Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae
Composite of the following BioBrick devices: BBa_M11204, BBa_M11206
Used in S. cerevisiae to monitor glucose and oxygen levels. It is composed of two BioBrick devices: Device 1: The promoter, HXT7, is repressed in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae. Device 2: The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
HXT7 promoter + GFP to monitor glucose levels in S. cerevisiae
Composite of the following BioBrick parts: BBa_M11203 + BBa_K165002+ BBa_K194001+ BBa_J63002
Used in S. cerevisiae to monitor glucose levels. The promoter, HXT7, is repressed in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
mNarK promoter + RFP to monitor oxygen monitors in S. cerevisiae
Composite of the following parts: BBa_K239006, BBa_K165002, BBa_E1010, BBa_J63002
Used in S. cerevisiae to monitor oxygen levels. The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
HXT7 promoter for S. cerevisiae, repressed in the presence of glucose
Modified from the genomic sequence of HXT7 in S. cerevisiae at nucleotide location site: 1154210???1155922
Repressed in presence of glucose 570 aa Found in S. cerevisiae Nucleotide Location: 1154210???1155922
Added standard BioBrick prefix and suffix
Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.
Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.
GFP (a yeast- and FACS-optimized GFP variant),
Cormack BP, Bertram G, Egerton M, Gow NAR, Falkow S, Brown AJP. 1997. Yeast-enhanced green ??uorescent protein (yEGFP): a reporter of gene expression in Candida albicans. Microbiology 143: 303??311.
Green Fluorescent Protein (GFP) codonoptimized for yeast.
the biobrick follow the biobrick standards
Conversion of the iGEM parts registry to SBOL2.1