LOCUS BBa_M11201 6219 bp DNA linear 22-Dec-2024 DEFINITION pRS316 plasmid for S. cerevisiae in BioBrick format ACCESSION BBa_M11201 VERSION BBa_M11201.1 FEATURES Location/Qualifiers misc_feature 3349..3354 /label=EcoRI misc_feature 3355..3360 /label=XbaI misc_feature 3373..3378 /label=SpeI misc_feature 3379..3384 /label=PstI CDS 523..1783 /label=Tryptophan Synthase misc_feature 2913..3486 /label=lacZ misc_feature 4645..5503 /label=Ampicillin Resistance ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 361 tcttaaccca actgcacaga acaaaaacct gtaggaaacg aagataaaaa atattttaat 421 ctatttctat atttctttta ttttatttta ttttatttat ttcattttat ttttgaagaa 481 aaaaaaacaa ttgaaactat ttttttttct tattttaaaa taatgacaga taatacttat 541 ttaaataatt tcccagattc aaatggttat tttggtaaat atggtggaaa atatgtacct 601 gaagtaatta ataatgcaat gaaagaaata gaagatgctt ataataaaat aagtaaaagt 661 gaagatttta ttaatgaatt aaaaaaaata agaaaagaat ttcaaggaag accaacacca 721 atatattatg caaaaaattt gacaaaaaaa tatggtggtg cagaaatata tttaaaaaga 781 gaagatttaa atcatactgg tgcacataaa ttaaatcatt gtatgggaga agcattatta 841 gcaaaatata tgggtaaaaa aaaattaata gcagaaacag gagcaggaca acatggtgta 901 gcattagcaa cagcagcagc atattttggt ttagaatgtg aaatacatat gggagaagta 961 gatgtaaaaa aagaatatcc aaatgtaatt agaatgaaaa tattaggtgc aaaagtagta 1021 tgtgtagaat ttggtgataa aacattaaaa gaagcagtag attcagcatt tgaagcatat 1081 ataaaagata tagataatac attttatgca ataggttcag tagtaggacc acatccattt 1141 ccaaaaatgg tacgtgattt tcaatcaatt gtaggtatag aatcaagaga acaattttta 1201 gaaatgaatg gtgatggtat attaccagat attgttacag catgtgtagg tggtggtagt 1261 aatgcaatgg gtatatttag tggttttata tcagataaac aagtagaatt aatttgtgtt 1321 gaaccattag gtaaaggaaa taaaataggt gaacatgcag caagtataac atatggtagt 1381 gaaggaataa tgcatggatt taattcaata atgttaaaag atgaagaagg aaatccatca 1441 aaagtacata gtatagcaag tggtttagat tatccaagtg taggtccaga aattgcatat 1501 ttaaatagta ttggtcgtac aaaaactgtt tgtattacag atcaagaagc aattaatgga 1561 ttttttgaat taagtagaac agagggtata ataccagcaa tagaatcaag tcatgcaatt 1621 ggatatgtat taaaaatagc aaaagaaatg aaaggaaaga aaattttaat aaatttgagt 1681 ggtagaggag ataaagattt agattttgtt gtacaaaatt atggatatgg taataattaa 1741 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca 1801 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta 1861 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa 1921 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat 1981 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata 2041 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga 2101 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag 2161 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat 2221 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta 2281 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta 2341 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg 2401 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa 2461 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt 2521 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa 2581 actcacaaat tagagcttca atttaattat atcagttatt accctgcggt gtgaaatacc 2641 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta 2701 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 2761 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 2821 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 2881 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 2941 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 3001 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 3061 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 3121 cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 3181 cgggcctctt cgctattacg ccagctggcg aaggggggat gtgctgcaag gcgattaagt 3241 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa 3301 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgcga attctctaga 3361 ggatcccccg ggactagtct gcaggatatc aagcttatcg ataccgtcga cctcgagggg 3421 gggcccggta cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc 3481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg 3541 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat 3601 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3661 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 3721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctcgg 3901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3961 actataaaga taccaggcgt tcccccctgg aagctccctc gtgcgctctc ctgttccgac 4021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 4081 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4321 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4561 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4681 gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat 4741 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 4801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 4861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4921 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 4981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 5041 atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 5101 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 5161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 5221 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 5281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 5341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 5401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 5461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 5521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc 5641 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa 5701 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg 5761 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc 5821 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg 5881 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt 5941 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt 6001 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca 6061 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac 6121 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat 6181 taacctataa aaataggcgt atcacgaggc cctttcgtc //