Promoter, RBS, and terminator parts came from the registry. The coding sequences came from genomic sequences found within the NCBI and JGI databases.
This composite part provides all the functions necessary to produce extracellular lignin degrading enzymes.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
Lignin Peroxidase gene from Mycobacterium marinum
This gene sequence will code for a lignin peroxidase which catalyzes the oxidation of phenolic and non-phenolic compounds and aromatic amines, aromatic ethers and polycyclic aromatic hydrocarbons.
Secretion tag may need to be incorporated for secretion and proper function.
Laccase gene from Streptomyces lavendulae
Laccase directly oxidizes phenolic subunits only, which comprises ~10% of the total polymer in natural lignin.
May need to add or change secretion tag to work properly with your host.
Conversion of the iGEM parts registry to SBOL2.1