LOCUS       BBa_M11205              2691 bp    DNA     linear       22-Nov-2024
DEFINITION  HXT1 promoter + GFP to monitor glucose levels in S. cerevisiae
ACCESSION   BBa_M11205
VERSION     BBa_M11205.1  
FEATURES             Location/Qualifiers
     promoter        1..1713
                     /label=HXT1
     promoter        1..1713
                     /label=BBa_M11202
     RBS             1722..1739
                     /label=BBa_K165002
     CDS             1746..2458
                     /label=yeast GFP CDS
     CDS             1746..2458
                     /label=BBa_K194001
     misc_feature    2467..2691
                     /label=ADH1 terminator
     terminator      2467..2691
                     /label=BBa_J63002
ORIGIN
        1 atgaactcaa ctcccgatct aatatctcct cagaaatcca attcatccaa ctcatatgaa
       61 ttggaatctg gtcgttcaaa ggccatgaat actccagaag gtaaaaatga aagttttcac
      121 gacaacttaa gtgaaagtca agtgcaaccc gccgttgccc ctccaaacac cggaaaaggt
      181 gtctacgtaa cggtttctat ctgttgtgtt atggttgctt tcggtggttt catatttgga
      241 tgggatactg gtaccatttc tggttttgtt gctcaaactg attttctaag aagatttggt
      301 atgaagcacc acgacggtag tcattacttg tccaaggtga gaactggttt aattgtctct
      361 atttttaaca ttggttgtgc cattggtggt atcgtcttag ccaagctagg tgatatgtat
      421 ggtcgtagaa tcggtttgat tgtcgttgta gtaatctaca ctatcggtat cattattcaa
      481 atagcctcga tcaacaagtg gtaccaatat ttcattggta gaattatctc tggtttaggt
      541 gtcggtggta tcacagtttt atctcccatg ctaatatctg aggtcgcccc cagtgaaatg
      601 agaggcacct tggtttcatg ttaccaagtc atgattactt taggtatttt cttaggttac
      661 tgtaccaatt ttggtaccaa gaattactca aactctgtcc aatggagagt tccattaggt
      721 ttgtgtttcg cctgggcctt atttatgatt ggtggtatga tgtttgttcc tgaatctcca
      781 cgttatttgg ttgaagctgg cagaatcgac gaagccaggg cttctttagc taaagttaac
      841 aaatgcccac ctgaccatcc atacattcaa tatgagttgg aaactatcga agccagtgtc
      901 gaagaaatga gagccgctgg tactgcatct tggggcgaat tattcactgg taaaccagcc
      961 atgtttcaac gtactatgat gggtatcatg attcaatctc tacaacaatt aactggtgat
     1021 aactatttct tctactacgg taccattgtt ttccaggctg tcggtttaag tgactctttt
     1081 gaaacttcta ttgtctttgg tgtcgtcaac ttcttctcca cttgttgttc tctgtacacc
     1141 gttgaccgtt ttggccgtcg taactgtttg atgtggggtg ctgtcggtat ggtctgctgt
     1201 tatgttgtct atgcctctgt tggtgttacc agattatggc caaacggtca agatcaacca
     1261 tcttcaaagg gtgctggtaa ctgtatgatt gttttcgcat gtttctacat tttctgtttc
     1321 gctactacct gggccccaat tgcttacgtt gttatttcag aatgtttccc attaagagtc
     1381 aaatccaagt gtatgtctat tgccagtgct gctaactgga tctggggttt cttgattagt
     1441 ttcttcaccc catttattac tggtgccatc aacttctact acggttacgt tttcatgggc
     1501 tgtatggttt tcgcttactt ttacgtcttt ttcttcgttc cagaaactaa aggtttatca
     1561 ttagaagaag ttaatgatat gtacgccgaa ggtgttctac catggaaatc agcttcctgg
     1621 gttccagtat ccaagagagg cgctgactac aacgctgatg acctaatgca tgatgaccaa
     1681 ccattttaca agagtttgtt tagcaggaaa taatactaga gcccgccgcc accatggagt
     1741 actagatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag
     1801 atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt
     1861 acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa
     1921 ccttagtcac tactttcggt tatggtgttc aatgttttgc gagataccca gatcatatga
     1981 aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt
     2041 ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct
     2101 tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc
     2161 acaaattgga atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga
     2221 atggtatcaa agttaacttc aaaattagac acaacattga agatggttct gttcaattag
     2281 ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc
     2341 attacttatc cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg
     2401 tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa tttaataata
     2461 ctagagtaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     2521 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     2581 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt
     2641 attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc c
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