LOCUS       BBa_M11512               968 bp    DNA     linear       26-Dec-2024
DEFINITION  Copper Promoter (CueR regulated) with YFP Reporter
ACCESSION   BBa_M11512
VERSION     BBa_M11512.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=-35 and -10 region
     misc_feature    3..28
                     /label=CueR binding site
     promoter        1..43
                     /label=BBa_K190017
     misc_feature    56..59
                     /label=conserved
     RBS             52..63
                     /label=BBa_B0034
     CDS             70..831
                     /label=YFP (LVA)
     misc_feature    826..831
                     /label=2
     misc_feature    787..825
                     /label=SsrA
     misc_feature    311..311
                     /label=A (Q->L)
     misc_feature    70..831
                     /label=BBa_E0032
     misc_feature    70..831
                     /label=BBa_E0032
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
ORIGIN
        1 ttgaccttcc cgtaagggga aggactatgc tcaacgtttg atttactaga gaaagaggag
       61 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      241 tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac
      301 cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      661 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      781 tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttata
//