BBa_M11544 1 BBa_M11544 Fe (iron) Promoter (point mutations) 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z Part BBa_I765000 This promoter is a mutated version of part BBa_I765000 at all of its PstI restriction sites. false false _572_ 0 6772 9 Not in stock false There were several PstI restriction sites that had to be mutated. The six nucleotide sequence for PstI is ctgcag. We mutated it so that the sequence became ctacag, keeping the appropriate amino acid still in place, but getting rid of the potential for PstI to cut it. false Alyssa Calder BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M11542 1 BBa_M11542 Fe Promoter with RFP 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z BBa_M11544 and BBa_B0034 An iron promoter region, regulated by the presence of iron, with an RFP coding region. This allows detection of iron in a substance. false false _572_ 0 6772 9 Not in stock false none false Alyssa Calder component2256891 1 BBa_B0034 component2256894 1 BBa_E1010 component2256895 1 BBa_B0010 component2256897 1 BBa_B0012 component2256889 1 BBa_M11544 annotation2256891 1 BBa_B0034 range2256891 1 1053 1064 annotation2256894 1 BBa_E1010 range2256894 1 1071 1776 annotation2256895 1 BBa_B0010 range2256895 1 1785 1864 annotation2256889 1 BBa_M11544 range2256889 1 1 1044 annotation2256897 1 BBa_B0012 range2256897 1 1873 1913 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M11544_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctacaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctacaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctacaggcgatgaagcctgacctacagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaa BBa_M11542_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctacaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctacaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctacaggcgatgaagcctgacctacagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z