##gff-version 3
##sequence-region BBa_M11421 1 11889
BBa_M11421	.	stop_codon	1189	1191	.	+	0	ID=annotation2068724;Name=Stop Codon
BBa_M11421	.	CDS	382	1188	.	+	0	ID=annotation2068722;Name=Thiostrepton Resistance
BBa_M11421	.	sequence_feature	1554	8594	.	+	0	ID=annotation2068725;Name=oriSCP2
BBa_M11421	.	engineered_region	11867	11889	.	+	0	ID=annotation2068721;Name=Prefix
BBa_M11421	.	start_codon	382	384	.	+	0	ID=annotation2068723;Name=Start Codon
BBa_M11421	.	engineered_region	1	21	.	+	0	ID=annotation2068720;Name=Suffix
>BBa_M11421
tactagtagcggccgctgcagaagcttcggccacgacacccccatcggcatcgcgtggcgggcccgattcgcgtccgcc
ccggggaggagacgggcgtccgcgtcgagggcgaggcgggcttccacccgtaggcgatccggcgagcacgacacccgca
gattggctcgacgcagcccagaaatgtatgatcaaggcgaatacttcatatgcggggatcgaccgcgcgggtcccggac
ggggaagagcggggagctttgccagagagcgacgacttccccttgcgttggtgattgccggtcagggcagccatccgcc
atcgtcgcgtagggtgtcacaccccaggaatcgcgtcactgaacacagcagccggtaggacgaccatgactgagttgga
caccatcgcaaatccgtccgatcccgcggtgcagcggatcatcgatgtcaccaagccgtcgcgatccaacataaagaca
acgttgatcgaggacgtcgagcccctcatgcacagcatcgcggccggggtggagttcatcgaggtctacggcagcgaca
gcagtccttttccatctgagttgctggatctgtgcgggcggcagaacataccggtccgcctcatcgactcctcgatcgt
caaccagttgttcaagggggagcggaaggccaagacattcggcatcgcccgcgtccctcgcccggccaggttcggcgat
atcgcgagccggcgtggggacgtcgtcgttctcgacggggtgaagatcgtcgggaacatcggcgcgatagtacgcacgt
cgctcgcgctcggagcgtcggggatcatcctggtcgacagtgacatcaccagcatcgcggaccggcgtctccaaagggc
cagccgaggttacgtcttctcccttcccgtcgttctctccggtcgcgaggaggccatcgccttcattcgggacagcggt
atgcagctgatgacgctcaaggcggatggcgacatttccgtgaaggaactcggggacaatccggatcggctggccttgc
tgttcggcagcgaaaagggtgggccttccgacctgttcgaggaggcgtcttccgcctcggtttccatccccatgatgag
ccagaccgagtctctcaacgtttccgtttccctcggaatcgcgctgcacgagaggatcgacaggaatctcgcggccaac
cgataagcgcctctgttcctcggacgctcggttcctcgacctcgattcgtcagtgatgatcacctcacacggcagcgat
caccactgacatatcgaggtcaacggtcgtggtccgggcgggcactcctcgaaggcgcggccgacgcccttgaacgact
cgatggcgctgacgccttccgcgatcgcctcgactgtgaaaacgctcgtggacgtcggcgggtagccgatgagttccat
cgccttgcgcatccccttgagcatggagaacgcgtccaccgggtagtcacgaaggtacttgcgaaggcttcgcgccgtc
cgagaccgtgagccggcgcaagccggtgtggcgccggtcccggatttgcaggcatgcctcgagtccgcagtcctggcag
gcagagcaggtccggcagtggccacagccgcggttgtcgggcggccagcagcggcagccggtgggctgccaggggtcgg
cggagccgaccagcccgagctggccgggcacgttctcctcgggcgctcggcgcggcgcgcgtggcttgacgcggctgcg
ggccatcagcgggcctccggggtgtagccctcggcgaggagctgcgcggcggccgaccggtactccgaggccgtcgtct
gcgagacggagcaggcggtcatgaccgactccagggggaggttctccgggtgtccggcgccctcggcgaggatcatccg
ggccaccttgcggaccttgcggtcacgcggggagagggtcagttcgtcctcggcttcgaccgcccggcgctccgcttcg
gccgcgcggtggcgggcttcggcggcggcctgctcggcttcggctgcgtcccgctgtgcctgggaggcgcgggcccggg
cctcctcggtgctggcctcggcggccgccgtgcgggcctcgtcttcggccgcagtgagggccgcttcggcttcgcgggc
cctggcttcggctgcggcctcgtcggcttcggccgcgcggcggcgggcttcggcggcgcggtgctcggcggccgcgcgg
tcggcttcggcccgggcggcggcctccgactggacggcctccgtctcggcggtcgcggcccgctcggcggcggcgacct
cggcggccgcctggacctccgcgcggctggtcgacgcattcacctcggtacgggcggcttggacggctccagcggcctt
aaggcgggcgatctcggcggcggcggcccgctcctgcttgcgggcctcctcggcgagcctggcgtcctcctcggcctcc
cggagggcctgcgcgcgctcctcggcctgccgggggagcgccagggcctccgcgatggtcaggccgtaccgctccatgg
tgaggggcagccggacgtccgagggggccttgcggacgctgccgtattcgcgttccagcatgacgcggtagacgcgcag
ctccttgtccagctccacggcctccgcgtaggtggcgaccgcccacagcttcatgcggcgccacagggcgaaggtcggg
gcgggggcgaggaaccagcggtagagcgggacgcccgggccgttgtgggcgtcggggcggaccacgcggtcaacggcgg
tcttgccgacctccacgatggcgatgaacagcacgggcacgatcgcgtgggcgaacgtggccacgtagtccagggcggt
ccaggcgccggccgacggggcggcggaggccgcgttgaggacgatcgttgcgccggtcagcagccacacgggaaagcgg
acccagccgatggggcggcgcagccacgccatgagcaggtcgagggcgatcaggaccaggatgccgacgtcgacgccga
ccgtgaacagccgggccttgcccagcgtggcgaacccgagccggtcgtgcgcgaacgtggcgacgtgctcgaaggagag
gtagaggccgatcccggcgagcgccagggcggcgaggacgatcgctccggcggtggccgactgcgcccgggtgagcccg
ccggggagactccagcggcggcccgtgcccggagccgtcgcggtcgtctccggagccggggcggccgggctcgaagcgg
cggccgtcgtggccgaagccgtgggcggcgcgtccgaagcggggcgcggcgaggacggagtggggccggggcgctgctt
gctgaggggcagggtggtgagggtcatcaggtcctccgggtgatggtcgggcgaagtgtccgaagcggcggccgccgcc
cccgaagccggggtgtggcggccgccgtggcgcccgaaggtcggtcaggtccggccgtctcgcccggcggtgaggacca
gggcgagggtggcgagcgcggcgccgtacgccgacaagcaggtgagcaccgtgggcacggacggctggacgattgagac
ggccagccaggccgtggcgtgcacggtgaggaccaggacggcggcggcctcgcggacggtcagccgacgcggaggccgg
cggccggtcaccatcggcgctgccggatgacccggcggcgggcggcggggtcccggggcagcggcggcatcggggtcgg
cgtcggcacgacgacgatgcggcggcgctgcggggggacggtgcgctgcggctgctcggggcggtcgggcacggcggtc
accgcttgccctggcggacgcggcgcagggcctcgtccatgtccgggtcggagtcggtggggcaggtggccaggttgtt
ggtacggtccgtcatgcgtcgttcctcctggtgattcggttggtgcgcgcgggcacggcccccggttcaggcgctgcta
tcgcctcgggggccgtcgtcgtgtctggtcagccgcgggggccgggaaggccggcgccccggtggcgggggcactggca
gggcggccaggggccgggcccaaggcccagcggccccgcgttctcccatgccgtgtcgtggtcgacggcctcggtcacg
gcgctgccgtacgagcggccgccgtccgtgctcacccggaggtcatccgggagagtgtgccgccggggtccgggtgctc
gcggagctggtcccggatggcgtcggcggtgcggccgtagagcggctggtcgatcgggtgcgcgtgaacgtcatcggtc
atgcggtcggtccctctcgtctcgtgcgaaggcccccggcgggtgccgggggccggtggggggtgaggtctggtcaggc
ggcgagctgcgccgtggcggcctcgtcggcggccgggaagggcacgacgtcagcggcggcccgggtgacgcgcagcgag
ggcgcgcaggtccgcatggggacgtccccgagcccgagccgcttgtaggtggcgcggatggcctccaggtccggggtct
gccgcgaggacgggacgcgctccacggtccagacgccgtaacgcccggcgggaaggcggtccaggagcttgcgggcctt
gcgcttgccccggtctccggagcgggcccggtcggtggcggcggcgtagtcggctgccgcgtcctcgatctcctcggcg
cgggcctcgatctcctccggagccaggaggccggtcgggtcgggcgccgtctcgtcggcgcgggagcggaccagggagg
ccggggccacggtggagcggacggcgtcggcgacggtgcggaaggcgcgacgcgcgcgggtggtgacgctacggatcat
ggtggtgcctcctggtgactggtgctggtcgtgcgagggccaccggggcgctgctatcgcccctggcggccggggtgcc
gagtaggcgcggctccggcacgacgcgcgtgacgcgcgaggtgctggaccgcgacgactcggagccgtcggtgccggtc
ttcaccgccatgtttccgacctcccacacgctcgaaggctggcggtccacgggttaccggcgtggtccctgatctgcgg
ttatcaacgtgctgtcgcttccttcgagaccaccgtcagtgctgtccctccgggcgttgagtgatcacctcgcttcggt
gcgtggtggcccctctgggcttaagtagagcactacacttaaggaatcgcaagcactacactcaagagcgtcagcacga
tgagcaggagggaccgatggcgactaggcggaagggccgccctggcggctatgaggaaatcgccgcgcactttcggcgg
ctcatggactcgggcgagttgtcccctggcgacccgctgccctccatgcgcgacgtgtgcgaccagttcggttcggcga
tcacgacggtgaaccgggcgttccggctcctccaggaggagggccggacggtctccaagccgggcgtgggcacgatcgt
ccgggacatgtcccgggttcgggtgccgttcagtacgtacggcgacgtcctggcgccgggcggcgataagggcccgtgg
gagcgtgcgacggccgcgcagggccttgacggccggatgctcgtggaggcgcccgaggaggtcggggccccggcggacg
tcgccgcgcgcctcggcatcgagccgggcgccctggtcgtccaccggcggcgccgcgccacgatcggcgaggacgtcgt
ccagctccaagacgcctggtacccgctggagatcgcccgggccgccggcctggaccggccggggaaggtcgtgggtggt
gtcctcggtgccatgacgggcgccggcctttcgccgacgtccaccgaccacgacgtcgaggtgtgggtgccgtccgcgc
agcaagccgcggaactctccctcggctcccgcgtgtcggtcctggtcgtcgagcgcgtcacctacgacgcgacggtccg
tgtcctggaactgacccgtcacacgggcgcggctgacaggctgacgctgacctacaagggcctgccactccgggcgacc
ggagccgaggggagcacgtcatgacgaacgagctggacaccgccagcgcggccgcgcaggagacgctggagaggtcggc
ggcggaactggtccgcctccaggtggtgcgggagctacgcgccacgcacggcctggtctaccgggcgctggacggcatg
gagccgccctatctggtggcgtgggagggccggttcgttggcgaggtgcaccggcacggtccggcctatctgcactcct
ggtacgtgctgccgtacggcgacgacgaggagccgacggcggaggcccccgacttcaacggccgtccgttcggcacggc
ccgggccgccgccgcgtcgctcaagccaggccaggcgtaggccggccccgcccggacacgagaccgccccccttccgca
gccaccggaacgggggcggtcctatgccgcaccagagccaacacacccttggaggatcaccccgtgacccaatcccgga
agccctcggaagtcgtctatctcttaggagccgagggcctagacctggtgaagatcggcaccacgacagacgtggagag
gagagtccgcacgatgcagaccggtctgccgctgacgctgtcggtgctgtggacgtgcgagggcggccgcgcccttgag
ggggcgctccaccaggagttccgtgagcacaaccggcggggcgagtggttcgacctgaccagccttggtgacccggtgg
ccgtcgtgagtgagacggtgcggaggcttgcgccaggtcttgggctcccgatcccgccgccgcgcgtctcgccggacct
ggctctaacgcctgtggcccccacgattccggggtgcgtccggctggagcgcaccctgaccgctcggatgctcgcctgg
ttcgagtgcgaagacttcggccttccgccctccgcgagcaagcctgtgatgctgtaccggaccgtccagtacgacgcga
agggcgacgtcctggccgtgacgcagacgctcgttggcgatggacacgagcagcctgaaggcttcgagcttcctcccgt
acagggcggcaactgaccgtctggcacatagatcagcgcccccgctccgtgggagatcggagcgggggcgctgtgttgt
gcggtcaagagacggggcggatcgtccggcggcggcgagcgtcggtgttgctgaccttgcggggcgcgcgggcacggtc
gtccggggtgaccggggggtccgtgagggcggcggtgatcatctcgcgcagctcggggtgcagcgtgaagtagtaccgg
gcgttgatccggtagaggccggcggaggtccgcacgaggaggttcagggcctccaggcgggcgagagcctcgtgcaccg
tggagacggagttgccgcagtcctcggcgacggtttcgaccggtacggggatgacgtcaaacaggccgccgtggtcctc
cacccacgcgagcgtgcagtgctgagcggcggacaggttcggcgagtacagcgacggggcggtcaggtcgcgctccggg
ttgaagccttctacgggcatggtcgtcatcgcgtctctcctgcggcgcggcggcgggtagccgggatggtcttgggctt
gctggcggacctggtcatggccttgtcgtccggggccacacgcggcggcgccatgcggcggcaggcgttgacctgcgtg
ctcccggctccctcccagtatcggagggggttcagctggtaagagccggatcgcgggcccggcttgaccacaagcccga
gcttgaccagccggttaagggccttccggcaggtgtccttgctggcgccgacgtcctcgcccagctcctcgggcgtgac
cgggacgtatccggccgcgcctccgtgttcgcggagccattcgagcgtcgcccactccgctgcggccagcccgagggag
tacagggggacgtcgctgcctacggcctgcaaggtgtaccccttcccgtcgaactggtgcggagtccgcggcctggcgg
acaccgcctctccggtgtccatgttgaccagggccatgttcggcccgcgcttcctcgtcggcactcgcgcctctcccat
cctcgcgctaaggggcggtttttgaccccttagctccgatgaaccgccgcttacgagaggaaactaacaggtcaccact
ccgaaaaacagccgcttagtccgcgtgtcacacgcataggtgacgcgacttcctggcgaccgggcgcgacgagggacgc
acggtgggtagctaagcggcgatttttgacccagtaaccggcggtttttagccgcttagccgcgcgttttaccaggtca
agagcatgcgccccttcttcactagaaggcgcgcgccgtcactcacgcaccacggcgcacaccgtggtgaccgccctgg
actcctacccgcccggcataccgggccgctcttagcttctacggggaaggccggcggtcctgcgcacctggtcctcgtc
ggcgcctacggtcggcgagtcctcagccctggcgggcttgcgggtcccgactgatgccacctgatcacgcctggcgacc
gcacgctgtcctcggccggccacaagcaggaaggaagcgtcgtcctgccggtgggcggctgaggtacctccgtaccgcc
gccgttgtcgcccccgccgcaggggttgcctttctgtggctgaggtcgattcccccgccattgatcacccgcaccaggg
agggcccagccgacgaggtggcgcgcgtgcgcacgtcgctgggtgggggtgcggtaggtaccgggcagcagctcgggga
ccaggccccggcgggcgggggtccggaggagcccgccgggggtgtgcgcaagggcgtagcccgtagccccggagcgcag
cggaggggtcgctagtaggaggccgctatggcgcgtaccaccagcccacagggtccggcctccggaggcgacgcactgc
cggaccggaccgcaaggtccggtgagcccacaacggcggatcaaccgataagacggccaccgggcaggaagagggtgta
cgcgccccttggtggcccctctcgcgcgcgatcacctcacggcgcgtctcccaggtagtcagatccgtgcggggttctc
tacgggccgcacacggccttcgtcctaagaggctcttttccgccgcttagctccgaggaaccgccccttatctcaatga
aagcgcaggtcaccactccaggaaacggccgggcgagcgaccagccaggaggggagccgcggatgcgccggtcctcgac
cgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgac
tgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctgg
agcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccg
ccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgtt
cgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggcc
atgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcga
tcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgc
cgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggc
ggcacctcgctaacggattcaccactccaagaattggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaa
cccttggcagaacatatccatcgcgtccgccatctccagcagccgcacgcggcgcatctcgggccgcgttgctggcgtt
tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggacta
taaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgt
ccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc
aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtg
ctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagcc
agttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc
aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtgga
acgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatg
aagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatc
tcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttac
catctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagc
cggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga
gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcagcaggcatcgtggtgtcacgctcgtcgtttg
gtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttag
ctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataat
tctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgta
tgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcat
cattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgt
gcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaa
aaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcaggg
ttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccga
aaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttc
gtcttcaatgcatctatgaattcgcggccgcttctagag