LOCUS       BBa_M11514               912 bp    DNA     linear       20-Dec-2024
DEFINITION  Copper Promoter (CueR regulated) with RFP Reporter
ACCESSION   BBa_M11514
VERSION     BBa_M11514.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=-35 and -10 region
     misc_feature    3..28
                     /label=CueR binding site
     promoter        1..43
                     /label=BBa_K190017
     misc_feature    56..59
                     /label=conserved
     RBS             52..63
                     /label=BBa_B0034
     CDS             70..744
                     /label=mrfp1
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             70..775
                     /label=BBa_E1010
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     stem_loop       879..898
                     /label=T7 TE
     misc_feature    905..905
                     /label=stop
     polyA_site      899..912
                     /label=polya
     misc_feature    872..912
                     /label=BBa_B0012
     terminator      872..912
                     /label=BBa_B0012
ORIGIN
        1 ttgaccttcc cgtaagggga aggactatgc tcaacgtttg atttactaga gaaagaggag
       61 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      121 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
      181 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
      241 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
      301 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
      361 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
      421 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
      481 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
      541 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
      601 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
      661 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
      721 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta ta
//