BBa_M11208
type: ComponentDefinition
persistent identity: BBa M11208
display id: BBa_M11208
version: 1
was generated by: iGEM to SBOL conversion
description: Device containing HXT1/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae
created: 2010-04-21T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: Composite of the following BioBrick devices: BBa_M11205, BBa_M11206
owned by: james
owned by: myers
mutable description: Used in S. cerevisiae to monitor glucose and oxygen levels. It is composed of two BioBrick devices: Device 1: The promoter, HXT1, is activated in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae. Device 2: The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
discontinued: false
dominant: false
group u list: _572_
m user id: 0
owner id: 6793
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Angela Dixon
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_M11206
component: BBa_M11205
sequence annotation: BBa_M11205
sequence annotation: BBa_M11206
sequence: https://synbiohub.org/public/igem/BBa_M11208_sequence/1

BBa_M11206
type: Component
persistent identity: component2272596
display id: component2272596
version: 1
top level: BBa_M11208
definition: BBa_M11206
access: public

BBa_M11205
type: Component
persistent identity: component2272581
display id: component2272581
version: 1
top level: BBa_M11208
definition: BBa_M11205
access: public

BBa_M11205
type: SequenceAnnotation
persistent identity: annotation2272581
display id: annotation2272581
version: 1
top level: BBa_M11208
location: https://synbiohub.org/public/igem/BBa_M11208/annotation2272581/range2272581/1
component: BBa_M11205

https://synbiohub.org/public/igem/BBa_M11208/annotation2272581/range2272581/1
type: Range
persistent identity: range2272581
display id: range2272581
version: 1
top level: BBa_M11208
direction: direction/reverse
start: 1
end: 2691
orientation: inline

BBa_M11206
type: SequenceAnnotation
persistent identity: annotation2272596
display id: annotation2272596
version: 1
top level: BBa_M11208
location: https://synbiohub.org/public/igem/BBa_M11208/annotation2272596/range2272596/1
component: BBa_M11206

https://synbiohub.org/public/igem/BBa_M11208/annotation2272596/range2272596/1
type: Range
persistent identity: range2272596
display id: range2272596
version: 1
top level: BBa_M11208
direction: direction/reverse
start: 2700
end: 3759
orientation: inline

BBa_M11205
type: ComponentDefinition
persistent identity: BBa M11205
display id: BBa_M11205
version: 1
was generated by: iGEM to SBOL conversion
description: HXT1 promoter + GFP to monitor glucose levels in S. cerevisiae
created: 2010-04-21T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: Composite of the following parts: BBa_M11202, BBa_K165002, BBa_K194001, BBa_J63002
owned by: james
owned by: myers
mutable description: Used in S. cerevisiae to monitor glucose levels. The promoter, HXT1, is activated in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
discontinued: false
dominant: false
group u list: _572_
m user id: 0
owner id: 6793
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Angela Dixon
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J63002
component: BBa_K194001
component: BBa_K165002
component: BBa_M11202
sequence annotation: BBa_M11202
sequence annotation: BBa_K165002
sequence annotation: BBa_K194001
sequence annotation: BBa_J63002
sequence: https://synbiohub.org/public/igem/BBa_M11205_sequence/1

BBa_J63002
type: Component
persistent identity: component2220618
display id: component2220618
version: 1
top level: BBa_M11205
definition: tADH1
access: public

BBa_K194001
type: Component
persistent identity: component2220616
display id: component2220616
version: 1
top level: BBa_M11205
definition: BBa_K194001
access: public

BBa_K165002
type: Component
persistent identity: component2220614
display id: component2220614
version: 1
top level: BBa_M11205
definition: BBa_K165002
access: public

BBa_M11202
type: Component
persistent identity: component2220613
display id: component2220613
version: 1
top level: BBa_M11205
definition: BBa_M11202
access: public

BBa_M11202
type: SequenceAnnotation
persistent identity: annotation2220613
display id: annotation2220613
version: 1
top level: BBa_M11205
location: https://synbiohub.org/public/igem/BBa_M11205/annotation2220613/range2220613/1
component: BBa_M11202

https://synbiohub.org/public/igem/BBa_M11205/annotation2220613/range2220613/1
type: Range
persistent identity: range2220613
display id: range2220613
version: 1
top level: BBa_M11205
direction: direction/reverse
start: 1
end: 1713
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation2220614
display id: annotation2220614
version: 1
top level: BBa_M11205
location: https://synbiohub.org/public/igem/BBa_M11205/annotation2220614/range2220614/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_M11205/annotation2220614/range2220614/1
type: Range
persistent identity: range2220614
display id: range2220614
version: 1
top level: BBa_M11205
direction: direction/reverse
start: 1722
end: 1739
orientation: inline

BBa_K194001
type: SequenceAnnotation
persistent identity: annotation2220616
display id: annotation2220616
version: 1
top level: BBa_M11205
location: https://synbiohub.org/public/igem/BBa_M11205/annotation2220616/range2220616/1
component: BBa_K194001

https://synbiohub.org/public/igem/BBa_M11205/annotation2220616/range2220616/1
type: Range
persistent identity: range2220616
display id: range2220616
version: 1
top level: BBa_M11205
direction: direction/reverse
start: 1746
end: 2458
orientation: inline

BBa_J63002
type: SequenceAnnotation
persistent identity: annotation2220618
display id: annotation2220618
version: 1
top level: BBa_M11205
location: https://synbiohub.org/public/igem/BBa_M11205/annotation2220618/range2220618/1
component: BBa_J63002

https://synbiohub.org/public/igem/BBa_M11205/annotation2220618/range2220618/1
type: Range
persistent identity: range2220618
display id: range2220618
version: 1
top level: BBa_M11205
direction: direction/reverse
start: 2467
end: 2691
orientation: inline

BBa_M11206
type: ComponentDefinition
persistent identity: BBa M11206
display id: BBa_M11206
version: 1
was generated by: iGEM to SBOL conversion
description: mNarK promoter + RFP to monitor oxygen monitors in S. cerevisiae
created: 2010-04-21T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: Composite of the following parts: BBa_K239006, BBa_K165002, BBa_E1010, BBa_J63002
owned by: james
owned by: myers
mutable description: Used in S. cerevisiae to monitor oxygen levels. The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.
discontinued: false
dominant: false
group u list: _572_
m user id: 0
owner id: 6793
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Angela Dixon
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K165002
component: BBa_K239006
component: BBa_J63002
component: BBa_E1010
sequence annotation: BBa_K239006
sequence annotation: BBa_K165002
sequence annotation: BBa_E1010
sequence annotation: BBa_J63002
sequence: https://synbiohub.org/public/igem/BBa_M11206_sequence/1

BBa_K165002
type: Component
persistent identity: component2250133
display id: component2250133
version: 1
top level: BBa_M11206
definition: BBa_K165002
access: public

BBa_K239006
type: Component
persistent identity: component2250132
display id: component2250132
version: 1
top level: BBa_M11206
definition: BBa_K239006
access: public

BBa_J63002
type: Component
persistent identity: component2250138
display id: component2250138
version: 1
top level: BBa_M11206
definition: tADH1
access: public

BBa_E1010
type: Component
persistent identity: component2250136
display id: component2250136
version: 1
top level: BBa_M11206
definition: mRFP1
access: public

BBa_K239006
type: SequenceAnnotation
persistent identity: annotation2250132
display id: annotation2250132
version: 1
top level: BBa_M11206
location: https://synbiohub.org/public/igem/BBa_M11206/annotation2250132/range2250132/1
component: BBa_K239006

https://synbiohub.org/public/igem/BBa_M11206/annotation2250132/range2250132/1
type: Range
persistent identity: range2250132
display id: range2250132
version: 1
top level: BBa_M11206
direction: direction/reverse
start: 1
end: 89
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation2250133
display id: annotation2250133
version: 1
top level: BBa_M11206
location: https://synbiohub.org/public/igem/BBa_M11206/annotation2250133/range2250133/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_M11206/annotation2250133/range2250133/1
type: Range
persistent identity: range2250133
display id: range2250133
version: 1
top level: BBa_M11206
direction: direction/reverse
start: 98
end: 115
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250136
display id: annotation2250136
version: 1
top level: BBa_M11206
location: https://synbiohub.org/public/igem/BBa_M11206/annotation2250136/range2250136/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M11206/annotation2250136/range2250136/1
type: Range
persistent identity: range2250136
display id: range2250136
version: 1
top level: BBa_M11206
direction: direction/reverse
start: 122
end: 827
orientation: inline

BBa_J63002
type: SequenceAnnotation
persistent identity: annotation2250138
display id: annotation2250138
version: 1
top level: BBa_M11206
location: https://synbiohub.org/public/igem/BBa_M11206/annotation2250138/range2250138/1
component: BBa_J63002

https://synbiohub.org/public/igem/BBa_M11206/annotation2250138/range2250138/1
type: Range
persistent identity: range2250138
display id: range2250138
version: 1
top level: BBa_M11206
direction: direction/reverse
start: 836
end: 1060
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_K165002
type: ComponentDefinition
persistent identity: BBa K165002
display id: BBa_K165002
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak sequence (yeast RBS)
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K165002_sequence/1

BBa_M11202
type: ComponentDefinition
persistent identity: BBa M11202
display id: BBa_M11202
version: 1
was generated by: iGEM to SBOL conversion
description: HXT1 promoter for S. cerevisiae (activated by glucose)
created: 2010-04-20T11:00:00Z
modified: 2015-05-08T01:13:53Z
mutable provenance: Comes from the HXT1 genomic sequence in S. cerevisiae, genomic nucleotide location: 198705???200417
owned by: james
owned by: myers
mutable description: HXT1 promoter modified for BioBrick standard. HXT1 is found in S. cerevisiae and is activated in the presence of glucose.
discontinued: false
dominant: false
group u list: _572_
m user id: 0
owner id: 6793
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Used mutagenesis to remove EcoRI site at location 3...8. Added standard prefix and suffix for BioBrick.
star: false
creator: Angela Dixon
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: HXT1
sequence: https://synbiohub.org/public/igem/BBa_M11202_sequence/1

HXT1
type: SequenceAnnotation
persistent identity: annotation2068420
display id: annotation2068420
version: 1
top level: BBa_M11202
location: https://synbiohub.org/public/igem/BBa_M11202/annotation2068420/range2068420/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M11202/annotation2068420/range2068420/1
type: Range
persistent identity: range2068420
display id: range2068420
version: 1
top level: BBa_M11202
direction: direction/forward
start: 1
end: 1713
orientation: inline

BBa_K239006
type: ComponentDefinition
persistent identity: BBa K239006
display id: BBa_K239006
version: 1
was generated by: iGEM to SBOL conversion
description: Modified NarK promoter
created: 2009-06-25T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.
owned by: james
owned by: myers
mutable description: Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.
discontinued: false
dominant: false
group u list: _375_
m user id: 0
owner id: 4247
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.
star: false
creator: Axel Nystrom
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Fnr
sequence annotation: NarL
sequence annotation: Fis
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K239006_sequence/1

Fnr
type: SequenceAnnotation
persistent identity: annotation2006724
display id: annotation2006724
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006724/range2006724/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K239006/annotation2006724/range2006724/1
type: Range
persistent identity: range2006724
display id: range2006724
version: 1
top level: BBa_K239006
direction: direction/forward
start: 4
end: 17
orientation: inline

NarL
type: SequenceAnnotation
persistent identity: annotation2006726
display id: annotation2006726
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006726/range2006726/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K239006/annotation2006726/range2006726/1
type: Range
persistent identity: range2006726
display id: range2006726
version: 1
top level: BBa_K239006
direction: direction/forward
start: 7
end: 13
orientation: inline

NarL
type: SequenceAnnotation
persistent identity: annotation2006725
display id: annotation2006725
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006725/range2006725/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K239006/annotation2006725/range2006725/1
type: Range
persistent identity: range2006725
display id: range2006725
version: 1
top level: BBa_K239006
direction: direction/forward
start: 20
end: 26
orientation: inline

Fis
type: SequenceAnnotation
persistent identity: annotation2006723
display id: annotation2006723
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006723/range2006723/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K239006/annotation2006723/range2006723/1
type: Range
persistent identity: range2006723
display id: range2006723
version: 1
top level: BBa_K239006
direction: direction/forward
start: 38
end: 56
orientation: inline

Fnr
type: SequenceAnnotation
persistent identity: annotation2006722
display id: annotation2006722
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006722/range2006722/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K239006/annotation2006722/range2006722/1
type: Range
persistent identity: range2006722
display id: range2006722
version: 1
top level: BBa_K239006
direction: direction/forward
start: 42
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2006721
display id: annotation2006721
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006721/range2006721/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K239006/annotation2006721/range2006721/1
type: Range
persistent identity: range2006721
display id: range2006721
version: 1
top level: BBa_K239006
direction: direction/forward
start: 48
end: 56
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2006720
display id: annotation2006720
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006720/range2006720/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K239006/annotation2006720/range2006720/1
type: Range
persistent identity: range2006720
display id: range2006720
version: 1
top level: BBa_K239006
direction: direction/forward
start: 76
end: 83
orientation: inline

BBa_K194001
type: ComponentDefinition
persistent identity: BBa K194001
display id: BBa_K194001
version: 1
was generated by: iGEM to SBOL conversion
description: GFP (a yeast- and FACS-optimized GFP variant),
created: 2009-07-12T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: Cormack BP, Bertram G, Egerton M, Gow NAR, Falkow S, Brown AJP. 1997. Yeast-enhanced green ??uorescent protein (yEGFP): a reporter of gene expression in Candida albicans. Microbiology 143: 303??311.
owned by: james
owned by: myers
mutable description: Green Fluorescent Protein (GFP) codonoptimized for yeast.
discontinued: false
dominant: false
experience: experience/Works
group u list: _289_
m user id: 0
owner id: 3946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: the biobrick follow the biobrick standards
star: false
creator: Christian Kaas
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: yeast GFP CDS
sequence: https://synbiohub.org/public/igem/BBa_K194001_sequence/1

yeast GFP CDS
type: SequenceAnnotation
persistent identity: annotation2011260
display id: annotation2011260
version: 1
top level: BBa_K194001
location: https://synbiohub.org/public/igem/BBa_K194001/annotation2011260/range2011260/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K194001/annotation2011260/range2011260/1
type: Range
persistent identity: range2011260
display id: range2011260
version: 1
top level: BBa_K194001
direction: direction/forward
start: 1
end: 713
orientation: inline

tADH1
type: ComponentDefinition
persistent identity: BBa J63002
display id: BBa_J63002
version: 1
was generated by: iGEM to SBOL conversion
description: ADH1 terminator from S. cerevisiae
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: genomic DNA of S. cerevisiae
owned by: james
owned by: myers
mutable description: ADH1 terminator from S. cerevisiae
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: starts with stop codon
star: false
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1 terminator
sequence: https://synbiohub.org/public/igem/BBa_J63002_sequence/1

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation1902851
display id: annotation1902851
version: 1
top level: tADH1
location: https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1
type: Range
persistent identity: range1902851
display id: range1902851
version: 1
top level: tADH1
direction: direction/forward
start: 1
end: 225
orientation: inline

https://synbiohub.org/public/igem/BBa_M11208_sequence/1
type: Sequence
persistent identity: BBa M11208 sequence
display id: BBa_M11208_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaactcaactcccgatctaatatctcctcagaaatccaattcatccaactcatatgaattggaatctggtcgttcaaaggccatgaatactccagaaggtaaaaatgaaagttttcacgacaacttaagtgaaagtcaagtgcaacccgccgttgcccctccaaacaccggaaaaggtgtctacgtaacggtttctatctgttgtgttatggttgctttcggtggtttcatatttggatgggatactggtaccatttctggttttgttgctcaaactgattttctaagaagatttggtatgaagcaccacgacggtagtcattacttgtccaaggtgagaactggtttaattgtctctatttttaacattggttgtgccattggtggtatcgtcttagccaagctaggtgatatgtatggtcgtagaatcggtttgattgtcgttgtagtaatctacactatcggtatcattattcaaatagcctcgatcaacaagtggtaccaatatttcattggtagaattatctctggtttaggtgtcggtggtatcacagttttatctcccatgctaatatctgaggtcgcccccagtgaaatgagaggcaccttggtttcatgttaccaagtcatgattactttaggtattttcttaggttactgtaccaattttggtaccaagaattactcaaactctgtccaatggagagttccattaggtttgtgtttcgcctgggccttatttatgattggtggtatgatgtttgttcctgaatctccacgttatttggttgaagctggcagaatcgacgaagccagggcttctttagctaaagttaacaaatgcccacctgaccatccatacattcaatatgagttggaaactatcgaagccagtgtcgaagaaatgagagccgctggtactgcatcttggggcgaattattcactggtaaaccagccatgtttcaacgtactatgatgggtatcatgattcaatctctacaacaattaactggtgataactatttcttctactacggtaccattgttttccaggctgtcggtttaagtgactcttttgaaacttctattgtctttggtgtcgtcaacttcttctccacttgttgttctctgtacaccgttgaccgttttggccgtcgtaactgtttgatgtggggtgctgtcggtatggtctgctgttatgttgtctatgcctctgttggtgttaccagattatggccaaacggtcaagatcaaccatcttcaaagggtgctggtaactgtatgattgttttcgcatgtttctacattttctgtttcgctactacctgggccccaattgcttacgttgttatttcagaatgtttcccattaagagtcaaatccaagtgtatgtctattgccagtgctgctaactggatctggggtttcttgattagtttcttcaccccatttattactggtgccatcaacttctactacggttacgttttcatgggctgtatggttttcgcttacttttacgtctttttcttcgttccagaaactaaaggtttatcattagaagaagttaatgatatgtacgccgaaggtgttctaccatggaaatcagcttcctgggttccagtatccaagagaggcgctgactacaacgctgatgacctaatgcatgatgaccaaccattttacaagagtttgtttagcaggaaataatactagagcccgccgccaccatggagtactagatgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgcgagatacccagatcatatgaaacaacatgactttttcaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M11205_sequence/1
type: Sequence
persistent identity: BBa M11205 sequence
display id: BBa_M11205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaactcaactcccgatctaatatctcctcagaaatccaattcatccaactcatatgaattggaatctggtcgttcaaaggccatgaatactccagaaggtaaaaatgaaagttttcacgacaacttaagtgaaagtcaagtgcaacccgccgttgcccctccaaacaccggaaaaggtgtctacgtaacggtttctatctgttgtgttatggttgctttcggtggtttcatatttggatgggatactggtaccatttctggttttgttgctcaaactgattttctaagaagatttggtatgaagcaccacgacggtagtcattacttgtccaaggtgagaactggtttaattgtctctatttttaacattggttgtgccattggtggtatcgtcttagccaagctaggtgatatgtatggtcgtagaatcggtttgattgtcgttgtagtaatctacactatcggtatcattattcaaatagcctcgatcaacaagtggtaccaatatttcattggtagaattatctctggtttaggtgtcggtggtatcacagttttatctcccatgctaatatctgaggtcgcccccagtgaaatgagaggcaccttggtttcatgttaccaagtcatgattactttaggtattttcttaggttactgtaccaattttggtaccaagaattactcaaactctgtccaatggagagttccattaggtttgtgtttcgcctgggccttatttatgattggtggtatgatgtttgttcctgaatctccacgttatttggttgaagctggcagaatcgacgaagccagggcttctttagctaaagttaacaaatgcccacctgaccatccatacattcaatatgagttggaaactatcgaagccagtgtcgaagaaatgagagccgctggtactgcatcttggggcgaattattcactggtaaaccagccatgtttcaacgtactatgatgggtatcatgattcaatctctacaacaattaactggtgataactatttcttctactacggtaccattgttttccaggctgtcggtttaagtgactcttttgaaacttctattgtctttggtgtcgtcaacttcttctccacttgttgttctctgtacaccgttgaccgttttggccgtcgtaactgtttgatgtggggtgctgtcggtatggtctgctgttatgttgtctatgcctctgttggtgttaccagattatggccaaacggtcaagatcaaccatcttcaaagggtgctggtaactgtatgattgttttcgcatgtttctacattttctgtttcgctactacctgggccccaattgcttacgttgttatttcagaatgtttcccattaagagtcaaatccaagtgtatgtctattgccagtgctgctaactggatctggggtttcttgattagtttcttcaccccatttattactggtgccatcaacttctactacggttacgttttcatgggctgtatggttttcgcttacttttacgtctttttcttcgttccagaaactaaaggtttatcattagaagaagttaatgatatgtacgccgaaggtgttctaccatggaaatcagcttcctgggttccagtatccaagagaggcgctgactacaacgctgatgacctaatgcatgatgaccaaccattttacaagagtttgtttagcaggaaataatactagagcccgccgccaccatggagtactagatgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgcgagatacccagatcatatgaaacaacatgactttttcaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M11206_sequence/1
type: Sequence
persistent identity: BBa M11206 sequence
display id: BBa_M11206_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagactactagagcccgccgccaccatggagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165002_sequence/1
type: Sequence
persistent identity: BBa K165002 sequence
display id: BBa_K165002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M11202_sequence/1
type: Sequence
persistent identity: BBa M11202 sequence
display id: BBa_M11202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaactcaactcccgatctaatatctcctcagaaatccaattcatccaactcatatgaattggaatctggtcgttcaaaggccatgaatactccagaaggtaaaaatgaaagttttcacgacaacttaagtgaaagtcaagtgcaacccgccgttgcccctccaaacaccggaaaaggtgtctacgtaacggtttctatctgttgtgttatggttgctttcggtggtttcatatttggatgggatactggtaccatttctggttttgttgctcaaactgattttctaagaagatttggtatgaagcaccacgacggtagtcattacttgtccaaggtgagaactggtttaattgtctctatttttaacattggttgtgccattggtggtatcgtcttagccaagctaggtgatatgtatggtcgtagaatcggtttgattgtcgttgtagtaatctacactatcggtatcattattcaaatagcctcgatcaacaagtggtaccaatatttcattggtagaattatctctggtttaggtgtcggtggtatcacagttttatctcccatgctaatatctgaggtcgcccccagtgaaatgagaggcaccttggtttcatgttaccaagtcatgattactttaggtattttcttaggttactgtaccaattttggtaccaagaattactcaaactctgtccaatggagagttccattaggtttgtgtttcgcctgggccttatttatgattggtggtatgatgtttgttcctgaatctccacgttatttggttgaagctggcagaatcgacgaagccagggcttctttagctaaagttaacaaatgcccacctgaccatccatacattcaatatgagttggaaactatcgaagccagtgtcgaagaaatgagagccgctggtactgcatcttggggcgaattattcactggtaaaccagccatgtttcaacgtactatgatgggtatcatgattcaatctctacaacaattaactggtgataactatttcttctactacggtaccattgttttccaggctgtcggtttaagtgactcttttgaaacttctattgtctttggtgtcgtcaacttcttctccacttgttgttctctgtacaccgttgaccgttttggccgtcgtaactgtttgatgtggggtgctgtcggtatggtctgctgttatgttgtctatgcctctgttggtgttaccagattatggccaaacggtcaagatcaaccatcttcaaagggtgctggtaactgtatgattgttttcgcatgtttctacattttctgtttcgctactacctgggccccaattgcttacgttgttatttcagaatgtttcccattaagagtcaaatccaagtgtatgtctattgccagtgctgctaactggatctggggtttcttgattagtttcttcaccccatttattactggtgccatcaacttctactacggttacgttttcatgggctgtatggttttcgcttacttttacgtctttttcttcgttccagaaactaaaggtttatcattagaagaagttaatgatatgtacgccgaaggtgttctaccatggaaatcagcttcctgggttccagtatccaagagaggcgctgactacaacgctgatgacctaatgcatgatgaccaaccattttacaagagtttgtttagcaggaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K239006_sequence/1
type: Sequence
persistent identity: BBa K239006 sequence
display id: BBa_K239006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K194001_sequence/1
type: Sequence
persistent identity: BBa K194001 sequence
display id: BBa_K194001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgcgagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaatttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63002_sequence/1
type: Sequence
persistent identity: BBa J63002 sequence
display id: BBa_J63002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z