1 1st half ompC range2002572 1 4 888 annotation2002574 1 2nd half ompC range2002574 1 1509 1707 annotation2002573 1 Cadmium Binding Protein range2002573 1 889 1508 annotation2002575 1 stop range2002575 1 1708 1710 BBa_M11084_sequence 1 atgaaagttaaagtactgtccctcctggtcccagctctgctggtagcaggcgcagcaaacgctgctgaaatttacaacaaagacggcaacaaattagatctgtacggtaaagttgacggcctgcactatttctccgataacgactctaaagatggcgacaagacctacatgcgtcttggcttcaaaggtgaaactcaggttactgatcaactgaccggttatggtcagtgggaatatcagattcaaggtaacgaacctgaaagtgacaatagttcctggacccgtgtggcattcgctggtctgaaattccaggacgttggttcttttgactatggtcgtaactacggcgttgtttacgatgttacttcctggaccgacgtactgccagacttcggtggtgatacctacgattctgataacttcatgcagcagcgtggtaacggtttcgcgacctaccgtaacaccgacttcttcggtctggttgacggtcttgattttgctgttcagtatcagggtaaaaacggtagtgctcatggcgaaggtatgaccactaatggccgtgatgatgtatttgagcagaacggtgacggtgttggtggttctattacttacaattatgaaggctttggtatcggtgctacagtttccagctccaaacgtacctgggatcagaacaacactggcctgatcggtactggcgaccgtgctgaaacctacactggtggtctgaaatacgacgctaacaacatctacctggctgctcagtacacccagacctacaacgcaactcgcgtaggttccctgggttgggcgaacaaagcacagaacttcgaagctgttgctcagtaccagttcgacttcggtctgcgtccgtccctggcttacctgatgattcaaacggttgttgctgctgctgttctttatattgctacagcagttgatttattagtaattttattaatattttttgctagagcaaagactagaaaagaatatagagatatttatgttggtcaatatttagggtctattattttaatattagttagtttgtttttagcttttgtattaaattatgttccagaaaagtggatattaggtttattgggtttaataccaatttatcttggaattaaagtggctatttatgatgattgtgaaggagaaaagagagctaaaaaagaattgaatgaaaaaggattgtctaaattagttggtacggttgcaattgttacgatagcaagttgtggcgccgataatattggtttatttgttccgtattttgtgacattaagtgttactaatttattacttactctgtttgtctttttaattttaattttcttcttggtgtttactgcacaaaaattagctaatattccaggaattggagaaattgttgagaaatttagtcgttggattatggctattatttatatagctttaggtttatttattattattgaaaatgacactattcaaacaattttaggatttatatttcagtctaaaggtaaaaacctgggtcgtggctacgacgacgaagatatcctgaaatatgttgatgttggtgcgacctactacttcaacaaaaacatgtccacctacgttgactacaaaatcaacctgctggacgacaaccagttcacccgcgctgctggcatcaacactgatgacatcgtagctctgggtctggtttaccagttctaatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z