LOCUS BBa_M11421 11889 bp DNA linear 18-Nov-2024 DEFINITION Streptomyces Shuttle Vetor Thiostrepton Resistance ACCESSION BBa_M11421 VERSION BBa_M11421.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 11867..11889 /label=Prefix CDS 382..1188 /label=Thiostrepton Resistance misc_feature 382..384 /label=Start Codon misc_feature 1189..1191 /label=Stop Codon misc_feature 1554..8594 /label=oriSCP2 ORIGIN 1 tactagtagc ggccgctgca gaagcttcgg ccacgacacc cccatcggca tcgcgtggcg 61 ggcccgattc gcgtccgccc cggggaggag acgggcgtcc gcgtcgaggg cgaggcgggc 121 ttccacccgt aggcgatccg gcgagcacga cacccgcaga ttggctcgac gcagcccaga 181 aatgtatgat caaggcgaat acttcatatg cggggatcga ccgcgcgggt cccggacggg 241 gaagagcggg gagctttgcc agagagcgac gacttcccct tgcgttggtg attgccggtc 301 agggcagcca tccgccatcg tcgcgtaggg tgtcacaccc caggaatcgc gtcactgaac 361 acagcagccg gtaggacgac catgactgag ttggacacca tcgcaaatcc gtccgatccc 421 gcggtgcagc ggatcatcga tgtcaccaag ccgtcgcgat ccaacataaa gacaacgttg 481 atcgaggacg tcgagcccct catgcacagc atcgcggccg gggtggagtt catcgaggtc 541 tacggcagcg acagcagtcc ttttccatct gagttgctgg atctgtgcgg gcggcagaac 601 ataccggtcc gcctcatcga ctcctcgatc gtcaaccagt tgttcaaggg ggagcggaag 661 gccaagacat tcggcatcgc ccgcgtccct cgcccggcca ggttcggcga tatcgcgagc 721 cggcgtgggg acgtcgtcgt tctcgacggg gtgaagatcg tcgggaacat cggcgcgata 781 gtacgcacgt cgctcgcgct cggagcgtcg gggatcatcc tggtcgacag tgacatcacc 841 agcatcgcgg accggcgtct ccaaagggcc agccgaggtt acgtcttctc ccttcccgtc 901 gttctctccg gtcgcgagga ggccatcgcc ttcattcggg acagcggtat gcagctgatg 961 acgctcaagg cggatggcga catttccgtg aaggaactcg gggacaatcc ggatcggctg 1021 gccttgctgt tcggcagcga aaagggtggg ccttccgacc tgttcgagga ggcgtcttcc 1081 gcctcggttt ccatccccat gatgagccag accgagtctc tcaacgtttc cgtttccctc 1141 ggaatcgcgc tgcacgagag gatcgacagg aatctcgcgg ccaaccgata agcgcctctg 1201 ttcctcggac gctcggttcc tcgacctcga ttcgtcagtg atgatcacct cacacggcag 1261 cgatcaccac tgacatatcg aggtcaacgg tcgtggtccg ggcgggcact cctcgaaggc 1321 gcggccgacg cccttgaacg actcgatggc gctgacgcct tccgcgatcg cctcgactgt 1381 gaaaacgctc gtggacgtcg gcgggtagcc gatgagttcc atcgccttgc gcatcccctt 1441 gagcatggag aacgcgtcca ccgggtagtc acgaaggtac ttgcgaaggc ttcgcgccgt 1501 ccgagaccgt gagccggcgc aagccggtgt ggcgccggtc ccggatttgc aggcatgcct 1561 cgagtccgca gtcctggcag gcagagcagg tccggcagtg gccacagccg cggttgtcgg 1621 gcggccagca gcggcagccg gtgggctgcc aggggtcggc ggagccgacc agcccgagct 1681 ggccgggcac gttctcctcg ggcgctcggc gcggcgcgcg tggcttgacg cggctgcggg 1741 ccatcagcgg gcctccgggg tgtagccctc ggcgaggagc tgcgcggcgg ccgaccggta 1801 ctccgaggcc gtcgtctgcg agacggagca ggcggtcatg accgactcca gggggaggtt 1861 ctccgggtgt ccggcgccct cggcgaggat catccgggcc accttgcgga ccttgcggtc 1921 acgcggggag agggtcagtt cgtcctcggc ttcgaccgcc cggcgctccg cttcggccgc 1981 gcggtggcgg gcttcggcgg cggcctgctc ggcttcggct gcgtcccgct gtgcctggga 2041 ggcgcgggcc cgggcctcct cggtgctggc ctcggcggcc gccgtgcggg cctcgtcttc 2101 ggccgcagtg agggccgctt cggcttcgcg ggccctggct tcggctgcgg cctcgtcggc 2161 ttcggccgcg cggcggcggg cttcggcggc gcggtgctcg gcggccgcgc ggtcggcttc 2221 ggcccgggcg gcggcctccg actggacggc ctccgtctcg gcggtcgcgg cccgctcggc 2281 ggcggcgacc tcggcggccg cctggacctc cgcgcggctg gtcgacgcat tcacctcggt 2341 acgggcggct tggacggctc cagcggcctt aaggcgggcg atctcggcgg cggcggcccg 2401 ctcctgcttg cgggcctcct cggcgagcct ggcgtcctcc tcggcctccc ggagggcctg 2461 cgcgcgctcc tcggcctgcc gggggagcgc cagggcctcc gcgatggtca ggccgtaccg 2521 ctccatggtg aggggcagcc ggacgtccga gggggccttg cggacgctgc cgtattcgcg 2581 ttccagcatg acgcggtaga cgcgcagctc cttgtccagc tccacggcct ccgcgtaggt 2641 ggcgaccgcc cacagcttca tgcggcgcca cagggcgaag gtcggggcgg gggcgaggaa 2701 ccagcggtag agcgggacgc ccgggccgtt gtgggcgtcg gggcggacca cgcggtcaac 2761 ggcggtcttg ccgacctcca cgatggcgat gaacagcacg ggcacgatcg cgtgggcgaa 2821 cgtggccacg tagtccaggg cggtccaggc gccggccgac ggggcggcgg aggccgcgtt 2881 gaggacgatc gttgcgccgg tcagcagcca cacgggaaag cggacccagc cgatggggcg 2941 gcgcagccac gccatgagca ggtcgagggc gatcaggacc aggatgccga cgtcgacgcc 3001 gaccgtgaac agccgggcct tgcccagcgt ggcgaacccg agccggtcgt gcgcgaacgt 3061 ggcgacgtgc tcgaaggaga ggtagaggcc gatcccggcg agcgccaggg cggcgaggac 3121 gatcgctccg gcggtggccg actgcgcccg ggtgagcccg ccggggagac tccagcggcg 3181 gcccgtgccc ggagccgtcg cggtcgtctc cggagccggg gcggccgggc tcgaagcggc 3241 ggccgtcgtg gccgaagccg tgggcggcgc gtccgaagcg gggcgcggcg aggacggagt 3301 ggggccgggg cgctgcttgc tgaggggcag ggtggtgagg gtcatcaggt cctccgggtg 3361 atggtcgggc gaagtgtccg aagcggcggc cgccgccccc gaagccgggg tgtggcggcc 3421 gccgtggcgc ccgaaggtcg gtcaggtccg gccgtctcgc ccggcggtga ggaccagggc 3481 gagggtggcg agcgcggcgc cgtacgccga caagcaggtg agcaccgtgg gcacggacgg 3541 ctggacgatt gagacggcca gccaggccgt ggcgtgcacg gtgaggacca ggacggcggc 3601 ggcctcgcgg acggtcagcc gacgcggagg ccggcggccg gtcaccatcg gcgctgccgg 3661 atgacccggc ggcgggcggc ggggtcccgg ggcagcggcg gcatcggggt cggcgtcggc 3721 acgacgacga tgcggcggcg ctgcgggggg acggtgcgct gcggctgctc ggggcggtcg 3781 ggcacggcgg tcaccgcttg ccctggcgga cgcggcgcag ggcctcgtcc atgtccgggt 3841 cggagtcggt ggggcaggtg gccaggttgt tggtacggtc cgtcatgcgt cgttcctcct 3901 ggtgattcgg ttggtgcgcg cgggcacggc ccccggttca ggcgctgcta tcgcctcggg 3961 ggccgtcgtc gtgtctggtc agccgcgggg gccgggaagg ccggcgcccc ggtggcgggg 4021 gcactggcag ggcggccagg ggccgggccc aaggcccagc ggccccgcgt tctcccatgc 4081 cgtgtcgtgg tcgacggcct cggtcacggc gctgccgtac gagcggccgc cgtccgtgct 4141 cacccggagg tcatccggga gagtgtgccg ccggggtccg ggtgctcgcg gagctggtcc 4201 cggatggcgt cggcggtgcg gccgtagagc ggctggtcga tcgggtgcgc gtgaacgtca 4261 tcggtcatgc ggtcggtccc tctcgtctcg tgcgaaggcc cccggcgggt gccgggggcc 4321 ggtggggggt gaggtctggt caggcggcga gctgcgccgt ggcggcctcg tcggcggccg 4381 ggaagggcac gacgtcagcg gcggcccggg tgacgcgcag cgagggcgcg caggtccgca 4441 tggggacgtc cccgagcccg agccgcttgt aggtggcgcg gatggcctcc aggtccgggg 4501 tctgccgcga ggacgggacg cgctccacgg tccagacgcc gtaacgcccg gcgggaaggc 4561 ggtccaggag cttgcgggcc ttgcgcttgc cccggtctcc ggagcgggcc cggtcggtgg 4621 cggcggcgta gtcggctgcc gcgtcctcga tctcctcggc gcgggcctcg atctcctccg 4681 gagccaggag gccggtcggg tcgggcgccg tctcgtcggc gcgggagcgg accagggagg 4741 ccggggccac ggtggagcgg acggcgtcgg cgacggtgcg gaaggcgcga cgcgcgcggg 4801 tggtgacgct acggatcatg gtggtgcctc ctggtgactg gtgctggtcg tgcgagggcc 4861 accggggcgc tgctatcgcc cctggcggcc ggggtgccga gtaggcgcgg ctccggcacg 4921 acgcgcgtga cgcgcgaggt gctggaccgc gacgactcgg agccgtcggt gccggtcttc 4981 accgccatgt ttccgacctc ccacacgctc gaaggctggc ggtccacggg ttaccggcgt 5041 ggtccctgat ctgcggttat caacgtgctg tcgcttcctt cgagaccacc gtcagtgctg 5101 tccctccggg cgttgagtga tcacctcgct tcggtgcgtg gtggcccctc tgggcttaag 5161 tagagcacta cacttaagga atcgcaagca ctacactcaa gagcgtcagc acgatgagca 5221 ggagggaccg atggcgacta ggcggaaggg ccgccctggc ggctatgagg aaatcgccgc 5281 gcactttcgg cggctcatgg actcgggcga gttgtcccct ggcgacccgc tgccctccat 5341 gcgcgacgtg tgcgaccagt tcggttcggc gatcacgacg gtgaaccggg cgttccggct 5401 cctccaggag gagggccgga cggtctccaa gccgggcgtg ggcacgatcg tccgggacat 5461 gtcccgggtt cgggtgccgt tcagtacgta cggcgacgtc ctggcgccgg gcggcgataa 5521 gggcccgtgg gagcgtgcga cggccgcgca gggccttgac ggccggatgc tcgtggaggc 5581 gcccgaggag gtcggggccc cggcggacgt cgccgcgcgc ctcggcatcg agccgggcgc 5641 cctggtcgtc caccggcggc gccgcgccac gatcggcgag gacgtcgtcc agctccaaga 5701 cgcctggtac ccgctggaga tcgcccgggc cgccggcctg gaccggccgg ggaaggtcgt 5761 gggtggtgtc ctcggtgcca tgacgggcgc cggcctttcg ccgacgtcca ccgaccacga 5821 cgtcgaggtg tgggtgccgt ccgcgcagca agccgcggaa ctctccctcg gctcccgcgt 5881 gtcggtcctg gtcgtcgagc gcgtcaccta cgacgcgacg gtccgtgtcc tggaactgac 5941 ccgtcacacg ggcgcggctg acaggctgac gctgacctac aagggcctgc cactccgggc 6001 gaccggagcc gaggggagca cgtcatgacg aacgagctgg acaccgccag cgcggccgcg 6061 caggagacgc tggagaggtc ggcggcggaa ctggtccgcc tccaggtggt gcgggagcta 6121 cgcgccacgc acggcctggt ctaccgggcg ctggacggca tggagccgcc ctatctggtg 6181 gcgtgggagg gccggttcgt tggcgaggtg caccggcacg gtccggccta tctgcactcc 6241 tggtacgtgc tgccgtacgg cgacgacgag gagccgacgg cggaggcccc cgacttcaac 6301 ggccgtccgt tcggcacggc ccgggccgcc gccgcgtcgc tcaagccagg ccaggcgtag 6361 gccggccccg cccggacacg agaccgcccc ccttccgcag ccaccggaac gggggcggtc 6421 ctatgccgca ccagagccaa cacacccttg gaggatcacc ccgtgaccca atcccggaag 6481 ccctcggaag tcgtctatct cttaggagcc gagggcctag acctggtgaa gatcggcacc 6541 acgacagacg tggagaggag agtccgcacg atgcagaccg gtctgccgct gacgctgtcg 6601 gtgctgtgga cgtgcgaggg cggccgcgcc cttgaggggg cgctccacca ggagttccgt 6661 gagcacaacc ggcggggcga gtggttcgac ctgaccagcc ttggtgaccc ggtggccgtc 6721 gtgagtgaga cggtgcggag gcttgcgcca ggtcttgggc tcccgatccc gccgccgcgc 6781 gtctcgccgg acctggctct aacgcctgtg gcccccacga ttccggggtg cgtccggctg 6841 gagcgcaccc tgaccgctcg gatgctcgcc tggttcgagt gcgaagactt cggccttccg 6901 ccctccgcga gcaagcctgt gatgctgtac cggaccgtcc agtacgacgc gaagggcgac 6961 gtcctggccg tgacgcagac gctcgttggc gatggacacg agcagcctga aggcttcgag 7021 cttcctcccg tacagggcgg caactgaccg tctggcacat agatcagcgc ccccgctccg 7081 tgggagatcg gagcgggggc gctgtgttgt gcggtcaaga gacggggcgg atcgtccggc 7141 ggcggcgagc gtcggtgttg ctgaccttgc ggggcgcgcg ggcacggtcg tccggggtga 7201 ccggggggtc cgtgagggcg gcggtgatca tctcgcgcag ctcggggtgc agcgtgaagt 7261 agtaccgggc gttgatccgg tagaggccgg cggaggtccg cacgaggagg ttcagggcct 7321 ccaggcgggc gagagcctcg tgcaccgtgg agacggagtt gccgcagtcc tcggcgacgg 7381 tttcgaccgg tacggggatg acgtcaaaca ggccgccgtg gtcctccacc cacgcgagcg 7441 tgcagtgctg agcggcggac aggttcggcg agtacagcga cggggcggtc aggtcgcgct 7501 ccgggttgaa gccttctacg ggcatggtcg tcatcgcgtc tctcctgcgg cgcggcggcg 7561 ggtagccggg atggtcttgg gcttgctggc ggacctggtc atggccttgt cgtccggggc 7621 cacacgcggc ggcgccatgc ggcggcaggc gttgacctgc gtgctcccgg ctccctccca 7681 gtatcggagg gggttcagct ggtaagagcc ggatcgcggg cccggcttga ccacaagccc 7741 gagcttgacc agccggttaa gggccttccg gcaggtgtcc ttgctggcgc cgacgtcctc 7801 gcccagctcc tcgggcgtga ccgggacgta tccggccgcg cctccgtgtt cgcggagcca 7861 ttcgagcgtc gcccactccg ctgcggccag cccgagggag tacaggggga cgtcgctgcc 7921 tacggcctgc aaggtgtacc ccttcccgtc gaactggtgc ggagtccgcg gcctggcgga 7981 caccgcctct ccggtgtcca tgttgaccag ggccatgttc ggcccgcgct tcctcgtcgg 8041 cactcgcgcc tctcccatcc tcgcgctaag gggcggtttt tgacccctta gctccgatga 8101 accgccgctt acgagaggaa actaacaggt caccactccg aaaaacagcc gcttagtccg 8161 cgtgtcacac gcataggtga cgcgacttcc tggcgaccgg gcgcgacgag ggacgcacgg 8221 tgggtagcta agcggcgatt tttgacccag taaccggcgg tttttagccg cttagccgcg 8281 cgttttacca ggtcaagagc atgcgcccct tcttcactag aaggcgcgcg ccgtcactca 8341 cgcaccacgg cgcacaccgt ggtgaccgcc ctggactcct acccgcccgg cataccgggc 8401 cgctcttagc ttctacgggg aaggccggcg gtcctgcgca cctggtcctc gtcggcgcct 8461 acggtcggcg agtcctcagc cctggcgggc ttgcgggtcc cgactgatgc cacctgatca 8521 cgcctggcga ccgcacgctg tcctcggccg gccacaagca ggaaggaagc gtcgtcctgc 8581 cggtgggcgg ctgaggtacc tccgtaccgc cgccgttgtc gcccccgccg caggggttgc 8641 ctttctgtgg ctgaggtcga ttcccccgcc attgatcacc cgcaccaggg agggcccagc 8701 cgacgaggtg gcgcgcgtgc gcacgtcgct gggtgggggt gcggtaggta ccgggcagca 8761 gctcggggac caggccccgg cgggcggggg tccggaggag cccgccgggg gtgtgcgcaa 8821 gggcgtagcc cgtagccccg gagcgcagcg gaggggtcgc tagtaggagg ccgctatggc 8881 gcgtaccacc agcccacagg gtccggcctc cggaggcgac gcactgccgg accggaccgc 8941 aaggtccggt gagcccacaa cggcggatca accgataaga cggccaccgg gcaggaagag 9001 ggtgtacgcg ccccttggtg gcccctctcg cgcgcgatca cctcacggcg cgtctcccag 9061 gtagtcagat ccgtgcgggg ttctctacgg gccgcacacg gccttcgtcc taagaggctc 9121 ttttccgccg cttagctccg aggaaccgcc ccttatctca atgaaagcgc aggtcaccac 9181 tccaggaaac ggccgggcga gcgaccagcc aggaggggag ccgcggatgc gccggtcctc 9241 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga 9301 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 9361 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc 9421 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg 9481 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg 9541 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc 9601 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg 9661 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca 9721 ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg 9781 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat 9841 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac 9901 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg 9961 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggccg 10021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 10081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 10141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 10201 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt 10261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 10321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 10381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 10441 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 10501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 10561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 10621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 10681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 10741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 10801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 10861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 10921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 10981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 11041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 11101 ccattgcagc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 11161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 11221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 11281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 11341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 11401 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 11461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 11521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 11581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 11641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 11701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 11761 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 11821 ataaaaatag gcgtatcacg aggccctttc gtcttcaatg catctatgaa ttcgcggccg 11881 cttctagag //