LOCUS BBa_M11207 3759 bp DNA linear 23-Dec-2024 DEFINITION Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae ACCESSION BBa_M11207 VERSION BBa_M11207.1 FEATURES Location/Qualifiers promoter 1..1713 /label=HXT7 promoter 1..1713 /label=BBa_M11203 RBS 1722..1739 /label=BBa_K165002 CDS 1746..2458 /label=yeast GFP CDS CDS 1746..2458 /label=BBa_K194001 misc_feature 2467..2691 /label=ADH1 terminator terminator 2467..2691 /label=BBa_J63002 misc_feature 1..2691 /label=BBa_M11204 promoter 2775..2782 /label=-10 promoter 2747..2755 /label=-35 misc_feature 2741..2754 /label=Fnr misc_feature 2737..2755 /label=Fis misc_feature 2703..2716 /label=Fnr misc_feature 2719..2725 /label=NarL misc_feature 2706..2712 /label=NarL promoter 2700..2788 /label=BBa_K239006 RBS 2797..2814 /label=BBa_K165002 CDS 2821..3495 /label=mrfp1 misc_feature 3502..3526 /label=Help:Barcodes CDS 2821..3526 /label=BBa_E1010 misc_feature 3535..3759 /label=ADH1 terminator terminator 3535..3759 /label=BBa_J63002 misc_feature 2700..3759 /label=BBa_M11206 ORIGIN 1 atgtcacaag acgctgctat tgcagagcaa actcctgtgg agcatctctc tgctgttgac 61 tcagcctccc actcggtttt atctacacca tcaaacaagg ctgaaagaga tgaaataaaa 121 gcttatggtg aaggtgaaga gcacgaacct gtcgttgaaa ttccaaagag accagcttct 181 gcctatgtca ctgtctctat tatgtgtatc atgatcgcct ttggtggttt cgttttcggt 241 tgggatactg gtaccatttc tggtttcatc aatcaaaccg atttcatcag aagatttggt 301 atgaagcata aagatggtac taattatttg tctaaggtta gaactggttt gattgtctcc 361 attttcaaca ttggttgtgc cattggtggt attattcttt ccaaattggg tgatatgtac 421 ggtcgtaagg tgggtttgat tgtcgttgtt gtcatctaca tcatcggtat tattattcaa 481 attgcatcta tcaacaaatg gtaccaatat ttcatcggta gaattatttc cggtttgggt 541 gttggtggta ttgccgtttt atctcctatg ttgatttctg aagtatcccc aaagcattta 601 aggggtactt tagtctcttg ctaccaattg atgattactg ccggtatttt cttgggttac 661 tgtaccaact tcggtactaa gaactactcc aactctgtgc aatggagagt tccattaggt 721 ttgtgttttg cctgggcttt gtttatgatt ggtggtatga catttgttcc agagtctcca 781 cgttatttgg ctgaagtcgg taagatcgaa gaagccaaac gttctattgc cgtttctaac 841 aaggttgctg ttgatgatcc atctgttttg gctgaagtcg aagctgtctt ggctggtgta 901 gaggcagaga aattagctgg taatgcatcc tggggtgaat tgtttagtag caagacaaag 961 gtccttcagc gtttgatcat gggtgctatg attcaatctc tacaacaatt gacaggtgat 1021 aactatttct tctactatgg tactactatt ttcaaggctg ttggtttgag tgactctttc 1081 gaaacctcta ttgtcttggg tattgttaac tttgcttcca cctttgttgg tatttacgtt 1141 gttgagagat atggtcgtcg tacttgtttg ctatggggtg ctgcatccat gactgcttgt 1201 atggttgtct atgcttccgt gggtgtcacc agattatggc caaatggtca agaccaacca 1261 tcttccaagg gtgctggtaa ctgtatgatt gtctttgcct gtttctatat tttctgtttt 1321 gctactacat gggctccaat tccttatgtc gttgtttctg aaactttccc attgagagtc 1381 aagtctaagg ctatgtctat tgctacagct gctaattggt tgtggggttt cttgattggt 1441 ttcttcactc catttattac tggtgctatt aacttctact acggttacgt tttcatgggc 1501 tgtttggtct tcatgttctt ctatgttttg ttagttgttc cagaaactaa gggtttgact 1561 ttggaagaag tcaacaccat gtgggaagaa ggtgttctac catggaagtc tgcctcatgg 1621 gttccaccat ccagaagagg tgccaactac gacgctgaag aaatgactca cgatgacaag 1681 ccattgtaca agagaatgtt cagcaccaaa taatactaga gcccgccgcc accatggagt 1741 actagatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag 1801 atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt 1861 acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa 1921 ccttagtcac tactttcggt tatggtgttc aatgttttgc gagataccca gatcatatga 1981 aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt 2041 ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct 2101 tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc 2161 acaaattgga atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga 2221 atggtatcaa agttaacttc aaaattagac acaacattga agatggttct gttcaattag 2281 ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc 2341 attacttatc cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg 2401 tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa tttaataata 2461 ctagagtaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa 2521 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc 2581 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt 2641 attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ctactagagg 2701 tattgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc 2761 tttagctaca gacactaagg tggcagacta ctagagcccg ccgccaccat ggagtactag 2821 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt 2881 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt 2941 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc 3001 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg 3061 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa 3121 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac 3181 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc 3241 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa 3301 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc 3361 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac 3421 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt 3481 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagtaataa 3541 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta 3601 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct 3661 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc 3721 tctaccggca tgccgagcaa atgcctgcaa atcgctccc //