LOCUS       BBa_M11531              1382 bp    DNA     linear       30-Jun-2024
DEFINITION  Lead promoter with GFP
ACCESSION   BBa_M11531
VERSION     BBa_M11531.1  
FEATURES             Location/Qualifiers
     promoter        1..94
                     /label=BBa_I721001
     misc_feature    107..110
                     /label=conserved
     RBS             103..114
                     /label=BBa_B0034
     CDS             121..519
                     /label=BBa_I721002
     misc_feature    526..528
                     /label=Start
     CDS             526..1245
                     /label=GFP Coding Region
     CDS             526..1245
                     /label=BBa_K208000
     stem_loop       1265..1308
                     /label=stem_loop
     misc_feature    1254..1333
                     /label=BBa_B0010
     terminator      1254..1333
                     /label=BBa_B0010
     stem_loop       1349..1368
                     /label=T7 TE
     misc_feature    1375..1375
                     /label=stop
     polyA_site      1369..1382
                     /label=polya
     misc_feature    1342..1382
                     /label=BBa_B0012
     terminator      1342..1382
                     /label=BBa_B0012
ORIGIN
        1 ggttgcttcc tataaaaaac ttgactctat atctactaga ggttttctaa tgatggcatc
       61 cggggaaaac cttgtcaatg aagagcgatc tatgtactag agaaagagga gaaatactag
      121 atgatgcgga tcggtgaact gggcaagaag gcagattgct tggtgcagac cgtgcgcttt
      181 tacgagtcag aaggcttgct gcccgagcct gcacgtagcg agggcaactt caggctctat
      241 gacgaagtcc atttgcagcg cttgctgttc atccgccgct gccgggcgaa ggacatgacg
      301 ctggatgaga tccgtcaact gctgaactta cgggatcggc cagagttggg ctgcggcgag
      361 gtgaacgcgc tggtcgacgc tcatatcgcg caagtgcgga ccaagatgaa ggaattgcgc
      421 gccttggagc gcgagttaat ggatctgcga cgctcctgcg atagcgcccg aacctcgcgc
      481 gagtgcggca ttctcaacag cttggccgag cccgcctgat actagatggc tagcaaagga
      541 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      601 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt
      661 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      721 tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc
      781 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg
      841 aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag
      901 ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac
      961 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac
     1021 ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa
     1081 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa
     1141 tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta
     1201 actgctgctg ggattacaca tggcatggat gagctctaca aataatacta gagccaggca
     1261 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1321 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1381 ta
//