LOCUS       BBa_M11524               994 bp    DNA     linear       22-Nov-2024
DEFINITION  Zinc Promoter (ZntR regulated) with YFP
ACCESSION   BBa_M11524
VERSION     BBa_M11524.1  
FEATURES             Location/Qualifiers
     promoter        35..69
                     /label=-35 and -10 region
     misc_feature    32..53
                     /label=Palindromic binding site for ZntR
     promoter        1..69
                     /label=BBa_K190016
     misc_feature    82..85
                     /label=conserved
     RBS             78..89
                     /label=BBa_B0034
     CDS             96..857
                     /label=YFP (LVA)
     misc_feature    852..857
                     /label=2
     misc_feature    813..851
                     /label=SsrA
     misc_feature    337..337
                     /label=A (Q->L)
     misc_feature    96..857
                     /label=BBa_E0032
     misc_feature    96..857
                     /label=BBa_E0032
     stem_loop       877..920
                     /label=stem_loop
     misc_feature    866..945
                     /label=BBa_B0010
     terminator      866..945
                     /label=BBa_B0010
     stem_loop       961..980
                     /label=T7 TE
     misc_feature    987..987
                     /label=stop
     polyA_site      981..994
                     /label=polya
     misc_feature    954..994
                     /label=BBa_B0012
     terminator      954..994
                     /label=BBa_B0012
ORIGIN
        1 cgtccgctcg ctgtatctct gataaaactt gactctggag tcgactccag agtgtatcct
       61 tcggttaatt actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt
      121 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      181 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      241 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
      301 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca
      361 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      421 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      481 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      541 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
      601 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      661 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga
      721 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      781 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg
      841 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      901 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      961 ggctcacctt cgggtgggcc tttctgcgtt tata
//