LOCUS BBa_M1110 5376 bp DNA linear 22-Nov-2024 DEFINITION Complete Pyrene Degradation pathway ACCESSION BBa_M1110 VERSION BBa_M1110.1 FEATURES Location/Qualifiers misc_feature 1..22 /label=BBa_M1905 promoter 218..223 /label=-10 promoter 194..199 /label=-35 promoter 31..229 /label=BBa_J45992 RBS 238..249 /label=BBa_J61100 misc_feature 256..1623 /label=BBa_M1106 RBS 1632..1643 /label=BBa_J61100 misc_feature 1650..2159 /label=BBa_M1107 RBS 2168..2179 /label=BBa_J61100 misc_feature 2259..2261 /label=start codon CDS 2259..2312 /label=coding sequence misc_feature 2310..2312 /label=stop codon misc_feature 2188..2337 /label=BBa_M1108 RBS 2346..2357 /label=BBa_J61100 misc_feature 2437..2439 /label=start codon CDS 2437..2490 /label=coding sequence misc_feature 2488..2490 /label=stop codon misc_feature 2366..2515 /label=BBa_M1108 RBS 2524..2535 /label=BBa_J61100 misc_feature 2544..2546 /label=start CDS 2544..4094 /label=coding portion misc_feature 4092..4094 /label=Stop misc_feature 4094..4096 /label=Stop misc_feature 2544..4097 /label=BBa_M1102 RBS 4106..4117 /label=BBa_J61100 misc_feature 4124..4642 /label=BBa_M1103 RBS 4651..4662 /label=BBa_J61100 misc_feature 4671..4673 /label=Start CDS 4671..5207 /label=CDS misc_feature 5205..5207 /label=Stp misc_feature 5208..5210 /label=Stp misc_feature 4671..5210 /label=BBa_M1104 stem_loop 5230..5273 /label=stem_loop misc_feature 5219..5298 /label=BBa_B0010 terminator 5219..5298 /label=BBa_B0010 stem_loop 5314..5333 /label=T7 TE misc_feature 5340..5340 /label=stop polyA_site 5334..5347 /label=polya misc_feature 5307..5347 /label=BBa_B0012 terminator 5307..5347 /label=BBa_B0012 misc_feature 5356..5376 /label=BBa_M1906 ORIGIN 1 gaattcgcgg ccgcttctag agtactagag ctggcacagg aacgttatcc ggacgttcag 61 ttccaccaga cccgcgagca ttaattcttg cctccagggc gcggtagtgg cgccctgtca 121 atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt 181 tttcgctgat atcccgagcg gtttcaaaat tgtgatctat atttaacaat actagagaaa 241 gaggggacat actagatgac aaccgaaacc acaggtacag cagatgcaac tgatccgtat 301 ctgcgtcggg cccttcggga ggtagccgat ggcttgaaag taggtcgtct tcccgcgagg 361 gtggttagcg accctgccct gcatactatc gaaatggaac gtattttcgg ccgtgcttgg 421 gtctttctgg ggcatgaatc cgagttggcc aaaagtggag actttgtcgt aaggcatatc 481 ggagcagact cagtgatagt ttgccgcgat aactcaggac ggattcaggc cctgtcaaat 541 tcctgtcgtc accgaggcgc cctggtgtgt cgtgctgaaa tgggtaacac tgcgcacttt 601 caatgcccat atcatgggtg ggtctactcg aacaccggag agctggtggg tgtcccggct 661 atgtcagagg cgtacccagg cggttttgat aaatctcagt gggggctgcg acacattccg 721 catgttgact catatgctgg ctttattttt ggcagtgtag atcctaaagc accaagtttg 781 acagactatc tgggagatac tactttctat ctggatttga ttgctaagaa aaccgcaggt 841 ggcctagagg tcataggggc tccgcatcgt tgggttatgt cagccaattg gaagacggcg 901 gccgacaact ttgtgggtga tagctaccac accttattcg cccatcggtc catggttgaa 961 ttagggatgg ccccgggaga tccaaatttc gctagtgccc cggctgaaat tagcttgcaa 1021 aatggtcatg gcgtgggagt cttgggattt cccccaaccc ttgcggattt cccagaatac 1081 gagggttacc ctgatgaagt tgttgatcag atggcgacga gctacccatc cccggtacat 1141 aaggatctta tgcgtaggtc ttccttcatt catggtacag tttttcctaa tctttcattt 1201 attaatgtga cattagcaca agatcatatg tcgccgccaa ccccatttat cacttttcgt 1261 gtttggcacc cgctgagcca tgaccgcatg gagatactgt catggttttt agttgagcgg 1321 gacgccccgg agtggctacg cgatgcttcg caagcgagct acgtgaacaa cttcggtcct 1381 gggggcgtat tcgagcagga tgacgcagaa gcatggaaag ccatcaccga atccgtccag 1441 ggaccgtttg ccggtgaagg cctgcttaac tatgaaatgg gcatggatct cacccctctg 1501 acagattggc ccgggccggg tgaagctttg ccgtctggtt atgctgaaca gaatcaaaga 1561 cggttttggg gccgatggct ggaatacatg ggccagcctc cggcatttgg aggcagggca 1621 tgatactaga gaaagagggg acatactaga tgaacgcggt ggctgttgac caggatactc 1681 gcgaagcggt tgaggagttc atgttccgtg aagctgaact gctcgacgga ggacaattcc 1741 gcgagtggct gggcttgtta gaccctgata tcagatatgt cgtgcctgtg cggacgactc 1801 gcgaagactc cgcaggatgg gtgggtgcaa tcgcacactg gaatgatgat tacaccggcc 1861 tggaaatgcg ggttctgcgc ggcgagaccg atttttcgtg ggctgaaagc ccacgttctc 1921 gtactcgtca ttttgttagt aatatccgga ctacagccgg tccagaagct gatgaattaa 1981 cggtgcgtag caatcttctc ttcttcaggt cgcgcggcga ttcgggccgc tgggaactgt 2041 tatcagccga acgtgtggac gttcttcgcc gcacggatga tagcctgcgg cttgccagaa 2101 gagaagttct cctagaccat tctacccttc ctattgacaa tttatcagtg tttttatagt 2161 actagagaaa gaggggacat actagagttt tggatatgga aaaacaaaag aagaagaaat 2221 gtgaatagaa ggttggataa agaagctaat aaaaggggat gatattgagg aactggaaat 2281 aggacataaa gaaggtgatg gggtatagat aagagttaat gtaagagggg ggcatgttac 2341 tagagaaaga ggggacatac tagagttttg gatatggaaa aacaaaagaa gaagaaatgt 2401 gaatagaagg ttggataaag aagctaataa aaggggatga tattgaggaa ctggaaatag 2461 gacataaaga aggtgatggg gtatagataa gagttaatgt aagagggggg catgttacta 2521 gagaaagagg ggacatacta gaggtgtccg atgcacaggt agccaagacc gacgtcgtgc 2581 tggtcggtgc aggcatcatg agcgccacgc tgagcgccct gatcaagctg ctcgagccca 2641 actggtcgat caccctgatc gagcggctcg acggcgccgc cgccgagagc agcgacccct 2701 ggaacaacgc aggcaccggg cactccgcgc tgtgcgagct caactacacc ccggaggggc 2761 ccggcgggtc gatcgacatc accaaagcgg tgcacgtcaa cgagcagttc caggtgtcgc 2821 gacagttctg gacgtacgcc gtggagaacg gcgtgctgcc cgatgtgcgc aacttcatca 2881 acccgatccc gcacgtcagc ttcgtcagcg gtgcaaggaa cgtggagtac ctgcgcgccc 2941 gttacgacgc gttggtcccc aacccgctgt tcgcgacgat ggagtacatc gacgaccgcg 3001 acgagttcgc ccggcggctg ccgttcatgg ccgacaaacg cgatttccgc gagccggtcg 3061 cgctgaactg gtcccaggac ggcaccgaca tcgatttcgg ctcgctgtcg cggcagctga 3121 tcggctacac ggcccagcgg ggcatgacga cgctgttcgg tcacgaggtc cgcgacctgg 3181 acaagcagtc cgacggcagc tggtcggtca aggtggtcaa ccgccgcacc ggcgccaagc 3241 gcaagctcaa cgcgaagttc gtcttcgtcg gcgcgggcgg cggcgcgctg ccgctgctcc 3301 agaaggccgg gatcgaggag gccaagggct tcggcggctt cccggtgggc gggcagtggt 3361 tgcgcaccgg caatcccgag ctcaccgcgc gccaccaggc caaggtgtac gggatgccgc 3421 ccctgggcgc gccgccgatg tcggtgcccc acctcgacac gcgcgtcatc aacgggaagt 3481 cctggctgct gttcggtccg ttcgcgggct ggtcgccgaa gttcctcaaa cagggcaagg 3541 tcaccgatct gccgttctcg gtcaagccga acaacctggc gtcgatgctg ggtgtcgggc 3601 tgaccgagat gggcctgctc aagtacctga tcggccagct gctactcagc gaggccgacc 3661 gggtggagac gctgcgcaac ttcgccccca gcgcacggga ctccgactgg gagctcgaca 3721 tcgccggtca gcgggtgcag gtgatccgcc gcaagggcaa gggcggcgtg ctggagttcg 3781 gcaccacggt cctggccgcc aaggacggca gcatcgcagg cctgctcggg gcgtcgccgg 3841 gggcgtcgac cgccgtgccc gcgatgctcg atgtcatgga gcgttgcttc ggcggccggt 3901 acaccgcctg gctgccgaag ctcaaggaaa tcgtgccgtc gctgggcacc aagctctccg 3961 acgagccgaa gctgttccag gagatctggg cgcacgggac gaaggtgctc aagctggacc 4021 acccggccgc ggggctgccg gtcgccggaa ccgacaccga acaccgtgag cccgcaacca 4081 cggtgaccgc ctgatgatac tagagaaaga ggggacatac tagatggcag cttctccacg 4141 gtggcagtgc gaccgggcgg cgggcgcgtc cggggccatt cagtttcacg gggtgattac 4201 ggatgaagat ggtcgaccag ttcctaacgt cctgatcgag acctggcacg ccgcggggga 4261 cgacgacaac gcggcgacga agatcggacg gccgcggtgc gacggccgga actacacccg 4321 cccaaagagc gtcatcaccg accaacaggg tcgttacagc atgagaatgg tgccgccacg 4381 agcacccgag cacggggcag cgccgttcat tgcggtgtcg gtgcatgcac gagggcttct 4441 cgatcggctg ataacccgcg cctaccttcc agggtgccat ctcgccaagg accccctgct 4501 ccgccgcctg cccgccgagc ggcggcaagc cctgattgcc acccgcgacg acatcggact 4561 tcgtttcgac atcacgctgc ggccagcaca cggcgtgagg gttgaaacct ttgttgagcc 4621 gcaacaggaa gctgcgacgt gatactagag aaagagggga catactagag gtgacgcgta 4681 tcgcacccgg cggattccgg gaactgggac ccctgaactg ggccatcgcg aaggcgggtg 4741 cccgtgccat ccgcgcgccg cgcttcagcc tgttcaacgt gctcggccaa caccgtctgc 4801 tgttcctggc gttcctgccc tacagcggtg tgctgctcgg ccgcctcggc aagctgtccc 4861 gtcaggacgc cgaactcgtg atcctgcggg tcggccatct gcgcgagtgt gagtacgaac 4921 tccagcagca ccgacggctg gcccgcagcc gcggcgtcgg cgcggacctt caggcccgga 4981 tcttcgaggg ccccgacgcc gagggtctca ccgaccggca gcgggtgctc gtcaccgcca 5041 ccgacgagtt cgtcgtcacc cgaggggtgt caccgcagac gtggtcggtg ctggcccggc 5101 atctcaccaa acctcagctc atcgagttct gcctgctggc agcgcattac gacggtctgg 5161 cggcgacgat cagcacgctc caggtgccgc tcgacttccc ggactgatga tactagagcc 5221 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 5281 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 5341 gtttatatac tagagtacta gtagcggccg ctgcag //