LOCUS       BBa_M11501              1335 bp    DNA     linear       15-Jun-2024
DEFINITION  As induced GFP reporter
ACCESSION   BBa_M11501
VERSION     BBa_M11501.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=ArsR Binding Promoter
     RBS             108..112
                     /label=Ribosome Binding Site
     CDS             119..472
                     /label=ArsR
     misc_feature    1..472
                     /label=BBa_J33100
     promoter        1..472
                     /label=BBa_J33100
     misc_feature    479..481
                     /label=Start
     CDS             479..1198
                     /label=GFP Coding Region
     CDS             479..1198
                     /label=BBa_K208000
     stem_loop       1218..1261
                     /label=stem_loop
     misc_feature    1207..1286
                     /label=BBa_B0010
     terminator      1207..1286
                     /label=BBa_B0010
     stem_loop       1302..1321
                     /label=T7 TE
     misc_feature    1328..1328
                     /label=stop
     polyA_site      1322..1335
                     /label=polya
     misc_feature    1295..1335
                     /label=BBa_B0012
     terminator      1295..1335
                     /label=BBa_B0012
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aatactagat
      481 ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      541 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg ctacatacgg
      601 aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      661 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
      721 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
      781 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
      841 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
      901 actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      961 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc aactagcaga
     1021 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1081 cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct
     1141 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaataata
     1201 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1261 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1321 ctttctgcgt ttata
//