LOCUS BBa_M11400 7075 bp DNA linear 21-Dec-2024 DEFINITION Integration Plasmid for Synechocystis sp. PCC 6803 ACCESSION BBa_M11400 VERSION BBa_M11400.1 FEATURES Location/Qualifiers CDS 1..816 /label=Kanamycin Resistance (aphII) misc_feature 1..3 /label=Start misc_feature 814..816 /label=Stop misc_feature 1554..2922 /label=RS2 (recombination region for Synechocystis sp PCC 6803 - region of hypothetical gene slr0168) misc_feature 5272..6951 /label=RS1 (recombination region for Synechocystis PCC 6803 - region of hypothetical gene slr0338) CDS 3532..4392 /label=Ampicillin Resistance (bla) misc_feature 1292..1297 /label=PstI Site misc_feature 1267..1278 /label=BBa_B0042 misc_feature 3131..3140 /label=OriV dnaA box misc_feature 1336..1341 /label=EcoRI Site misc_feature 1321..1326 /label=XbaI Site misc_feature 1306..1311 /label=SpeI Site misc_feature 1355..1366 /label=BBa_B0042 misc_feature 1465..1536 /label=BBa_B0053 misc_feature 1198..1266 /label=BBa_B0054 misc_feature 1367..1444 /label=BBa_B0055 misc_feature 3532..3534 /label=Start misc_feature 1137..1177 /label=BBa_B0062 misc_feature 4390..4392 /label=Stop misc_feature 1321..1341 /label=BBa_G00000 misc_feature 1292..1311 /label=BBa_G00001 primer_bind 1446..1464 /label=BBa_G00100 (VF2) primer_bind 1178..1197 /label=BBa_G00102 (VR) ORIGIN 1 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat 61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 121 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 541 gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 781 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 841 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt 901 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa 961 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct 1021 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt 1081 cttcacgagg cagacctcag cgcccccccc ccccagcagc ggccgctact agctagagaa 1141 atcatcctta gcgaaagcta aggatttttt ttatctgatt accgcctttg agtgagcccc 1201 aatgataacc ccaagggaag ttttagtcaa aagcctccgg tcggaggctt ttgactttct 1261 gctaatctaa ctaactaacc cttagtgact cctgcagcgg ccgctactag tagcggccgc 1321 tctagaagcg gccgcgaatt cgagtcacta agggctaact aactaattac gtagcaatca 1381 actcactggc tcaccttcac gggtgggcct ttcttcggca cgggcaaatt gctgaatatt 1441 ccttttctta gacgtcaggt ggcaaacgcg aagtaatctt ttcggtttta aagaaaaagg 1501 gcagggtggt gacaccttgc ccgttttttt gccggagcta gctagaagcg gccgccaatt 1561 cccggtatgg atggcaccga tgcggaatcc caacagattg cctttgacaa caatgtggcc 1621 tggaataacc tgggggattt gtccaccacc acccaacggg cctacacttc ggctattagc 1681 acagacacag tgcagagtgt ttatggcgtt aatctggaaa aaaacgataa cattcccatt 1741 gtttttgcgt ggcccatttt tcccaccacc cttaatccca cagattttca ggtaatgctt 1801 aacacggggg aaattgtcac cccggtgatc gcctctttga ttcccaacag tgaatacaac 1861 gaacggcaaa cggtagtaat tacgggcaat tttggtaatc gtttaacccc aggcacggag 1921 ggagcgattt atcccgtttc cgtaggcaca gtgttggaca gtactccttt ggaaatggtg 1981 ggacccaacg gcccggtcag tgcggtgggt attaccattg atagtctcaa cccctacgtg 2041 gccggcaatg gtcccaaaat tgtcgccgct aagttagacc gcttcagtga cctgggggaa 2101 ggggctcccc tctggttagc caccaatcaa aataacagtg gcggggattt atatggagac 2161 caagcccaat ttcgtttgcg aatttacacc agcgccggtt tttcccccga tggcattgcc 2221 agtttactac ccacagaatt tgaacggtat tttcaactcc aagcggaaga tattacggga 2281 cggacagtta tcctaaccca aactggtgtt gattatgaaa ttcccggctt tggtctggtg 2341 caggtgttgg ggctggcgga tttggccggg gttcaggaca gctatgacct gacttacatc 2401 gaagatcatg acaactatta cgacattatc ctcaaagggg acgaagccgc agttcgccaa 2461 attaagaggg ttgctttgcc ctccgaaggg gattattcgg cggtttataa tcccggtggc 2521 cccggcaatg atccagagaa tggtccccca gggcccttta ctgtgtccag tagtccccag 2581 gtaattaagg taacggatac catcggccag cccaccaaag tctcctatgt ggaagtggat 2641 ggccccgtat tgcgtaatcc cttcagtggt actcccattg ggcaagaggt gggtttagcg 2701 gttaaagatc tggccacagg tcatgaaatt tatcagtaca ctgacccaga tgggaaggta 2761 ttttatgctt cctttgctgc cgctgatgac caagccacgg atttaaccac ggcgatcgcc 2821 aatcccacgg ccatcgattt aattaacgcc aggggattta cggcgggtag ttccgtcacc 2881 gtatcgggtt cctacagtcg ggaagccttt tttgatggat ccccgggaat taattcactg 2941 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 3001 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 3061 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 3121 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 3181 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 3241 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3301 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 3361 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 3421 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3481 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3541 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 3601 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 3661 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 3721 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 3781 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3841 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3901 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3961 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 4021 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 4081 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4141 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4201 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4261 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4321 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4381 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4441 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4501 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4561 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 4621 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 4681 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 4741 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 4801 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4861 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4921 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa 4981 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5041 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5101 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5161 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5221 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagttcga cggatcctgg 5281 gcttcggcta tggtggcgta aagggctgga gttagaccaa caaaggccgt ggtcagtaaa 5341 gaaagggaag cggtcagagg acgaatattc ggcatagata aattccttga tgtaagtttg 5401 gtaaagtttt tcgatcaaat caccaaaatc agttccagca caattattgg ggtcagtttt 5461 aaccccggtc cagaaaatcg gcgataatct cccctgggtc ggtttttgac tggggaaaga 5521 agagaaacac ggcctgtgct agtatctaag gcatgaattt tcgtgaagtt aattataaca 5581 gtcagtattt tgcggctggc taggtgttta ggagtggcaa cagcatcatg acgagccatc 5641 agctcaacgg gcaacggagt taccttcagc cgatccgcat cggggtgatt ggggtgggca 5701 atatgggaca gcaccatacc agagtcctga gcctgatgaa ggatgtggag tttgtgggta 5761 ttgccgatgt caatgtggaa cggggcctag acaccgccag taagtatcgg gtgcattttt 5821 ttgaagatta tcaggagatg ttgccccatg tggatgcggt ttgtgtggcg gttcccacta 5881 ggctccatca cgatgtgggc atgaattgtt tgcaaaataa tgtccatact ctgattgaaa 5941 aacccattgc cgctagcatt gctgaagcgg aatccctggt taatgccgcc gccgatgcca 6001 attgcattct ccaagtgggg cacattgaac gcttcaaccc ggcattttta gagctaacca 6061 aaattctcaa aacggaagag ttattggcga tcgaagccca tcgcatgagt ccctattccc 6121 agcgggccaa tgatgtctcc gtggtattgg atttgatgat ccatgacatt gacctgttgc 6181 tggaattggt gggttcggaa gtggttaaac tgtccgccag tggcagtcgg gcttctgggt 6241 caggatattt ggattatgtc accgctacgt taggcttctc ctccggcatt gtggccaccc 6301 tcaccgccag taaggtcacc catcgtaaaa ttcgttccat cgccgcccac tgcaaaaatt 6361 ccctcaccga agcggatttt ctcaataacg aaattttgat ccatcgccaa accaccgctg 6421 attggagcgc ggactatggc caggtattgt atcgccagga tggtctaatc gaaaaggttt 6481 acaccagtaa tattgaacct ctccacgctg aattagaaca ttttattcat tgtgttaggg 6541 gaggtgatca accctcagtg gggggagaac aggccctcaa ggccctgaag ttagccagtt 6601 taattgaaga aatggccctg gacagtcagg aatggcatgg gggggaagtt gtgacagaat 6661 atcaagatgc caccctggcc ctcagtgcga gtgtttaaat caacttaatt aatgcaatta 6721 ttgcgagttc aaactcgata actttgtgaa atattactgt tgaattaatc tatgactatt 6781 caatacaccc ccctagccga tcgcctgttg gcctacctcg ccgccgatcg cctaaatctc 6841 agcgccaaga gtagttccct caacaccagt attctgctca gcagtgacct attcaatcag 6901 gaagggggaa ttgtaacagc caactatggc tttgatggtt atatgggaat tgcggggggg 6961 gggggaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat 7021 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgtt //