BBa_M11421 1 BBa_M11421 Streptomyces Shuttle Vetor Thiostrepton Resistance 2010-04-25T11:00:00Z 2015-05-08T01:13:54Z This originated from the plasmid pRM5. Site-directed mutagenesis was used to remove specific restriction sites to make it biobrick compatible. This is a shuttle vector to be used with E. coli and Streptomyces. It has thiostrepton resistance. false false _572_ 0 4981 9 Not in stock false This is a shuttle vector for Streptomyces. false Amanda Romero annotation2068723 1 Start Codon range2068723 1 382 384 annotation2068720 1 Suffix range2068720 1 1 21 annotation2068721 1 Prefix range2068721 1 11867 11889 annotation2068724 1 Stop Codon range2068724 1 1189 1191 annotation2068722 1 Thiostrepton Resistance range2068722 1 382 1188 annotation2068725 1 oriSCP2 range2068725 1 1554 8594 BBa_M11421_sequence 1 tactagtagcggccgctgcagaagcttcggccacgacacccccatcggcatcgcgtggcgggcccgattcgcgtccgccccggggaggagacgggcgtccgcgtcgagggcgaggcgggcttccacccgtaggcgatccggcgagcacgacacccgcagattggctcgacgcagcccagaaatgtatgatcaaggcgaatacttcatatgcggggatcgaccgcgcgggtcccggacggggaagagcggggagctttgccagagagcgacgacttccccttgcgttggtgattgccggtcagggcagccatccgccatcgtcgcgtagggtgtcacaccccaggaatcgcgtcactgaacacagcagccggtaggacgaccatgactgagttggacaccatcgcaaatccgtccgatcccgcggtgcagcggatcatcgatgtcaccaagccgtcgcgatccaacataaagacaacgttgatcgaggacgtcgagcccctcatgcacagcatcgcggccggggtggagttcatcgaggtctacggcagcgacagcagtccttttccatctgagttgctggatctgtgcgggcggcagaacataccggtccgcctcatcgactcctcgatcgtcaaccagttgttcaagggggagcggaaggccaagacattcggcatcgcccgcgtccctcgcccggccaggttcggcgatatcgcgagccggcgtggggacgtcgtcgttctcgacggggtgaagatcgtcgggaacatcggcgcgatagtacgcacgtcgctcgcgctcggagcgtcggggatcatcctggtcgacagtgacatcaccagcatcgcggaccggcgtctccaaagggccagccgaggttacgtcttctcccttcccgtcgttctctccggtcgcgaggaggccatcgccttcattcgggacagcggtatgcagctgatgacgctcaaggcggatggcgacatttccgtgaaggaactcggggacaatccggatcggctggccttgctgttcggcagcgaaaagggtgggccttccgacctgttcgaggaggcgtcttccgcctcggtttccatccccatgatgagccagaccgagtctctcaacgtttccgtttccctcggaatcgcgctgcacgagaggatcgacaggaatctcgcggccaaccgataagcgcctctgttcctcggacgctcggttcctcgacctcgattcgtcagtgatgatcacctcacacggcagcgatcaccactgacatatcgaggtcaacggtcgtggtccgggcgggcactcctcgaaggcgcggccgacgcccttgaacgactcgatggcgctgacgccttccgcgatcgcctcgactgtgaaaacgctcgtggacgtcggcgggtagccgatgagttccatcgccttgcgcatccccttgagcatggagaacgcgtccaccgggtagtcacgaaggtacttgcgaaggcttcgcgccgtccgagaccgtgagccggcgcaagccggtgtggcgccggtcccggatttgcaggcatgcctcgagtccgcagtcctggcaggcagagcaggtccggcagtggccacagccgcggttgtcgggcggccagcagcggcagccggtgggctgccaggggtcggcggagccgaccagcccgagctggccgggcacgttctcctcgggcgctcggcgcggcgcgcgtggcttgacgcggctgcgggccatcagcgggcctccggggtgtagccctcggcgaggagctgcgcggcggccgaccggtactccgaggccgtcgtctgcgagacggagcaggcggtcatgaccgactccagggggaggttctccgggtgtccggcgccctcggcgaggatcatccgggccaccttgcggaccttgcggtcacgcggggagagggtcagttcgtcctcggcttcgaccgcccggcgctccgcttcggccgcgcggtggcgggcttcggcggcggcctgctcggcttcggctgcgtcccgctgtgcctgggaggcgcgggcccgggcctcctcggtgctggcctcggcggccgccgtgcgggcctcgtcttcggccgcagtgagggccgcttcggcttcgcgggccctggcttcggctgcggcctcgtcggcttcggccgcgcggcggcgggcttcggcggcgcggtgctcggcggccgcgcggtcggcttcggcccgggcggcggcctccgactggacggcctccgtctcggcggtcgcggcccgctcggcggcggcgacctcggcggccgcctggacctccgcgcggctggtcgacgcattcacctcggtacgggcggcttggacggctccagcggccttaaggcgggcgatctcggcggcggcggcccgctcctgcttgcgggcctcctcggcgagcctggcgtcctcctcggcctcccggagggcctgcgcgcgctcctcggcctgccgggggagcgccagggcctccgcgatggtcaggccgtaccgctccatggtgaggggcagccggacgtccgagggggccttgcggacgctgccgtattcgcgttccagcatgacgcggtagacgcgcagctccttgtccagctccacggcctccgcgtaggtggcgaccgcccacagcttcatgcggcgccacagggcgaaggtcggggcgggggcgaggaaccagcggtagagcgggacgcccgggccgttgtgggcgtcggggcggaccacgcggtcaacggcggtcttgccgacctccacgatggcgatgaacagcacgggcacgatcgcgtgggcgaacgtggccacgtagtccagggcggtccaggcgccggccgacggggcggcggaggccgcgttgaggacgatcgttgcgccggtcagcagccacacgggaaagcggacccagccgatggggcggcgcagccacgccatgagcaggtcgagggcgatcaggaccaggatgccgacgtcgacgccgaccgtgaacagccgggccttgcccagcgtggcgaacccgagccggtcgtgcgcgaacgtggcgacgtgctcgaaggagaggtagaggccgatcccggcgagcgccagggcggcgaggacgatcgctccggcggtggccgactgcgcccgggtgagcccgccggggagactccagcggcggcccgtgcccggagccgtcgcggtcgtctccggagccggggcggccgggctcgaagcggcggccgtcgtggccgaagccgtgggcggcgcgtccgaagcggggcgcggcgaggacggagtggggccggggcgctgcttgctgaggggcagggtggtgagggtcatcaggtcctccgggtgatggtcgggcgaagtgtccgaagcggcggccgccgcccccgaagccggggtgtggcggccgccgtggcgcccgaaggtcggtcaggtccggccgtctcgcccggcggtgaggaccagggcgagggtggcgagcgcggcgccgtacgccgacaagcaggtgagcaccgtgggcacggacggctggacgattgagacggccagccaggccgtggcgtgcacggtgaggaccaggacggcggcggcctcgcggacggtcagccgacgcggaggccggcggccggtcaccatcggcgctgccggatgacccggcggcgggcggcggggtcccggggcagcggcggcatcggggtcggcgtcggcacgacgacgatgcggcggcgctgcggggggacggtgcgctgcggctgctcggggcggtcgggcacggcggtcaccgcttgccctggcggacgcggcgcagggcctcgtccatgtccgggtcggagtcggtggggcaggtggccaggttgttggtacggtccgtcatgcgtcgttcctcctggtgattcggttggtgcgcgcgggcacggcccccggttcaggcgctgctatcgcctcgggggccgtcgtcgtgtctggtcagccgcgggggccgggaaggccggcgccccggtggcgggggcactggcagggcggccaggggccgggcccaaggcccagcggccccgcgttctcccatgccgtgtcgtggtcgacggcctcggtcacggcgctgccgtacgagcggccgccgtccgtgctcacccggaggtcatccgggagagtgtgccgccggggtccgggtgctcgcggagctggtcccggatggcgtcggcggtgcggccgtagagcggctggtcgatcgggtgcgcgtgaacgtcatcggtcatgcggtcggtccctctcgtctcgtgcgaaggcccccggcgggtgccgggggccggtggggggtgaggtctggtcaggcggcgagctgcgccgtggcggcctcgtcggcggccgggaagggcacgacgtcagcggcggcccgggtgacgcgcagcgagggcgcgcaggtccgcatggggacgtccccgagcccgagccgcttgtaggtggcgcggatggcctccaggtccggggtctgccgcgaggacgggacgcgctccacggtccagacgccgtaacgcccggcgggaaggcggtccaggagcttgcgggccttgcgcttgccccggtctccggagcgggcccggtcggtggcggcggcgtagtcggctgccgcgtcctcgatctcctcggcgcgggcctcgatctcctccggagccaggaggccggtcgggtcgggcgccgtctcgtcggcgcgggagcggaccagggaggccggggccacggtggagcggacggcgtcggcgacggtgcggaaggcgcgacgcgcgcgggtggtgacgctacggatcatggtggtgcctcctggtgactggtgctggtcgtgcgagggccaccggggcgctgctatcgcccctggcggccggggtgccgagtaggcgcggctccggcacgacgcgcgtgacgcgcgaggtgctggaccgcgacgactcggagccgtcggtgccggtcttcaccgccatgtttccgacctcccacacgctcgaaggctggcggtccacgggttaccggcgtggtccctgatctgcggttatcaacgtgctgtcgcttccttcgagaccaccgtcagtgctgtccctccgggcgttgagtgatcacctcgcttcggtgcgtggtggcccctctgggcttaagtagagcactacacttaaggaatcgcaagcactacactcaagagcgtcagcacgatgagcaggagggaccgatggcgactaggcggaagggccgccctggcggctatgaggaaatcgccgcgcactttcggcggctcatggactcgggcgagttgtcccctggcgacccgctgccctccatgcgcgacgtgtgcgaccagttcggttcggcgatcacgacggtgaaccgggcgttccggctcctccaggaggagggccggacggtctccaagccgggcgtgggcacgatcgtccgggacatgtcccgggttcgggtgccgttcagtacgtacggcgacgtcctggcgccgggcggcgataagggcccgtgggagcgtgcgacggccgcgcagggccttgacggccggatgctcgtggaggcgcccgaggaggtcggggccccggcggacgtcgccgcgcgcctcggcatcgagccgggcgccctggtcgtccaccggcggcgccgcgccacgatcggcgaggacgtcgtccagctccaagacgcctggtacccgctggagatcgcccgggccgccggcctggaccggccggggaaggtcgtgggtggtgtcctcggtgccatgacgggcgccggcctttcgccgacgtccaccgaccacgacgtcgaggtgtgggtgccgtccgcgcagcaagccgcggaactctccctcggctcccgcgtgtcggtcctggtcgtcgagcgcgtcacctacgacgcgacggtccgtgtcctggaactgacccgtcacacgggcgcggctgacaggctgacgctgacctacaagggcctgccactccgggcgaccggagccgaggggagcacgtcatgacgaacgagctggacaccgccagcgcggccgcgcaggagacgctggagaggtcggcggcggaactggtccgcctccaggtggtgcgggagctacgcgccacgcacggcctggtctaccgggcgctggacggcatggagccgccctatctggtggcgtgggagggccggttcgttggcgaggtgcaccggcacggtccggcctatctgcactcctggtacgtgctgccgtacggcgacgacgaggagccgacggcggaggcccccgacttcaacggccgtccgttcggcacggcccgggccgccgccgcgtcgctcaagccaggccaggcgtaggccggccccgcccggacacgagaccgccccccttccgcagccaccggaacgggggcggtcctatgccgcaccagagccaacacacccttggaggatcaccccgtgacccaatcccggaagccctcggaagtcgtctatctcttaggagccgagggcctagacctggtgaagatcggcaccacgacagacgtggagaggagagtccgcacgatgcagaccggtctgccgctgacgctgtcggtgctgtggacgtgcgagggcggccgcgcccttgagggggcgctccaccaggagttccgtgagcacaaccggcggggcgagtggttcgacctgaccagccttggtgacccggtggccgtcgtgagtgagacggtgcggaggcttgcgccaggtcttgggctcccgatcccgccgccgcgcgtctcgccggacctggctctaacgcctgtggcccccacgattccggggtgcgtccggctggagcgcaccctgaccgctcggatgctcgcctggttcgagtgcgaagacttcggccttccgccctccgcgagcaagcctgtgatgctgtaccggaccgtccagtacgacgcgaagggcgacgtcctggccgtgacgcagacgctcgttggcgatggacacgagcagcctgaaggcttcgagcttcctcccgtacagggcggcaactgaccgtctggcacatagatcagcgcccccgctccgtgggagatcggagcgggggcgctgtgttgtgcggtcaagagacggggcggatcgtccggcggcggcgagcgtcggtgttgctgaccttgcggggcgcgcgggcacggtcgtccggggtgaccggggggtccgtgagggcggcggtgatcatctcgcgcagctcggggtgcagcgtgaagtagtaccgggcgttgatccggtagaggccggcggaggtccgcacgaggaggttcagggcctccaggcgggcgagagcctcgtgcaccgtggagacggagttgccgcagtcctcggcgacggtttcgaccggtacggggatgacgtcaaacaggccgccgtggtcctccacccacgcgagcgtgcagtgctgagcggcggacaggttcggcgagtacagcgacggggcggtcaggtcgcgctccgggttgaagccttctacgggcatggtcgtcatcgcgtctctcctgcggcgcggcggcgggtagccgggatggtcttgggcttgctggcggacctggtcatggccttgtcgtccggggccacacgcggcggcgccatgcggcggcaggcgttgacctgcgtgctcccggctccctcccagtatcggagggggttcagctggtaagagccggatcgcgggcccggcttgaccacaagcccgagcttgaccagccggttaagggccttccggcaggtgtccttgctggcgccgacgtcctcgcccagctcctcgggcgtgaccgggacgtatccggccgcgcctccgtgttcgcggagccattcgagcgtcgcccactccgctgcggccagcccgagggagtacagggggacgtcgctgcctacggcctgcaaggtgtaccccttcccgtcgaactggtgcggagtccgcggcctggcggacaccgcctctccggtgtccatgttgaccagggccatgttcggcccgcgcttcctcgtcggcactcgcgcctctcccatcctcgcgctaaggggcggtttttgaccccttagctccgatgaaccgccgcttacgagaggaaactaacaggtcaccactccgaaaaacagccgcttagtccgcgtgtcacacgcataggtgacgcgacttcctggcgaccgggcgcgacgagggacgcacggtgggtagctaagcggcgatttttgacccagtaaccggcggtttttagccgcttagccgcgcgttttaccaggtcaagagcatgcgccccttcttcactagaaggcgcgcgccgtcactcacgcaccacggcgcacaccgtggtgaccgccctggactcctacccgcccggcataccgggccgctcttagcttctacggggaaggccggcggtcctgcgcacctggtcctcgtcggcgcctacggtcggcgagtcctcagccctggcgggcttgcgggtcccgactgatgccacctgatcacgcctggcgaccgcacgctgtcctcggccggccacaagcaggaaggaagcgtcgtcctgccggtgggcggctgaggtacctccgtaccgccgccgttgtcgcccccgccgcaggggttgcctttctgtggctgaggtcgattcccccgccattgatcacccgcaccagggagggcccagccgacgaggtggcgcgcgtgcgcacgtcgctgggtgggggtgcggtaggtaccgggcagcagctcggggaccaggccccggcgggcgggggtccggaggagcccgccgggggtgtgcgcaagggcgtagcccgtagccccggagcgcagcggaggggtcgctagtaggaggccgctatggcgcgtaccaccagcccacagggtccggcctccggaggcgacgcactgccggaccggaccgcaaggtccggtgagcccacaacggcggatcaaccgataagacggccaccgggcaggaagagggtgtacgcgccccttggtggcccctctcgcgcgcgatcacctcacggcgcgtctcccaggtagtcagatccgtgcggggttctctacgggccgcacacggccttcgtcctaagaggctcttttccgccgcttagctccgaggaaccgccccttatctcaatgaaagcgcaggtcaccactccaggaaacggccgggcgagcgaccagccaggaggggagccgcggatgcgccggtcctcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccactccaagaattggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaacccttggcagaacatatccatcgcgtccgccatctccagcagccgcacgcggcgcatctcgggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcagcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaatgcatctatgaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z