All protein coding sequences come from Mycobacterium MKS strand
This is the Full composite part with Prefix/Suffix ready to clone into a plasmid.
Many restriction sites mutated from coding regions of DNA
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Full-length stationary phase osmY promoter
This part was PCRed out of E. coli's genome.
osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
Comes from the genome(s) of M. sp. KMS (as well as M. sp. JLS and M. sp. MCS).
Beta subunit of aromatic ring hydroxylating dioxygenase found in Mycobacterium sp. KMS. This protein is used in the pathway identified to degrade pyrene and other polycyclic aromatic hydrocarbons.
The amino acid sequence was used to back-translate into a DNA sequence more compatible with E. coli codon encoding through DNA 2.0.
malate--quinone oxidoreductase (Mkms_2115)
KEGG: mmc:Mmcs_2069 malate dehydrogenase (acceptor) TIGRFAM: malate--quinone oxidoreductase PFAM: FAD dependent oxidoreductase; Malate:quinone-oxidoreductase
639791342 intradiol ring-cleavage dioxygenase [Mycobacterium sp. KMS: NC_008705] (+)strand
PFAM: Carboxymuconolactone decarboxylase KEGG: mmc:Mmcs_0633 carboxymuconolactone decarboxylase
This is the alpha subunit of a dioxygenase protein found in Mycobacterium KMS. This protein is used to add hydroxyl groups to polycyclic aromatic hydrocarbons (PAHs).
Conversion of the iGEM parts registry to SBOL2.1