LOCUS BBa_M11208 3759 bp DNA linear 27-Dec-2024 DEFINITION Device containing HXT1/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae ACCESSION BBa_M11208 VERSION BBa_M11208.1 FEATURES Location/Qualifiers promoter 1..1713 /label=HXT1 promoter 1..1713 /label=BBa_M11202 RBS 1722..1739 /label=BBa_K165002 CDS 1746..2458 /label=yeast GFP CDS CDS 1746..2458 /label=BBa_K194001 misc_feature 2467..2691 /label=ADH1 terminator terminator 2467..2691 /label=BBa_J63002 misc_feature 1..2691 /label=BBa_M11205 promoter 2775..2782 /label=-10 promoter 2747..2755 /label=-35 misc_feature 2741..2754 /label=Fnr misc_feature 2737..2755 /label=Fis misc_feature 2703..2716 /label=Fnr misc_feature 2719..2725 /label=NarL misc_feature 2706..2712 /label=NarL promoter 2700..2788 /label=BBa_K239006 RBS 2797..2814 /label=BBa_K165002 CDS 2821..3495 /label=mrfp1 misc_feature 3502..3526 /label=Help:Barcodes CDS 2821..3526 /label=BBa_E1010 misc_feature 3535..3759 /label=ADH1 terminator terminator 3535..3759 /label=BBa_J63002 misc_feature 2700..3759 /label=BBa_M11206 ORIGIN 1 atgaactcaa ctcccgatct aatatctcct cagaaatcca attcatccaa ctcatatgaa 61 ttggaatctg gtcgttcaaa ggccatgaat actccagaag gtaaaaatga aagttttcac 121 gacaacttaa gtgaaagtca agtgcaaccc gccgttgccc ctccaaacac cggaaaaggt 181 gtctacgtaa cggtttctat ctgttgtgtt atggttgctt tcggtggttt catatttgga 241 tgggatactg gtaccatttc tggttttgtt gctcaaactg attttctaag aagatttggt 301 atgaagcacc acgacggtag tcattacttg tccaaggtga gaactggttt aattgtctct 361 atttttaaca ttggttgtgc cattggtggt atcgtcttag ccaagctagg tgatatgtat 421 ggtcgtagaa tcggtttgat tgtcgttgta gtaatctaca ctatcggtat cattattcaa 481 atagcctcga tcaacaagtg gtaccaatat ttcattggta gaattatctc tggtttaggt 541 gtcggtggta tcacagtttt atctcccatg ctaatatctg aggtcgcccc cagtgaaatg 601 agaggcacct tggtttcatg ttaccaagtc atgattactt taggtatttt cttaggttac 661 tgtaccaatt ttggtaccaa gaattactca aactctgtcc aatggagagt tccattaggt 721 ttgtgtttcg cctgggcctt atttatgatt ggtggtatga tgtttgttcc tgaatctcca 781 cgttatttgg ttgaagctgg cagaatcgac gaagccaggg cttctttagc taaagttaac 841 aaatgcccac ctgaccatcc atacattcaa tatgagttgg aaactatcga agccagtgtc 901 gaagaaatga gagccgctgg tactgcatct tggggcgaat tattcactgg taaaccagcc 961 atgtttcaac gtactatgat gggtatcatg attcaatctc tacaacaatt aactggtgat 1021 aactatttct tctactacgg taccattgtt ttccaggctg tcggtttaag tgactctttt 1081 gaaacttcta ttgtctttgg tgtcgtcaac ttcttctcca cttgttgttc tctgtacacc 1141 gttgaccgtt ttggccgtcg taactgtttg atgtggggtg ctgtcggtat ggtctgctgt 1201 tatgttgtct atgcctctgt tggtgttacc agattatggc caaacggtca agatcaacca 1261 tcttcaaagg gtgctggtaa ctgtatgatt gttttcgcat gtttctacat tttctgtttc 1321 gctactacct gggccccaat tgcttacgtt gttatttcag aatgtttccc attaagagtc 1381 aaatccaagt gtatgtctat tgccagtgct gctaactgga tctggggttt cttgattagt 1441 ttcttcaccc catttattac tggtgccatc aacttctact acggttacgt tttcatgggc 1501 tgtatggttt tcgcttactt ttacgtcttt ttcttcgttc cagaaactaa aggtttatca 1561 ttagaagaag ttaatgatat gtacgccgaa ggtgttctac catggaaatc agcttcctgg 1621 gttccagtat ccaagagagg cgctgactac aacgctgatg acctaatgca tgatgaccaa 1681 ccattttaca agagtttgtt tagcaggaaa taatactaga gcccgccgcc accatggagt 1741 actagatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag 1801 atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt 1861 acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa 1921 ccttagtcac tactttcggt tatggtgttc aatgttttgc gagataccca gatcatatga 1981 aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt 2041 ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct 2101 tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc 2161 acaaattgga atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga 2221 atggtatcaa agttaacttc aaaattagac acaacattga agatggttct gttcaattag 2281 ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc 2341 attacttatc cactcaatct gccttatcca aagatccaaa cgaaaagaga gaccacatgg 2401 tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa tttaataata 2461 ctagagtaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa 2521 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc 2581 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt 2641 attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ctactagagg 2701 tattgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc 2761 tttagctaca gacactaagg tggcagacta ctagagcccg ccgccaccat ggagtactag 2821 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt 2881 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt 2941 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc 3001 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg 3061 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa 3121 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac 3181 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc 3241 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa 3301 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc 3361 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac 3421 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt 3481 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagtaataa 3541 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta 3601 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct 3661 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc 3721 tctaccggca tgccgagcaa atgcctgcaa atcgctccc //