##gff-version 3
##sequence-region BBa_M1110 1 5376
BBa_M1110	.	promoter	31	229	.	+	0	ID=BBa_J45992;Name=BBa_J45992
BBa_M1110	.	promoter	218	223	.	+	0	ID=annotation1883645;Name=-10;Parent=BBa_J45992
BBa_M1110	.	promoter	194	199	.	+	0	ID=annotation1883646;Name=-35;Parent=BBa_J45992
BBa_M1110	.	ribosome_entry_site	2168	2179	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	sequence_feature	1	22	.	+	0	ID=BBa_M1905;Name=BBa_M1905
BBa_M1110	.	polypeptide_domain	256	1623	.	+	0	ID=BBa_M1106;Name=BBa_M1106
BBa_M1110	.	polypeptide_domain	2544	4097	.	+	0	ID=BBa_M1102;Name=BBa_M1102
BBa_M1110	.	start_codon	2544	2546	.	+	0	ID=annotation2172385;Name=start;Parent=BBa_M1102
BBa_M1110	.	stop_codon	4094	4096	.	+	0	ID=annotation2172388;Name=Stop;Parent=BBa_M1102
BBa_M1110	.	stop_codon	4092	4094	.	+	0	ID=annotation2172387;Name=Stop;Parent=BBa_M1102
BBa_M1110	.	CDS	2544	4094	.	+	0	ID=annotation2172386;Name=coding portion ;Parent=BBa_M1102
BBa_M1110	.	polypeptide_domain	2188	2337	.	+	0	ID=BBa_M1108;Name=BBa_M1108
BBa_M1110	.	CDS	2259	2312	.	+	0	ID=annotation2173800;Name=coding sequence ;Parent=BBa_M1108
BBa_M1110	.	stop_codon	2310	2312	.	+	0	ID=annotation2173801;Name=stop codon;Parent=BBa_M1108
BBa_M1110	.	start_codon	2259	2261	.	+	0	ID=annotation2173799;Name=start codon ;Parent=BBa_M1108
BBa_M1110	.	ribosome_entry_site	2346	2357	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	ribosome_entry_site	4651	4662	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	ribosome_entry_site	1632	1643	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	terminator	5307	5347	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1110	.	polyA_site	5334	5347	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1110	.	stem_loop	5314	5333	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1110	.	engineered_region	5307	5347	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1110	.	stop_codon	5340	5340	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1110	.	polypeptide_domain	4124	4642	.	+	0	ID=BBa_M1103;Name=BBa_M1103
BBa_M1110	.	sequence_feature	5356	5376	.	+	0	ID=BBa_M1906;Name=BBa_M1906
BBa_M1110	.	ribosome_entry_site	2524	2535	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	polypeptide_domain	1650	2159	.	+	0	ID=BBa_M1107;Name=BBa_M1107
BBa_M1110	.	terminator	5219	5298	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1110	.	stem_loop	5230	5273	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1110	.	engineered_region	5219	5298	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1110	.	ribosome_entry_site	4106	4117	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	polypeptide_domain	4671	5210	.	+	0	ID=BBa_M1104;Name=BBa_M1104
BBa_M1110	.	stop_codon	5205	5207	.	+	0	ID=annotation2172391;Name=Stp;Parent=BBa_M1104
BBa_M1110	.	start_codon	4671	4673	.	+	0	ID=annotation2172389;Name=Start;Parent=BBa_M1104
BBa_M1110	.	CDS	4671	5207	.	+	0	ID=annotation2172390;Name=CDS;Parent=BBa_M1104
BBa_M1110	.	stop_codon	5208	5210	.	+	0	ID=annotation2172392;Name=Stp;Parent=BBa_M1104
BBa_M1110	.	ribosome_entry_site	238	249	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1110	.	polypeptide_domain	2366	2515	.	+	0	ID=BBa_M1108;Name=BBa_M1108
BBa_M1110	.	CDS	2437	2490	.	+	0	ID=annotation2173800;Name=coding sequence ;Parent=BBa_M1108
BBa_M1110	.	stop_codon	2488	2490	.	+	0	ID=annotation2173801;Name=stop codon;Parent=BBa_M1108
BBa_M1110	.	start_codon	2437	2439	.	+	0	ID=annotation2173799;Name=start codon ;Parent=BBa_M1108
>BBa_M1110
gaattcgcggccgcttctagagtactagagctggcacaggaacgttatccggacgttcagttccaccagacccgcgagc
attaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttctta
aaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagag
aaagaggggacatactagatgacaaccgaaaccacaggtacagcagatgcaactgatccgtatctgcgtcgggcccttc
gggaggtagccgatggcttgaaagtaggtcgtcttcccgcgagggtggttagcgaccctgccctgcatactatcgaaat
ggaacgtattttcggccgtgcttgggtctttctggggcatgaatccgagttggccaaaagtggagactttgtcgtaagg
catatcggagcagactcagtgatagtttgccgcgataactcaggacggattcaggccctgtcaaattcctgtcgtcacc
gaggcgccctggtgtgtcgtgctgaaatgggtaacactgcgcactttcaatgcccatatcatgggtgggtctactcgaa
caccggagagctggtgggtgtcccggctatgtcagaggcgtacccaggcggttttgataaatctcagtgggggctgcga
cacattccgcatgttgactcatatgctggctttatttttggcagtgtagatcctaaagcaccaagtttgacagactatc
tgggagatactactttctatctggatttgattgctaagaaaaccgcaggtggcctagaggtcataggggctccgcatcg
ttgggttatgtcagccaattggaagacggcggccgacaactttgtgggtgatagctaccacaccttattcgcccatcgg
tccatggttgaattagggatggccccgggagatccaaatttcgctagtgccccggctgaaattagcttgcaaaatggtc
atggcgtgggagtcttgggatttcccccaacccttgcggatttcccagaatacgagggttaccctgatgaagttgttga
tcagatggcgacgagctacccatccccggtacataaggatcttatgcgtaggtcttccttcattcatggtacagttttt
cctaatctttcatttattaatgtgacattagcacaagatcatatgtcgccgccaaccccatttatcacttttcgtgttt
ggcacccgctgagccatgaccgcatggagatactgtcatggtttttagttgagcgggacgccccggagtggctacgcga
tgcttcgcaagcgagctacgtgaacaacttcggtcctgggggcgtattcgagcaggatgacgcagaagcatggaaagcc
atcaccgaatccgtccagggaccgtttgccggtgaaggcctgcttaactatgaaatgggcatggatctcacccctctga
cagattggcccgggccgggtgaagctttgccgtctggttatgctgaacagaatcaaagacggttttggggccgatggct
ggaatacatgggccagcctccggcatttggaggcagggcatgatactagagaaagaggggacatactagatgaacgcgg
tggctgttgaccaggatactcgcgaagcggttgaggagttcatgttccgtgaagctgaactgctcgacggaggacaatt
ccgcgagtggctgggcttgttagaccctgatatcagatatgtcgtgcctgtgcggacgactcgcgaagactccgcagga
tgggtgggtgcaatcgcacactggaatgatgattacaccggcctggaaatgcgggttctgcgcggcgagaccgattttt
cgtgggctgaaagcccacgttctcgtactcgtcattttgttagtaatatccggactacagccggtccagaagctgatga
attaacggtgcgtagcaatcttctcttcttcaggtcgcgcggcgattcgggccgctgggaactgttatcagccgaacgt
gtggacgttcttcgccgcacggatgatagcctgcggcttgccagaagagaagttctcctagaccattctacccttccta
ttgacaatttatcagtgtttttatagtactagagaaagaggggacatactagagttttggatatggaaaaacaaaagaa
gaagaaatgtgaatagaaggttggataaagaagctaataaaaggggatgatattgaggaactggaaataggacataaag
aaggtgatggggtatagataagagttaatgtaagaggggggcatgttactagagaaagaggggacatactagagttttg
gatatggaaaaacaaaagaagaagaaatgtgaatagaaggttggataaagaagctaataaaaggggatgatattgagga
actggaaataggacataaagaaggtgatggggtatagataagagttaatgtaagaggggggcatgttactagagaaaga
ggggacatactagaggtgtccgatgcacaggtagccaagaccgacgtcgtgctggtcggtgcaggcatcatgagcgcca
cgctgagcgccctgatcaagctgctcgagcccaactggtcgatcaccctgatcgagcggctcgacggcgccgccgccga
gagcagcgacccctggaacaacgcaggcaccgggcactccgcgctgtgcgagctcaactacaccccggaggggcccggc
gggtcgatcgacatcaccaaagcggtgcacgtcaacgagcagttccaggtgtcgcgacagttctggacgtacgccgtgg
agaacggcgtgctgcccgatgtgcgcaacttcatcaacccgatcccgcacgtcagcttcgtcagcggtgcaaggaacgt
ggagtacctgcgcgcccgttacgacgcgttggtccccaacccgctgttcgcgacgatggagtacatcgacgaccgcgac
gagttcgcccggcggctgccgttcatggccgacaaacgcgatttccgcgagccggtcgcgctgaactggtcccaggacg
gcaccgacatcgatttcggctcgctgtcgcggcagctgatcggctacacggcccagcggggcatgacgacgctgttcgg
tcacgaggtccgcgacctggacaagcagtccgacggcagctggtcggtcaaggtggtcaaccgccgcaccggcgccaag
cgcaagctcaacgcgaagttcgtcttcgtcggcgcgggcggcggcgcgctgccgctgctccagaaggccgggatcgagg
aggccaagggcttcggcggcttcccggtgggcgggcagtggttgcgcaccggcaatcccgagctcaccgcgcgccacca
ggccaaggtgtacgggatgccgcccctgggcgcgccgccgatgtcggtgccccacctcgacacgcgcgtcatcaacggg
aagtcctggctgctgttcggtccgttcgcgggctggtcgccgaagttcctcaaacagggcaaggtcaccgatctgccgt
tctcggtcaagccgaacaacctggcgtcgatgctgggtgtcgggctgaccgagatgggcctgctcaagtacctgatcgg
ccagctgctactcagcgaggccgaccgggtggagacgctgcgcaacttcgcccccagcgcacgggactccgactgggag
ctcgacatcgccggtcagcgggtgcaggtgatccgccgcaagggcaagggcggcgtgctggagttcggcaccacggtcc
tggccgccaaggacggcagcatcgcaggcctgctcggggcgtcgccgggggcgtcgaccgccgtgcccgcgatgctcga
tgtcatggagcgttgcttcggcggccggtacaccgcctggctgccgaagctcaaggaaatcgtgccgtcgctgggcacc
aagctctccgacgagccgaagctgttccaggagatctgggcgcacgggacgaaggtgctcaagctggaccacccggccg
cggggctgccggtcgccggaaccgacaccgaacaccgtgagcccgcaaccacggtgaccgcctgatgatactagagaaa
gaggggacatactagatggcagcttctccacggtggcagtgcgaccgggcggcgggcgcgtccggggccattcagtttc
acggggtgattacggatgaagatggtcgaccagttcctaacgtcctgatcgagacctggcacgccgcgggggacgacga
caacgcggcgacgaagatcggacggccgcggtgcgacggccggaactacacccgcccaaagagcgtcatcaccgaccaa
cagggtcgttacagcatgagaatggtgccgccacgagcacccgagcacggggcagcgccgttcattgcggtgtcggtgc
atgcacgagggcttctcgatcggctgataacccgcgcctaccttccagggtgccatctcgccaaggaccccctgctccg
ccgcctgcccgccgagcggcggcaagccctgattgccacccgcgacgacatcggacttcgtttcgacatcacgctgcgg
ccagcacacggcgtgagggttgaaacctttgttgagccgcaacaggaagctgcgacgtgatactagagaaagaggggac
atactagaggtgacgcgtatcgcacccggcggattccgggaactgggacccctgaactgggccatcgcgaaggcgggtg
cccgtgccatccgcgcgccgcgcttcagcctgttcaacgtgctcggccaacaccgtctgctgttcctggcgttcctgcc
ctacagcggtgtgctgctcggccgcctcggcaagctgtcccgtcaggacgccgaactcgtgatcctgcgggtcggccat
ctgcgcgagtgtgagtacgaactccagcagcaccgacggctggcccgcagccgcggcgtcggcgcggaccttcaggccc
ggatcttcgagggccccgacgccgagggtctcaccgaccggcagcgggtgctcgtcaccgccaccgacgagttcgtcgt
cacccgaggggtgtcaccgcagacgtggtcggtgctggcccggcatctcaccaaacctcagctcatcgagttctgcctg
ctggcagcgcattacgacggtctggcggcgacgatcagcacgctccaggtgccgctcgacttcccggactgatgatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagtagcggccgct
gcag