BBa_M11400 1 BBa_M11400 Integration Plasmid for Synechocystis sp. PCC 6803 2010-04-23T11:00:00Z 2015-05-08T01:13:53Z Genomic Sequence, Genbank This plasmid is designed to integrated into the Synechocystis sp. PCC 6803 genome. It contains an ampicillin resistance gene and an E. coli origin of replication, so BioBrick parts can be assembled in E. coli, then transformed into Synechocystis for genomic integration. In Synechocystis, transformed cells can be selected with kanamycin. M11400 is based on plasmid pIGA from Kunert et al. (2000), GenBank accession number AJ251839.1. The recombination sites correspond to the regions containing hypothetical proteins slr0338 and slr0168 in Synechocystis sp. PCC 6803. false false _572_ 0 6788 9 Not in stock false Site-directed mutagenesis of EcoRI site between RS1 and kanamycin resistance gene. Removal of GFP expression cassette and T7 terminator. Addition of translation and transcription stop sequences flanking BioBrick prefix and suffix sites (from BioBrick Basic Vector BBa_I51020). false Cody Tramp annotation2068502 1 SpeI Site range2068502 1 1306 1311 annotation2068507 1 BBa_B0053 range2068507 1 1465 1536 annotation2068501 1 XbaI Site range2068501 1 1321 1326 annotation2068531 1 BBa_G00001 range2068531 1 1292 1311 annotation2068497 1 PstI Site range2068497 1 1292 1297 annotation2068523 1 BBa_B0055 range2068523 1 1367 1444 annotation2068492 1 Start range2068492 1 1 3 annotation2068503 1 BBa_B0042 range2068503 1 1355 1366 annotation2068530 1 BBa_G00000 range2068530 1 1321 1341 annotation2068496 1 Ampicillin Resistance (bla) range2068496 1 3532 4392 annotation2068532 1 BBa_G00100 (VF2) range2068532 1 1446 1464 annotation2068533 1 BBa_G00102 (VR) range2068533 1 1178 1197 annotation2068498 1 BBa_B0042 range2068498 1 1267 1278 annotation2068526 1 Start range2068526 1 3532 3534 annotation2068495 1 RS1 (recombination region for Synechocystis PCC 6803 - region of hypothetical gene slr0338) range2068495 1 5272 6951 annotation2068491 1 Kanamycin Resistance (aphII) range2068491 1 1 816 annotation2068508 1 BBa_B0054 range2068508 1 1198 1266 annotation2068529 1 Stop range2068529 1 4390 4392 annotation2068493 1 Stop range2068493 1 814 816 annotation2068528 1 BBa_B0062 range2068528 1 1137 1177 annotation2068494 1 RS2 (recombination region for Synechocystis sp PCC 6803 - region of hypothetical gene slr0168) range2068494 1 1554 2922 annotation2068499 1 OriV dnaA box range2068499 1 3131 3140 annotation2068500 1 EcoRI Site range2068500 1 1336 1341 BBa_M11400_sequence 1 atgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaaccgtggctccctcactttctggctggatgatggggcgattcaggcctggtatgagtcagcaacaccttcttcacgaggcagacctcagcgccccccccccccagcagcggccgctactagctagagaaatcatccttagcgaaagctaaggattttttttatctgattaccgcctttgagtgagccccaatgataaccccaagggaagttttagtcaaaagcctccggtcggaggcttttgactttctgctaatctaactaactaacccttagtgactcctgcagcggccgctactagtagcggccgctctagaagcggccgcgaattcgagtcactaagggctaactaactaattacgtagcaatcaactcactggctcaccttcacgggtgggcctttcttcggcacgggcaaattgctgaatattccttttcttagacgtcaggtggcaaacgcgaagtaatcttttcggttttaaagaaaaagggcagggtggtgacaccttgcccgtttttttgccggagctagctagaagcggccgccaattcccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtgacctgggggaaggggctcccctctggttagccaccaatcaaaataacagtggcggggatttatatggagaccaagcccaatttcgtttgcgaatttacaccagcgccggtttttcccccgatggcattgccagtttactacccacagaatttgaacggtattttcaactccaagcggaagatattacgggacggacagttatcctaacccaaactggtgttgattatgaaattcccggctttggtctggtgcaggtgttggggctggcggatttggccggggttcaggacagctatgacctgacttacatcgaagatcatgacaactattacgacattatcctcaaaggggacgaagccgcagttcgccaaattaagagggttgctttgccctccgaaggggattattcggcggtttataatcccggtggccccggcaatgatccagagaatggtcccccagggccctttactgtgtccagtagtccccaggtaattaaggtaacggataccatcggccagcccaccaaagtctcctatgtggaagtggatggccccgtattgcgtaatcccttcagtggtactcccattgggcaagaggtgggtttagcggttaaagatctggccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccccgggaattaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagttcgacggatcctgggcttcggctatggtggcgtaaagggctggagttagaccaacaaaggccgtggtcagtaaagaaagggaagcggtcagaggacgaatattcggcatagataaattccttgatgtaagtttggtaaagtttttcgatcaaatcaccaaaatcagttccagcacaattattggggtcagttttaaccccggtccagaaaatcggcgataatctcccctgggtcggtttttgactggggaaagaagagaaacacggcctgtgctagtatctaaggcatgaattttcgtgaagttaattataacagtcagtattttgcggctggctaggtgtttaggagtggcaacagcatcatgacgagccatcagctcaacgggcaacggagttaccttcagccgatccgcatcggggtgattggggtgggcaatatgggacagcaccataccagagtcctgagcctgatgaaggatgtggagtttgtgggtattgccgatgtcaatgtggaacggggcctagacaccgccagtaagtatcgggtgcatttttttgaagattatcaggagatgttgccccatgtggatgcggtttgtgtggcggttcccactaggctccatcacgatgtgggcatgaattgtttgcaaaataatgtccatactctgattgaaaaacccattgccgctagcattgctgaagcggaatccctggttaatgccgccgccgatgccaattgcattctccaagtggggcacattgaacgcttcaacccggcatttttagagctaaccaaaattctcaaaacggaagagttattggcgatcgaagcccatcgcatgagtccctattcccagcgggccaatgatgtctccgtggtattggatttgatgatccatgacattgacctgttgctggaattggtgggttcggaagtggttaaactgtccgccagtggcagtcgggcttctgggtcaggatatttggattatgtcaccgctacgttaggcttctcctccggcattgtggccaccctcaccgccagtaaggtcacccatcgtaaaattcgttccatcgccgcccactgcaaaaattccctcaccgaagcggattttctcaataacgaaattttgatccatcgccaaaccaccgctgattggagcgcggactatggccaggtattgtatcgccaggatggtctaatcgaaaaggtttacaccagtaatattgaacctctccacgctgaattagaacattttattcattgtgttaggggaggtgatcaaccctcagtggggggagaacaggccctcaaggccctgaagttagccagtttaattgaagaaatggccctggacagtcaggaatggcatgggggggaagttgtgacagaatatcaagatgccaccctggccctcagtgcgagtgtttaaatcaacttaattaatgcaattattgcgagttcaaactcgataactttgtgaaatattactgttgaattaatctatgactattcaatacacccccctagccgatcgcctgttggcctacctcgccgccgatcgcctaaatctcagcgccaagagtagttccctcaacaccagtattctgctcagcagtgacctattcaatcaggaagggggaattgtaacagccaactatggctttgatggttatatgggaattgcggggggggggggaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z